EIGER2 S 16M Detector

EIGER2 S 16M 探测器

• 批准号: 10415587
• 负责人: PAUL David ADAMS
• 金额: $ 182.09万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415587
• 关键词: Address; Area; Automation; Cells; Collection; Complement; Computer software; Crystallography; Data; Data Collection; Development; Funding; Future; Goals; Gold; Light; Modernization; Noise; Performance; Photons; Radiation induced damage; Research Personnel; Resolution; Resources; Sampling; Slice; Source; Speed; Surface; Synchrotrons; System; Techniques; Temperature; United States; United States National Institutes of Health; base; beamline; design; detector; experimental study; operation; quantum; structural biology; success

Project Summary/Abstract The goal of this proposal is the purchase and installation of a high-performance DECTRIS EIGER2 S 16M pixel array detector for the new microfocus branchline of GEMINI at the Advanced Light Source (ALS). The Berkeley Center for Structural Biology (BCSB) operates six macromolecular crystallography beamlines at the ALS. Its latest undulator-based microfocus beamline, GEMINI, is just starting operation and available to NIH users. BCSB has received funds for the construction of a second microfocus beamline from the same source. Building a second branch that provides a high brightness user-focusable beam down to 15 microns is required to address the national demand for high brightness small-beam crystallography in the United States where existing microfocus beamlines are oversubscribed and demand far exceeds capacity. Addressing this lack of national capacity now is particularly critical, as we prepare for half of the total U.S. synchrotron macromolecular crystallography resources going dark in the near future with the upcoming major upgrade of the Advanced Photon Source and the enormous impact that will have on NIH-funded investigators. Guided by the expertise of the BCSB development team, our beamlines provide state-of-the-art capabilities in automation, software processing, and hardware control; the addition of a large surface area, fast-readout, high-end detector system at GEMINI will complement the qualities of the new GEMINI beamline and allow further development of new techniques in multi- temperature crystallography and synchrotron serial crystallography. Photon counting pixel array detectors are the gold standard in macromolecular crystallography due to their zero noise, high frame rate, excellent point-spread function, and high dynamic range; they have become standard at many national synchrotron facilities throughout the world. These detectors have been critical in the success of many structural biology projects and the latest design by DECTRIS, the EIGER2, have marked improvements on the speed and quantum efficiency of detectors that are available at the ALS. The EIGER2 S 16M has zero deadtime due to its two counters per pixel that enables fine slicing data collection that can reduce radiation damage—especially in multi-temperature experiments. The high dynamic range means both strong and weak reflections can be collected simultaneously, and the large surface area combined with a single pixel point-spread-function allows collection on samples with large unit cells and/or high resolution. These attributes will maximize the success of NIH researchers at GEMINI and allow the BCSB to excel in its commitment to provide our users the highest quality data and the cutting- edge capabilities needed at the modern synchrotron.

项目摘要/摘要 该提案的目标是购买并安装一台高性能的DECTRIS EIGER2 S 用于先进光源双子座新微焦支线的16M像素阵列探测器 (ALS)。伯克利结构生物学中心(BCSB)运行着六个大分子结晶学 肌萎缩侧索硬化症的光束线。其最新的基于波荡器的微焦光束线双子座刚刚开始运行 并可供NIH用户使用。BCSB已收到建造第二个微焦点的资金 来自同一来源的光束线。构建第二个分支，提供高亮度用户可聚焦 为了满足国家对高亮度小光束的需求，需要将光束细化到15微米 美国的结晶学，现有的微焦光束线被超额认购， 需求远远超过了产能。解决这一国家能力不足的问题现在尤为关键，因为我们 为美国同步加速器大分子晶体资源总量的一半做好准备 在不久的将来，高级光子源即将进行重大升级，并带来巨大的影响 这将对NIH资助的调查人员产生影响。在BCSB开发团队的专业知识指导下，我们的 光束线在自动化、软件处理和硬件控制方面提供最先进的能力； 在双子座增加一个大表面积、快速读出的高端探测器系统将是补充 新的双子座光束线的质量，并允许进一步开发新技术在多个 温度结晶学和同步加速器系列结晶学。光子计数像元阵列 探测器具有零噪声、高分辨率等优点，是大分子结晶学的金标准。 速率、出色的点扩展函数和高动态范围；它们在许多方面都已成为标准 世界各地的国家同步加速器设施。这些探测器在成功的 许多结构生物学项目和DECTRIS的最新设计EIGER2已经标志着 在ALS提供的探测器的速度和量子效率方面的改进。这个 图2 S 16M的死区时间为零，因为它的每个像素有两个计数器，可以进行精细切片数据收集 这可以减少辐射损害--特别是在多温度实验中。高动态范围 意味着可以同时收集强反射和弱反射，并且大的表面积 与单像素点扩散函数相结合，允许对具有大单位像元的样本进行采集 和/或高分辨率。这些特性将使NIH研究人员在双子座和 让BCSB履行其向用户提供最高质量数据和切割的承诺- 现代同步加速器所需的尖端能力。