Investigating the molecules and mechanisms of bacterial cell-cell interactions
研究细菌细胞间相互作用的分子和机制
基本信息
- 批准号:10686142
- 负责人:
- 金额:$ 41.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2027-06-30
- 项目状态:未结题
- 来源:
- 关键词:AnimalsAntibioticsBacillus subtilisBacteriaBacterial PhysiologyBehaviorBiological ModelsCell CommunicationCellsChemicalsCommunitiesComplexCuesDevelopmentEcologyEnvironmentGeneticGenetic TranscriptionHeterogeneityInterventionMetabolicMetabolismMicrobeMicrobial BiofilmsMolecularNational Institute of General Medical SciencesNaturePhylogenetic AnalysisPhysiologyPlanetsPlantsPlayPrevalenceProbioticsResearchRoleShapesSignal TransductionSoilSourceSpatial DistributionSpecificitySwimmingSystemTherapeuticbacterial communityextracellularinterestmembermicrobialmicrobial communitymicrobiomesecondary metabolitetool
项目摘要
PROJECT SUMMARY
In nature, bacteria typically exist within multispecies communities. Bacterial communities play vital roles in
shaping the environment and their plant and animals. In spite of the prevalence and importance of microbial
communities, important gaps still remain in our understanding of how bacteria interact within these microbiomes.
Our lab’s NIGMS-relevant research is focused on investigating the chemical, molecular, and genetic
mechanisms bacteria use to chemically and physically interact within multispecies communities. We focus on
the soil microbiome as our model system: the soil is not one of the most phylogenetically diverse microbial
environments on the planet, but soil microbes are also the source of the majority of our antibiotics and many
other therapeutics. Therefore, understanding the mechanisms bacteria use to interact within this natural
environment will not only provide a systems-level understanding of complex natural microbiome interactions but
also provide us with potential chemical tools and therapeutic leads to manipulate bacterial behavior. These
bacterially generated compounds are celled specialized or secondary metabolites. These secreted chemical
cues can act as cell-cell communication signals that influence the physiology and metabolism of neighboring
bacteria. They play key roles in bacterial differentiation, or the development of transcriptionally distinct,
heterogeneously expressed subpopulations of cells. We focus on the soil and probiotic bacterium Bacillus
subtilis, which can differentiate into cells that are making biofilm matrix, swimming, or sporulating, among others.
We are interested in understanding the transcriptional specificity, ancestral lineages, and spatial distributions of
this cellular heterogeneity as well as what roles specialized metabolites play in its development. We also aim to
discover specialized metabolites involved in interspecies cell-cell communication to expand our understanding
of chemical interactions in native microbiomes and obtain chemical tools to modulate bacterial physiology.
Finally, we seek to identify the genetic and molecular mechanisms these extracellular signals use to impact
bacterial transcription, heterogeneity, and metabolic activity. This research is significant because it will reveal
fundamental information about the chemical and genetic mechanisms bacteria use to interact with one another.
Our results will deepen our molecular understanding of cell-cell interactions within microbial communities as well
as enable the development of targeted interventions to manipulate microbial behavior in environmentally and
therapeutically important bacteria.
项目摘要
在自然界中,细菌通常存在于多物种群落中。细菌群落在以下方面发挥着重要作用:
塑造环境和动植物。尽管微生物的流行和重要性
然而,在我们对细菌如何在这些微生物群中相互作用的理解方面,仍然存在重要的差距。
我们实验室的NIGMS相关研究集中在研究化学,分子和遗传
细菌在多物种群落中进行化学和物理相互作用的机制。我们专注于
土壤微生物组作为我们的模型系统:土壤不是最具遗传多样性的微生物之一,
地球上的环境,但土壤微生物也是我们大多数抗生素的来源,
其他疗法。因此,了解细菌在这种自然环境中相互作用的机制,
环境不仅可以提供对复杂的自然微生物组相互作用的系统级理解,
也为我们提供了潜在的化学工具和治疗线索来操纵细菌的行为。这些
细菌产生的化合物是细胞特化的或次级代谢物。这些分泌的化学物质
线索可以作为细胞间的通信信号,影响邻近细胞的生理和代谢。
细菌它们在细菌分化或转录上不同的,
异质表达的细胞亚群。我们专注于土壤和益生菌芽孢杆菌
枯草芽孢杆菌,其可以分化成制造生物膜基质、游动或孢子形成等的细胞。
我们感兴趣的是了解转录特异性,祖先谱系和空间分布,
这种细胞的异质性以及特殊的代谢物在其发展中发挥的作用。我们还旨在
发现参与物种间细胞间通讯的特殊代谢物,以扩大我们的理解
研究天然微生物组中的化学相互作用,并获得调节细菌生理学的化学工具。
最后,我们试图确定这些细胞外信号用于影响的遗传和分子机制,
细菌转录、异质性和代谢活性。这项研究意义重大,因为它将揭示
关于细菌相互作用的化学和遗传机制的基本信息。
我们的研究结果也将加深我们对微生物群落中细胞-细胞相互作用的分子理解
能够开发有针对性的干预措施来操纵环境中的微生物行为,
重要的治疗细菌。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Elizabeth Anne Shank其他文献
Elizabeth Anne Shank的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Elizabeth Anne Shank', 18)}}的其他基金
Investigating the molecules and mechanisms of bacterial cell-cell interactions
研究细菌细胞间相互作用的分子和机制
- 批准号:
10406576 - 财政年份:2022
- 资助金额:
$ 41.88万 - 项目类别:
Investigating the molecules and mechanisms of bacterial cell-cell interactions
研究细菌细胞间相互作用的分子和机制
- 批准号:
10818959 - 财政年份:2022
- 资助金额:
$ 41.88万 - 项目类别:
Investigating the molecules and mechanisms of bacterial cell-cell interactions
研究细菌细胞间相互作用的分子和机制
- 批准号:
10798910 - 财政年份:2022
- 资助金额:
$ 41.88万 - 项目类别:
Using Co-culture and Bioinformatics to Discover New Antibiotic Bioactivities
利用共培养和生物信息学发现新的抗生素生物活性
- 批准号:
10056652 - 财政年份:2016
- 资助金额:
$ 41.88万 - 项目类别:
相似海外基金
Can antibiotics disrupt biogeochemical nitrogen cycling in the coastal ocean?
抗生素会破坏沿海海洋的生物地球化学氮循环吗?
- 批准号:
2902098 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Studentship
The role of RNA repair in bacterial responses to translation-inhibiting antibiotics
RNA修复在细菌对翻译抑制抗生素的反应中的作用
- 批准号:
BB/Y004035/1 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Research Grant
Metallo-Peptides: Arming Cyclic Peptide Antibiotics with New Weapons to Combat Antimicrobial Resistance
金属肽:用新武器武装环肽抗生素以对抗抗菌素耐药性
- 批准号:
EP/Z533026/1 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Research Grant
Towards the sustainable discovery and development of new antibiotics
迈向新抗生素的可持续发现和开发
- 批准号:
FT230100468 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
ARC Future Fellowships
DYNBIOTICS - Understanding the dynamics of antibiotics transport in individual bacteria
DYNBIOTICS - 了解抗生素在单个细菌中转运的动态
- 批准号:
EP/Y023528/1 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Research Grant
Engineering Streptomyces bacteria for the sustainable manufacture of antibiotics
工程化链霉菌用于抗生素的可持续生产
- 批准号:
BB/Y007611/1 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Research Grant
The disulfide bond as a chemical tool in cyclic peptide antibiotics: engineering disulfide polymyxins and murepavadin
二硫键作为环肽抗生素的化学工具:工程化二硫多粘菌素和 murepavadin
- 批准号:
MR/Y033809/1 - 财政年份:2024
- 资助金额:
$ 41.88万 - 项目类别:
Research Grant
Role of phenotypic heterogeneity in mycobacterial persistence to antibiotics: Prospects for more effective treatment regimens
表型异质性在分枝杆菌对抗生素持久性中的作用:更有效治疗方案的前景
- 批准号:
494853 - 财政年份:2023
- 资助金额:
$ 41.88万 - 项目类别:
Operating Grants
Imbalance between cell biomass production and envelope biosynthesis underpins the bactericidal activity of cell wall -targeting antibiotics
细胞生物量产生和包膜生物合成之间的不平衡是细胞壁靶向抗生素杀菌活性的基础
- 批准号:
2884862 - 财政年份:2023
- 资助金额:
$ 41.88万 - 项目类别:
Studentship
Narrow spectrum antibiotics for the prevention and treatment of soft-rot plant disease
防治植物软腐病的窄谱抗生素
- 批准号:
2904356 - 财政年份:2023
- 资助金额:
$ 41.88万 - 项目类别:
Studentship