Causes and consequences of interpersonal microbial variation

人际微生物变异的原因和后果

• 批准号: 10798726
• 负责人: Andrew L Goodman
• 金额: $ 25万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798726
• 关键词: Aging; Diet; Equipment; Funding; Germ-Free; Gnotobiotic; Goals; Health; Human Biology; Maintenance; Mass Spectrum Analysis; Measures; Metabolism; Microbial Genetics; Molecular; National Institute of General Medical Sciences; Preparation; Process; Research; Research Project Grants; Shapes; Site; Time; Universities; Variant; Work; access restrictions; cost; dietary; disorder risk; gut microbiome; gut microbiota; instrument; mass spectrometer; microbial; microbiome; mouse model; parent grant; parent project; treatment response

Summary The purpose of this proposal is to provide equipment support to our currently funded NIGMS research project (2R35GM118159-08). Despite the possibility that the gut microbiome may represent a critical and readily modifiable component of human biology, the contribution of the gut microbiota to health, disease risk, and response to therapy remains largely undefined. The overall goal of the parent grant is to understand the principles, mechanisms, and processes that shape the interaction between gut microbial communities and their hosts. Our strategy is to combine anaerobic microbial genetics and high-throughput mass spectrometry with gnotobiotic (germfree and ex-germfree) mouse models to dissect these interactions. The central analytical approach in these studies is mass spectrometry, which we use to precisely measure microbial metabolism of dietary and other compounds and identify metabolites produced as a result of these activities. Although we have made significant progress in the project, the main limiting factor is the restricted access to time on a mass spectrometer. For this reason, we request funds to partially support the purchase of a mass spectrometer to support this project. The purchase cost, site preparation, installation, and maintenance of this instrument will also be significantly supported by the university.

总结 这项建议的目的是为我们目前资助的NIGMS研究提供设备支持 项目（2 R35 GM 118159 -08）。尽管肠道微生物组可能代表了一个关键的 和人类生物学中容易改变的组成部分，肠道微生物群对健康的贡献， 疾病风险和对治疗的反应在很大程度上仍然不确定。父母补助金的总体目标 是了解的原则，机制，和过程，塑造肠道之间的相互作用， 微生物群落及其宿主。我们的策略是将联合收割机厌氧微生物遗传学和 使用无菌（无菌和无菌）小鼠模型进行高通量质谱分析， 剖析这些互动。这些研究中的中心分析方法是质谱法， 我们用它来精确测量饮食和其他化合物的微生物代谢， 由于这些活动而产生的代谢物。虽然我们在这方面取得了重大进展， 就该项目而言，主要的限制因素是质谱仪上的时间有限。为此 因此，我们要求资金部分支持购买质谱仪，以支持这一点。 项目该仪器的购买成本、场地准备、安装和维护也将 得到大学的大力支持。

项目成果

Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.

• DOI: 10.1016/j.cell.2017.03.045
• 发表时间: 2017-04-20
• 期刊: Cell
• 影响因子: 64.5
• 作者: Lim B;Zimmermann M;Barry NA;Goodman AL
• 通讯作者: Goodman AL

Gut microbes modulate (p)ppGpp during a time-restricted feeding regimen.

• DOI: 10.1128/mbio.01907-23
• 发表时间: 2023-12-19
• 期刊: mBio
• 影响因子: 6.4

A Master Regulator of Bacteroides thetaiotaomicron Gut Colonization Controls Carbohydrate Utilization and an Alternative Protein Synthesis Factor.

多形拟杆菌肠道定植的主要调节因子控制碳水化合物的利用和替代蛋白质合成因子。

• DOI: 10.1128/mbio.03221-19
• 发表时间: 2020
• 期刊: mBio
• 影响因子: 6.4
• 作者: Townsend2nd,GuyE;Han,Weiwei;Schwalm3rd,NathanD;Hong,Xinyu;Bencivenga-Barry,NatashaA;Goodman,AndrewL;Groisman,EduardoA
• 通讯作者: Groisman,EduardoA

An insider's perspective: Bacteroides as a window into the microbiome.

• DOI: 10.1038/nmicrobiol.2017.26
• 发表时间: 2017-04-25
• 期刊: Nature microbiology
• 影响因子: 28.3
• 作者: Wexler AG;Goodman AL
• 通讯作者: Goodman AL

A Common Pathway for Activation of Host-Targeting and Bacteria-Targeting Toxins in Human Intestinal Bacteria.

• DOI: 10.1128/mbio.00656-21
• 发表时间: 2021-08-31
• 期刊: mBio
• 影响因子: 6.4
• 作者: Bao Y;Verdegaal AA;Anderson BW;Barry NA;He J;Gao X;Goodman AL
• 通讯作者: Goodman AL