Preclinical evaluation of efficacy and safety of a new iron chelator therapy in chronic spinal cord injury

新型铁螯合剂疗法治疗慢性脊髓损伤的临床前疗效和安全性评价

• 批准号: 10701817
• 负责人: PRODIP K. BOSE
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2026-09-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10701817
• 关键词: Acceleration; Accidents; Achievement; Address; Animal Model; Animals; Attenuated; Axon; Behavioral; Bicycling; Biological Assay; Blood; Blood Vessels; Cells; Cervical spinal cord injury; Chronic; Clinical Trials; Complementary therapies; Contusions; Corticospinal Tracts; Data; Deceleration; Deposition; Deterioration; Diffuse; Diffuse Axonal Injury; Diffusion Magnetic Resonance Imaging; Disease; Dorsal; Dose; Endothelium; Evaluation; Excision; Exercise; Exercise Therapy; Florida; Functional disorder; Gait; Gliosis; Goals; Healthcare; Hemorrhage; Histologic; Human; Image; Individual; Inflammation; Inflammatory; Injury; Investigational Drugs; Investigational New Drug Application; Iron; Iron Chelation; Legal patent; Life; Magnetic Resonance Imaging; Measures; Mediating; Medical; Metabolic; Modeling; Motor; Motor Evoked Potentials; Nervous System Trauma; Neurologic; Neurology; Neuronal Plasticity; Neurons; Oral; Outcome Measure; Oxidative Stress; Pathway interactions; Pharmaceutical Preparations; Phase; Physiological; Placebo Control; Preclinical Testing; Publishing; Quality of life; Quantitative Evaluations; Reactive Oxygen Species; Regenerative capacity; Reporting; Risk Factors; Rodent; Rodent Model; Safety; Saline; Site; Source; Spinal Cord; Spinal Cord Contusions; Spinal cord injury; Sports; Symptoms; Technology; Testing; Thalassemia; Therapeutic; Time; Tissues; Toxic effect; Translations; Treatment Efficacy; United States Food and Drug Administration; Universities; Veterans; battlefield injury; behavior measurement; catalyst; cell injury; clinically relevant; cohort; design; disability; effective therapy; efficacy evaluation; functional improvement; healing; injured; innovation; nerve injury; neural; neuroinflammation; neuron loss; novel; patient oriented; phase I trial; pre-Investigational New Drug meeting; pre-clinical; preclinical evaluation; primary outcome; protective factors; rehabilitation strategy; reticulospinal tract; safety outcomes; safety testing; spasticity; therapeutically effective; therapy duration; translational potential; treatment duration; treatment effect; treatment optimization; treatment strategy; user-friendly

Cervical spinal cord injury (C-SCI) is a common and frequently devastating battlefield injury that can result in a broad range of life-long locomotor and spasticity disabilities. With advances in early evacuation and aggressive medical therapy, there are still no effective therapeutics that salvage spinal cord (SC) neurons /reduce progressive secondary damage. Acceleration/deceleration and contusion SCI cause micro-vessel shear injury, blood spinal cord barrier (BSCB) dysfunction, and hemorrhage. Iron deposited by diffuse micro-hemorrhage fuels oxidative stress and inflammation through reactive oxygen species (ROS), which further induce progressive disabilities. There is an urgent need to address both specific disabilities and risk factors for long-term progressive disabilities, and to develop effective therapies that have excellent potential for translation. The proposal will test the preclinical evaluation of the safety and efficacy of a new iron chelator, SP420, with or without a programmed locomotor therapy in a rodent model of contusion CSCI. The combination of two complementary therapies is aimed to amplify robustness necessary to significantly improve function in a chronic setting of SCI. This novel patented iron chelator will remove bleed-induced free toxic iron, a powerful catalyst of oxidative stress/inflammation, and with locomotor therapy it will upregulate neural and vascular trophic agents to protect and heal injured neural and vascular tissues. The long-term goal of these studies is to develop an effective SCI therapeutic, and to obtain sufficient preclinical evidence to support a Food and Drug Administration (FDA) Investigational New Drug (IND) application for human SCI clinical trials. Accordingly, Three Specific Aims will be tested in a clinically relevant rodent model of C-SCI. Currently, the drug has an IND for iron storage disease (e.g. Thalassemia). We have reported enduring motor (spasticity and gait) disabilities in this model. Specific Aim 1 (SA-1): Safety, efficacy and optimization of treatment duration. SP420 will be administered SQ at one fixed dose (80 mg/kg; represents the human phase II dose) in three different durations and tested against saline placebo controls. Treatment will be initiated at two post-injury chronic time points (post-injury week-4 and week-12), each using a separate cohort of animals. Quantitative physiological measures of spasticity, gait, and the integrity of axonal conduction of descending locomotor pathways functions are the primary outcomes along with clinically relevant T1/T2W, SWI/QSM, and DTI MRIs. A comprehensive list of safety outcomes will be assessed as well during the treatment. Specific Aim 2: To determine the efficacy of combined SP420 and locomotor exercise therapy in mitigating spasticity and gait disabilities. All outcome measures as stated in SA-1 will be applied. The functional/imaging/safety outcomes will be compared among the three treatment durations and two post-injury periods. Specific Aim 3 (SA-3): To determine SCI and treatment impacts on the temporal profile of iron toxicity/inflammation, cellular damage, BSCB integrity, and neuroplasticity (trophic factors). A cause-effect relationship between iron deposition, tissue damage and treatment effects of iron chelator will be studied using a combination of histological, track tracing, and immunohistochemical assays to evaluate bleed iron, oxidative stress, inflammation, markers for BSCB integrity, and neural, and vascular protective factors. We hypothesize that free bleed iron fuels oxidative stress and neuroinflammation through ROS which drives the progression of neurological damage and motor disabilities, in part. We predict that the proposed SP-420 therapy will reverse the iron-mediated neurological damage and delayed neurological sequelae. The combination of two complementary therapies will amplify robustness necessary to significantly improve function in a chronic setting of SCI. Achievement of these goals will provide innovative, non-invasive, and patient-centered technologies and treatments that will greatly facilitate treatment of veterans and civilian SCI.

颈脊髓损伤（C-SCI）是一种常见且经常造成毁灭性的战场损伤