The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

国家原位断层超显微术中心 (NCITU)

• 批准号: 10818768
• 负责人: Jeffrey S Kieft
• 金额: $ 35万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10818768
• 关键词: Cells; Cryo-electron tomography; Electron Microscopy; Equipment; Funding; In Situ; New York; Preparation; Qualifying; Sampling; Services; Specimen; Time; Training; Visualization; Work; computing resources; equipment acquisition; instrument; light microscopy; method development; structural biology; tomography

Project Summary/Abstract The National Center for In-situ Tomographic Ultramicroscopy (NCITU), based at the New York Structural Biology Center, provides user access and service towards preparing specimens for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. We are requesting funds to purchase equipment to upgrade and expand our computational resources and upgrade our Thermo Fisher Scientific (TFS) Aquilos2 for correlative light and electron microscopy (CLEM) work. These upgrades will benefit all users of NCITU by increasing the capability and capacity of these instruments.

项目总结/摘要 位于纽约的国家原位层析超显微镜中心（NCITU） 结构生物学中心，提供用户访问和服务，以准备标本， 大分子缔合和组织的直接可视化，特别是在天然细胞中。 高素质的技术团队提供直接的实践服务，指导和帮助， 确保在最短的时间内以最高的质量制备样品和网格 可能 我们正在申请资金购买设备，以升级和扩大我们的计算能力， 资源和升级我们的赛默飞世尔科技（TFS）Aquilos 2相关的光， 电子显微镜（CLEM）这些升级将使NCITU的所有用户受益， 这些工具的能力和功能。