EIGER2 S 16M Detector

项目概要/摘要 本提案的目标是购买和安装高性能DECTRIS EIGER 2 S 1600万像素阵列探测器，用于先进光源GEMINI的新微焦点分支线 （ALS）。伯克利结构生物学中心（BCSB）操作六个大分子晶体学 ALS的光束线其最新的基于波动器的微聚焦光束线GEMINI刚刚开始运行 并提供给NIH用户。BCSB已收到建造第二个微焦点的资金 来自同一个源的光束线。构建第二分支，其提供高亮度的用户可聚焦的 为了满足国家对高亮度小光束的需求，需要将光束缩小到15微米 美国的晶体学，现有的微焦点光束线已超额认购， 需求远远超过能力。现在解决国家能力不足的问题特别重要，因为我们 准备一半的美国总同步加速器大分子晶体学资源在黑暗中 在不久的将来，随着先进光子源的重大升级和巨大的影响， 这将对NIH资助的研究人员产生影响。在BCSB开发团队的专业知识指导下，我们的 光束线在自动化、软件处理和硬件控制方面具有最先进的能力; 在GEMINI增加一个大表面积、快速读出、高端探测器系统将补充 新的GEMINI光束线的质量，并允许进一步开发新技术， 温度晶体学和同步加速器系列晶体学。光子计数像素阵列 探测器是大分子晶体学中的黄金标准，因为它们具有零噪声、高帧 速率、出色的点扩展功能和高动态范围;它们已成为许多 国家同步加速器设施遍布世界各地。这些探测器在成功的 许多结构生物学项目和DECTRIS的最新设计EIGER 2，标志着 改进ALS现有探测器的速度和量子效率。的 EIGER 2 S 16 M的每个像素有两个计数器，因此具有零死区时间，可实现精细切片数据收集 这可以减少辐射损伤-特别是在多温度实验中。高动态范围 这意味着可以同时收集强反射和弱反射， 与单个像素点扩散函数相结合，允许对具有大单位单元的样本进行收集 和/或高分辨率。这些属性将使GEMINI的NIH研究人员的成功最大化， 允许BCSB在其承诺中脱颖而出，为我们的用户提供最高质量的数据和切割- 现代同步加速器所需的尖端能力。

Project Summary/Abstract The goal of this proposal is the purchase and installation of a high-performance DECTRIS EIGER2 S 16M pixel array detector for the new microfocus branchline of GEMINI at the Advanced Light Source (ALS). The Berkeley Center for Structural Biology (BCSB) operates six macromolecular crystallography beamlines at the ALS. Its latest undulator-based microfocus beamline, GEMINI, is just starting operation and available to NIH users. BCSB has received funds for the construction of a second microfocus beamline from the same source. Building a second branch that provides a high brightness user-focusable beam down to 15 microns is required to address the national demand for high brightness small-beam crystallography in the United States where existing microfocus beamlines are oversubscribed and demand far exceeds capacity. Addressing this lack of national capacity now is particularly critical, as we prepare for half of the total U.S. synchrotron macromolecular crystallography resources going dark in the near future with the upcoming major upgrade of the Advanced Photon Source and the enormous impact that will have on NIH-funded investigators. Guided by the expertise of the BCSB development team, our beamlines provide state-of-the-art capabilities in automation, software processing, and hardware control; the addition of a large surface area, fast-readout, high-end detector system at GEMINI will complement the qualities of the new GEMINI beamline and allow further development of new techniques in multi- temperature crystallography and synchrotron serial crystallography. Photon counting pixel array detectors are the gold standard in macromolecular crystallography due to their zero noise, high frame rate, excellent point-spread function, and high dynamic range; they have become standard at many national synchrotron facilities throughout the world. These detectors have been critical in the success of many structural biology projects and the latest design by DECTRIS, the EIGER2, have marked improvements on the speed and quantum efficiency of detectors that are available at the ALS. The EIGER2 S 16M has zero deadtime due to its two counters per pixel that enables fine slicing data collection that can reduce radiation damage—especially in multi-temperature experiments. The high dynamic range means both strong and weak reflections can be collected simultaneously, and the large surface area combined with a single pixel point-spread-function allows collection on samples with large unit cells and/or high resolution. These attributes will maximize the success of NIH researchers at GEMINI and allow the BCSB to excel in its commitment to provide our users the highest quality data and the cutting- edge capabilities needed at the modern synchrotron.