EIGER2 S 16M Detector

EIGER2 S 16M 探测器

• 批准号: 10415587
• 负责人: PAUL David ADAMS
• 金额: $ 182.09万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415587
• 关键词: Address; Area; Automation; Cells; Collection; Complement; Computer software; Crystallography; Data; Data Collection; Development; Funding; Future; Goals; Gold; Light; Modernization; Noise; Performance; Photons; Radiation induced damage; Research Personnel; Resolution; Resources; Sampling; Slice; Source; Speed; Surface; Synchrotrons; System; Techniques; Temperature; United States; United States National Institutes of Health; base; beamline; design; detector; experimental study; operation; quantum; structural biology; success

Project Summary/Abstract The goal of this proposal is the purchase and installation of a high-performance DECTRIS EIGER2 S 16M pixel array detector for the new microfocus branchline of GEMINI at the Advanced Light Source (ALS). The Berkeley Center for Structural Biology (BCSB) operates six macromolecular crystallography beamlines at the ALS. Its latest undulator-based microfocus beamline, GEMINI, is just starting operation and available to NIH users. BCSB has received funds for the construction of a second microfocus beamline from the same source. Building a second branch that provides a high brightness user-focusable beam down to 15 microns is required to address the national demand for high brightness small-beam crystallography in the United States where existing microfocus beamlines are oversubscribed and demand far exceeds capacity. Addressing this lack of national capacity now is particularly critical, as we prepare for half of the total U.S. synchrotron macromolecular crystallography resources going dark in the near future with the upcoming major upgrade of the Advanced Photon Source and the enormous impact that will have on NIH-funded investigators. Guided by the expertise of the BCSB development team, our beamlines provide state-of-the-art capabilities in automation, software processing, and hardware control; the addition of a large surface area, fast-readout, high-end detector system at GEMINI will complement the qualities of the new GEMINI beamline and allow further development of new techniques in multi- temperature crystallography and synchrotron serial crystallography. Photon counting pixel array detectors are the gold standard in macromolecular crystallography due to their zero noise, high frame rate, excellent point-spread function, and high dynamic range; they have become standard at many national synchrotron facilities throughout the world. These detectors have been critical in the success of many structural biology projects and the latest design by DECTRIS, the EIGER2, have marked improvements on the speed and quantum efficiency of detectors that are available at the ALS. The EIGER2 S 16M has zero deadtime due to its two counters per pixel that enables fine slicing data collection that can reduce radiation damage—especially in multi-temperature experiments. The high dynamic range means both strong and weak reflections can be collected simultaneously, and the large surface area combined with a single pixel point-spread-function allows collection on samples with large unit cells and/or high resolution. These attributes will maximize the success of NIH researchers at GEMINI and allow the BCSB to excel in its commitment to provide our users the highest quality data and the cutting- edge capabilities needed at the modern synchrotron.

项目摘要/摘要 该提案的目的是购买和安装高性能Dectris eiger2 s 高级光源的新型双子座分支线的16m像素阵列检测器 （ALS）。伯克利结构生物学中心（BCSB）运营六个大分子晶体学 ALS的梁线。它的最新基于解开器的微型焦点双线线Gemini刚刚开始操作 并向NIH用户使用。 BCSB已收到用于构建第二微型对象的资金 来自同一来源的梁线。建立第二个分支，可提供高亮度用户可用的 需要向下降至15微米以满足国家对高亮度小束的需求 在美国，现有的微聚焦束线的晶体学超过订阅，并且 需求远远超过容量。现在解决这种缺乏国家能力特别关键，因为我们 准备美国同步总分的一半大分子晶体学资源在黑暗中变黑 随着即将进行的高级光子源的重大升级和巨大影响 这将是NIH资助的调查人员。在BCSB开发团队的专业知识的指导下，我们的 梁线在自动化，软件处理和硬件控制方面提供最先进的功能； Gemini的大型表面积，快速阅读的高端检测器系统将完成 新的双子座光束线的素质，并允许在多种技术中进一步开发新技术 温度晶体学和同步基串行晶体学。光子计数像素阵列 探测器是大分子晶体学的金标准，由于其零噪声，高框架 速率，出色的点传播功能和高动态范围；他们已经成为许多标准 全球国家同步设施。这些探测器对于成功的成功至关重要 许多结构生物学项目和eiger2的Dectris的最新设计已标记 ALS可用的检测器的速度和量子效率的提高。这 Eiger2 S 16m的死时间为零，因为每个像素的两个计数器可实现精细的切片数据收集 这可以减少辐射损伤，尤其是在多温度实验中。高动态范围 意味着可以简单收集强反射和弱反射 结合单个像素点传播功能，可以收集具有大型单元的样品 和/或高分辨率。这些属性将最大化Demini的NIH研究人员的成功 允许BCSB擅长为我们的用户提供最高质量的数据和削减 - 现代同步器需要的边缘功能。