ALS Efficiently Networking Advanced Beam Line Experiments (ALS-ENABLE)

ALS 高效联网高级光束线实验 (ALS-ENABLE)

• 批准号: 10798865
• 负责人: PAUL David ADAMS
• 金额: $ 43.21万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798865
• 关键词: Biological; California; Communities; Development; Goals; Health; Human; Life; Light; Mass Spectrum Analysis; Methods; Performance; Pharmaceutical Preparations; Research; Resources; Roentgen Rays; Route; Source; Structure; Synchrotrons; Techniques; Technology; Training; Work; X-Ray Crystallography; beamline; biological systems; experience; experimental study; human disease; improved; member; novel therapeutics; response; structural biology; tool

Overall Summary/Abstract The overall goal of this proposal is to continue to provide an integrated, efficient synchrotron structural biology Resource to the research community. This Resource, called ALS-ENABLE, is located at the Advanced Light Source (ALS) in Berkeley, California. The team has two decades of experience operating macromolecular X-ray crystallography (MX) and small angle X-ray scattering (SAXS) beamlines, and more recently, an X-ray footprinting mass spectrometry (XFMS) beamline. The team has worked closely together over the last 4 years to create the ALS-ENABLE Resource, and many of the team members are cross-trained in the three X-ray structural biology methods. During the 4 years we have implemented a transparent interface to the ALS structural biology resources, and helped users pursue successful structure determination for both routine and challenging problems. We have worked with a diverse user community, ranging from experts to new synchrotron users with limited training in structural biology techniques. Where necessary we have guided users through the most appropriate routes for answering their biological question. In this renewal application we propose to make several changes to the Resource in response to recent changes in the field of structural biology, leverage a new high- performance beamline (GEMINI), and incorporate the now mature synchrotron technique of X-ray footprinting (XFMS).

摘要/Abstract 这项建议的总体目标是继续提供一个综合的，高效的同步加速器结构生物学 研究界的资源。此资源称为ALS-ENABLE，位于Advanced Light 来源（ALS）在伯克利，加州.该团队拥有20年的大分子X射线操作经验 晶体学（MX）和小角X射线散射（SAXS）光束线，以及最近的X射线衍射（XRD）。 足迹质谱（XFMS）光束线。该团队在过去4年中密切合作， 创建ALS-ENABLE资源，许多团队成员在三个X射线方面进行了交叉培训 结构生物学方法在4年的时间里，我们实现了与ALS结构的透明接口， 生物资源，并帮助用户追求成功的结构测定，无论是常规的和具有挑战性的 问题我们与各种各样的用户社区合作，从专家到新的同步加速器用户， 有限的结构生物学技术培训。必要时，我们会引导用户完成最重要的操作 回答他们生物学问题的合适途径。在这一更新申请中，我们提出了几个 为响应结构生物学领域的最新变化，对资源进行了修改，利用了一个新的高度- 高性能光束线（GEMINI），并结合目前成熟的X射线足迹同步加速器技术 （XFMS）。