EIGER2 S 16M Detector

EIGER2 S 16M 探测器

• 批准号: 10415587
• 负责人: PAUL David ADAMS
• 金额: $ 182.09万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415587
• 关键词: Address; Area; Automation; Cells; Collection; Complement; Computer software; Crystallography; Data; Data Collection; Development; Funding; Future; Goals; Gold; Light; Modernization; Noise; Performance; Photons; Radiation induced damage; Research Personnel; Resolution; Resources; Sampling; Slice; Source; Speed; Surface; Synchrotrons; System; Techniques; Temperature; United States; United States National Institutes of Health; base; beamline; design; detector; experimental study; operation; quantum; structural biology; success

Project Summary/Abstract The goal of this proposal is the purchase and installation of a high-performance DECTRIS EIGER2 S 16M pixel array detector for the new microfocus branchline of GEMINI at the Advanced Light Source (ALS). The Berkeley Center for Structural Biology (BCSB) operates six macromolecular crystallography beamlines at the ALS. Its latest undulator-based microfocus beamline, GEMINI, is just starting operation and available to NIH users. BCSB has received funds for the construction of a second microfocus beamline from the same source. Building a second branch that provides a high brightness user-focusable beam down to 15 microns is required to address the national demand for high brightness small-beam crystallography in the United States where existing microfocus beamlines are oversubscribed and demand far exceeds capacity. Addressing this lack of national capacity now is particularly critical, as we prepare for half of the total U.S. synchrotron macromolecular crystallography resources going dark in the near future with the upcoming major upgrade of the Advanced Photon Source and the enormous impact that will have on NIH-funded investigators. Guided by the expertise of the BCSB development team, our beamlines provide state-of-the-art capabilities in automation, software processing, and hardware control; the addition of a large surface area, fast-readout, high-end detector system at GEMINI will complement the qualities of the new GEMINI beamline and allow further development of new techniques in multi- temperature crystallography and synchrotron serial crystallography. Photon counting pixel array detectors are the gold standard in macromolecular crystallography due to their zero noise, high frame rate, excellent point-spread function, and high dynamic range; they have become standard at many national synchrotron facilities throughout the world. These detectors have been critical in the success of many structural biology projects and the latest design by DECTRIS, the EIGER2, have marked improvements on the speed and quantum efficiency of detectors that are available at the ALS. The EIGER2 S 16M has zero deadtime due to its two counters per pixel that enables fine slicing data collection that can reduce radiation damage—especially in multi-temperature experiments. The high dynamic range means both strong and weak reflections can be collected simultaneously, and the large surface area combined with a single pixel point-spread-function allows collection on samples with large unit cells and/or high resolution. These attributes will maximize the success of NIH researchers at GEMINI and allow the BCSB to excel in its commitment to provide our users the highest quality data and the cutting- edge capabilities needed at the modern synchrotron.

项目概要/摘要 该提案的目标是购买和安装高性能 DECTRIS EIGER2 S 1600 万像素阵列探测器，用于高级光源处 GEMINI 的新型微焦点分支线 （肌萎缩侧索硬化症）。伯克利结构生物学中心 (BCSB) 运营着六项大分子晶体学研究 ALS 的光束线。其最新的基于波荡器的微焦点光束线 GEMINI 刚刚开始运行 并可供 NIH 用户使用。 BCSB 已收到用于建造第二个微焦点的资金 来自同一来源的光束线。构建第二个分支，提供高亮度用户可聚焦 光束小至15微米以满足国家对高亮度小光束的需求 在美国的晶体学领域，现有的微焦点光束线已被超额认购， 需求远远超过容量。现在解决国家能力不足的问题尤为重要，因为我们 为美国同步加速器高分子晶体学资源总量的一半陷入困境做好准备 不久的将来，先进光子源即将进行重大升级，带来巨大影响 这将对 NIH 资助的研究人员产生影响。在 BCSB 开发团队的专业知识指导下，我们的 光束线提供最先进的自动化、软件处理和硬件控制功能； GEMINI 增加的大表面积、快速读出的高端探测器系统将补充 新 GEMINI 光束线的质量，并允许进一步开发多方面的新技术 温度晶体学和同步加速器系列晶体学。光子计数像素阵列 探测器因其零噪声、高帧率而成为高分子晶体学的黄金标准 率、优异的点扩散功能、高动态范围；它们已成为许多地方的标准 世界各地的国家同步加速器设施。这些探测器对成功至关重要 许多结构生物学项目和 DECTRIS 的最新设计 EIGER2 已标志着 改进了 ALS 可用探测器的速度和量子效率。这 EIGER2 S 16M 具有零死区时间，因为每个像素有两个计数器，可实现精细切片数据收集 可以减少辐射损伤——尤其是在多温度实验中。高动态范围 意味着可以同时收集强反射和弱反射，并且表面积大 与单像素点扩散函数相结合，可以收集具有大单位单元的样本 和/或高分辨率。这些特性将最大限度地提高 NIH 研究人员在 GEMINI 和 让 BCSB 能够出色地履行其承诺，为我们的用户提供最高质量的数据和切割- 现代同步加速器所需的边缘能力。