Molecular & Cellular Analysis Core

UAB视觉科学中心核心-分子和细胞分析核心 项目/摘要 分子和细胞分析核心被批准为分子生物学/组织学核心，并已成为 积极和关键的核心为许多核心参与者超过15年。本周期的核心参与者 发表了178篇论文（比上一个周期增加11%），表现出出色的生产力。 在建议的供资周期内，在14个中度或广泛使用的核心参与者中，12个是NEI R 01资助的 (86另外两间正积极寻求新的NEI R 01-资助或有其他NEI支持。三 四个有限使用的核心参与者建议有NEI R 01资金，另一个有其他资金 视觉支持来源核心不断发展，以满足用户的需求， 增强.主要的机构支助使得能够购买新设备或升级某些设备， 核心设备，以帮助满足用户的需求，提供最新的设备。的重点 这一周期的目的是通过大规模更新一个宽领域的 显微镜和添加一个先进的分析软件套件。核心包括蛋白质和核酸 酸分析，组织学，现在先进的显微成像和分析。皮特勒博士，核心主任是 他是细胞和分子生物学以及生物化学方面的专家，专注于视网膜变性的动物模型。 并将继续担任核心主管Marina Gorbatyuk博士是AAV技术专家， 动物模型中的未折叠蛋白反应将继续担任副主任，协助核心 治理和指标分析。除了拥有先进的分析设备外，核心还具有高度的 受过良好教育，训练有素的博士科学家，博士何塞路易斯罗伊格作为实验室主任。他广博的知识和 经验基础和全面的科学技能是一个重要的资产。核心设备主要安装在 靠近大多数用户的专用空间，可全天候访问。使用调度中的冲突是 通过所有主要设备的综合在线调度程序将最小化。出色的机构支持 在许多方面都很关键：（1）提供容纳核心设备的设施和办公室， 实验室主任，（2）维护设备，（3）获得新设备以提高核心能力。 核心的使用被仔细监控，以确保核心参与者之间的使用优先级如下：NEI R 01- 受资助的参与者有第一优先使用权（1），其次是具有NIH新研究者身份的参与者， 正在积极寻求NEI R 01（2），然后由作为NEI关键人员合作的参与者 R 01并积极寻求PI NEI R 01的支持（3），最后是最近在最后一次 已获得NEI R 01资助三年，并正在积极寻求新的NEI R 01支持（4）。

UAB Center Core for Vision Science - Molecular & Cellular Analysis Core Project/Summary Abstract The Molecular & Cellular Analysis Core was approved as a molecular biology/histology core and has been an active and critical core for many Core participants for more than 15 years. Core participants in the current cycle have demonstrated outstanding productivity publishing 178 papers (an 11% increase over the previous cycle). For the proposed funding cycle, of the 14 moderate or extensive use Core participants, 12 are NEI R01-funded (86%) and the other two are actively seeking new NEI R01-funding or have other NEI support. Three of the four limited use Core participants proposed have NEI R01-funding and the other one has funding from other vision support sources. The core has continued to evolve to meet user needs warranting several core enhancements. Major institutional support has allowed the purchase of new equipment or the upgrade of some of the key Core equipment to help meet user needs by providing the most up to date equipment. The focus for this cycle was to enhance the capabilities of microscopic analysis through major update of a wide-field microscope and addition of an advanced analysis software suite. The Core encompasses protein and nucleic acid analysis, histology, and now advanced microscopy imaging and analysis. Dr. Pittler, the Core Director is an expert in cell and molecular biology, and biochemistry focusing on animal models of retinal degeneration and will continue as the Core Director. Dr. Marina Gorbatyuk, an expert in AAV technology and the study of the unfolded protein response in animal models will continue as the Associate Director to assist with Core governance and metrics analysis. In addition to having advanced equipment for analysis, the Core has a highly educated, well-trained PhD scientist, Dr. Jose Luis Roig as the Laboratory Director. His broad knowledge and experience base and overall scientific skill is a major asset. The Core equipment is primarily housed in dedicated space in close proximity to most users and is accessible 24/7. Conflicts in usage scheduling is minimized through a comprehensive online scheduler for all major equipment. Outstanding institutional support has been critical in many ways: (1) to provide the facilities that house Core equipment and an office for the laboratory director, (2) to maintain the equipment, (3) and to obtain new equipment to increase core capability. Core usage is carefully monitored to ensure priority of use among Core participants as follows: NEI R01- funded participants have first priority for use (1), followed by participants with NIH new investigator status that are actively seeking an NEI R01 (2), then by participants that are collaborating as key personnel on an NEI R01 and actively seeking lead PI NEI R01 support (3), and lastly by participants that have recently in the last three years been NEI R01-funded and are actively seeking new NEI R01 support (4).