ALS Efficiently Networking Advanced Beam Line Experiments (ALS-ENABLE)

ALS 高效联网高级光束线实验 (ALS-ENABLE)

• 批准号: 10798865
• 负责人: PAUL David ADAMS
• 金额: $ 43.21万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798865
• 关键词: Biological; California; Communities; Development; Goals; Health; Human; Life; Light; Mass Spectrum Analysis; Methods; Performance; Pharmaceutical Preparations; Research; Resources; Roentgen Rays; Route; Source; Structure; Synchrotrons; Techniques; Technology; Training; Work; X-Ray Crystallography; beamline; biological systems; experience; experimental study; human disease; improved; member; novel therapeutics; response; structural biology; tool

Overall Summary/Abstract The overall goal of this proposal is to continue to provide an integrated, efficient synchrotron structural biology Resource to the research community. This Resource, called ALS-ENABLE, is located at the Advanced Light Source (ALS) in Berkeley, California. The team has two decades of experience operating macromolecular X-ray crystallography (MX) and small angle X-ray scattering (SAXS) beamlines, and more recently, an X-ray footprinting mass spectrometry (XFMS) beamline. The team has worked closely together over the last 4 years to create the ALS-ENABLE Resource, and many of the team members are cross-trained in the three X-ray structural biology methods. During the 4 years we have implemented a transparent interface to the ALS structural biology resources, and helped users pursue successful structure determination for both routine and challenging problems. We have worked with a diverse user community, ranging from experts to new synchrotron users with limited training in structural biology techniques. Where necessary we have guided users through the most appropriate routes for answering their biological question. In this renewal application we propose to make several changes to the Resource in response to recent changes in the field of structural biology, leverage a new high- performance beamline (GEMINI), and incorporate the now mature synchrotron technique of X-ray footprinting (XFMS).

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