Causes and consequences of interpersonal microbial variation

人际微生物变异的原因和后果

• 批准号: 10798726
• 负责人: Andrew L Goodman
• 金额: $ 25万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798726
• 关键词: Aging; Diet; Equipment; Funding; Germ-Free; Gnotobiotic; Goals; Health; Human Biology; Maintenance; Mass Spectrum Analysis; Measures; Metabolism; Microbial Genetics; Molecular; National Institute of General Medical Sciences; Preparation; Process; Research; Research Project Grants; Shapes; Site; Time; Universities; Variant; Work; access restrictions; cost; dietary; disorder risk; gut microbiome; gut microbiota; instrument; mass spectrometer; microbial; microbiome; mouse model; parent grant; parent project; treatment response

Summary The purpose of this proposal is to provide equipment support to our currently funded NIGMS research project (2R35GM118159-08). Despite the possibility that the gut microbiome may represent a critical and readily modifiable component of human biology, the contribution of the gut microbiota to health, disease risk, and response to therapy remains largely undefined. The overall goal of the parent grant is to understand the principles, mechanisms, and processes that shape the interaction between gut microbial communities and their hosts. Our strategy is to combine anaerobic microbial genetics and high-throughput mass spectrometry with gnotobiotic (germfree and ex-germfree) mouse models to dissect these interactions. The central analytical approach in these studies is mass spectrometry, which we use to precisely measure microbial metabolism of dietary and other compounds and identify metabolites produced as a result of these activities. Although we have made significant progress in the project, the main limiting factor is the restricted access to time on a mass spectrometer. For this reason, we request funds to partially support the purchase of a mass spectrometer to support this project. The purchase cost, site preparation, installation, and maintenance of this instrument will also be significantly supported by the university.

摘要 这项建议的目的是为我们目前资助的NIGMS研究提供设备支持 项目(2R35GM118159-08)。尽管肠道微生物群可能代表着一种关键的 和易于修改的人类生物学组成部分，肠道微生物区系对健康的贡献， 疾病风险和对治疗的反应在很大程度上仍然不确定。家长助学金的总体目标 是了解塑造肠道之间相互作用的原理、机制和过程 微生物群落及其宿主。我们的战略是将厌氧微生物遗传学和 GnotoBiotic(无菌和无菌)小鼠模型的高通量质谱仪 剖析这些互动。这些研究中的中心分析方法是质谱学， 我们用它来精确测量饮食和其他化合物的微生物代谢，并确定 代谢产物由这些活动产生的代谢物尽管我们在以下方面取得了重大进展 在该项目中，主要的限制因素是在质谱仪上获取时间的限制。为了这个 原因是，我们请求资金部分支持购买质谱仪来支持这一点 项目。该仪器的采购成本、现场准备、安装和维护也将 得到大学的大力支持。

项目成果

Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.

• DOI: 10.1016/j.cell.2017.03.045
• 发表时间: 2017-04-20
• 期刊: Cell
• 影响因子: 64.5
• 作者: Lim B;Zimmermann M;Barry NA;Goodman AL
• 通讯作者: Goodman AL

Gut microbes modulate (p)ppGpp during a time-restricted feeding regimen.

• DOI: 10.1128/mbio.01907-23
• 发表时间: 2023-12-19
• 期刊: mBio
• 影响因子: 6.4

A Master Regulator of Bacteroides thetaiotaomicron Gut Colonization Controls Carbohydrate Utilization and an Alternative Protein Synthesis Factor.

多形拟杆菌肠道定植的主要调节因子控制碳水化合物的利用和替代蛋白质合成因子。

• DOI: 10.1128/mbio.03221-19
• 发表时间: 2020
• 期刊: mBio
• 影响因子: 6.4
• 作者: Townsend2nd,GuyE;Han,Weiwei;Schwalm3rd,NathanD;Hong,Xinyu;Bencivenga-Barry,NatashaA;Goodman,AndrewL;Groisman,EduardoA
• 通讯作者: Groisman,EduardoA

An insider's perspective: Bacteroides as a window into the microbiome.

• DOI: 10.1038/nmicrobiol.2017.26
• 发表时间: 2017-04-25
• 期刊: Nature microbiology
• 影响因子: 28.3
• 作者: Wexler AG;Goodman AL
• 通讯作者: Goodman AL

A Common Pathway for Activation of Host-Targeting and Bacteria-Targeting Toxins in Human Intestinal Bacteria.

• DOI: 10.1128/mbio.00656-21
• 发表时间: 2021-08-31
• 期刊: mBio
• 影响因子: 6.4
• 作者: Bao Y;Verdegaal AA;Anderson BW;Barry NA;He J;Gao X;Goodman AL
• 通讯作者: Goodman AL