Surface Plasmon Resonance Instrumentation

项目总结 能够对相互作用进行定量研究的实验技术 不同的生物分子对生物医学研究至关重要。这些技术提供了 重要数据有助于了解多种疾病的分子基础，开发新的发病机制 建立模型，发现新药和治疗方法，并指导其他实验。表面等离子体激元 共振(SPR)已经成为一种值得信赖的、健壮的、多功能的方法来探索 分子结合事件的热力学和动力学。二十年来，SPR仪器 一直是科罗拉多大学生物物理学核心的重要组成部分 Anschutz医学院(CU-AMC)，并已被用于支持NIH资助的各种 项目。然而，20年后，这些设备变得不可靠，维护成本很高， 过时了，很快就不能再修复了。我们建议购买一台新的SPR仪器 为了取代我们目前的工具，确保SPR继续作为我们园区的关键部分 研究基础设施。该仪器将存放在一个既定的、管理良好的和 为生物物理学核心设施提供资金，使其得以维护并与专业知识相结合。 该仪器将由来自多个基础科学和临床科室的研究人员使用 并将是培养下一代生物物理学家和生物化学家的关键部分。 此外，与旧仪器相比，新仪器增强的能力将 允许用更少的材料进行实验，用更多种类的分子， 并且以更高的吞吐量和更高的灵敏度。 收购这一新工具将对美国国立卫生研究院资助的 在传染病、病毒学、癌症、RNA剪接、细胞 蛋白质定位、基因调控、免疫学和药物设计。这些(和 其他)研究领域包括节省时间和物质、更快的数据收集、更高的灵敏度、 能够在更多样化的条件下收集数据，更好的小分子结合检测，以及 更多的培训机会。

PROJECT SUMMARY Experimental techniques that enable quantitative interrogation of the interactions between different biological molecules are critical to biomedical research. Such techniques provide important data to understand the molecular basis of diverse diseases, develop new mechanistic models, discover new drugs and therapies, and guide other experiments. Surface plasmon resonance (SPR) has emerged as a trusted, robust, and versatile method to explore the thermodynamics and kinetics of molecular binding events. For twenty years, SPR instrumentation has been an important and integral part of the Biophysics Core on the University of Colorado Anschutz Medical Campus (CU-AMC) and has been used to support a wide variety of NIH-funded projects. However, after two decades the equipment has become unreliable, costly to maintain, obsolete, and will soon no longer be repairable. We propose to purchase a new SPR instrument to replace our current instrument, ensuring SPR continues as a critical part of our campus’ research infrastructure. The instrument will be housed in an established, well-managed, and funded Biophysics Core facility that will allow it to be maintained and to be coupled with expertise. This instrument will be used by researchers from multiple basic science and clinical departments and will be a key part of training the next generation of biophysicists and biochemists. Furthermore, compared to the old instrument, the new instrument’s enhanced capabilities will allow for experiments to be conducted with much less material, with a greater variety of molecules, and in a higher-throughput way with greater sensitivity. Acquisition of this new instrument will have an immediate and lasting impact on NIH-funded research in areas as diverse as infectious diseases, virology, cancer, RNA splicing, cellular protein localization, gene regulation, immunology, and drug design. The benefits to these (and other) research areas include time and material savings, faster data collection, greater sensitivity, ability to collect data under more diverse conditions, better small molecule binding detection, and enhanced training opportunities.