Surface Plasmon Resonance Instrumentation

表面等离子共振仪器

• 批准号: 10428908
• 负责人: Jeffrey S Kieft
• 金额: $ 20.03万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-17 至 2023-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10428908
• 关键词: Area; Basic Science; Binding; Biological; Biomedical Research; Biophysics; Clinical; Colorado; Communicable Diseases; Core Facility; Coupled; Data; Data Collection; Detection; Disease; Drug Design; Ensure; Equipment; Event; Funding; Gene Expression Regulation; Immunology; Kinetics; Malignant Neoplasms; Medical; Methods; Modeling; Molecular; Pharmacotherapy; Proteins; RNA Splicing; Research; Research Infrastructure; Research Personnel; Savings; Surface Plasmon Resonance; Techniques; Thermodynamics; Time; Training; Trust; United States National Institutes of Health; Universities; cost; experimental study; instrument; instrumentation; next generation; novel therapeutics; repaired; small molecule; training opportunity; virology

PROJECT SUMMARY Experimental techniques that enable quantitative interrogation of the interactions between different biological molecules are critical to biomedical research. Such techniques provide important data to understand the molecular basis of diverse diseases, develop new mechanistic models, discover new drugs and therapies, and guide other experiments. Surface plasmon resonance (SPR) has emerged as a trusted, robust, and versatile method to explore the thermodynamics and kinetics of molecular binding events. For twenty years, SPR instrumentation has been an important and integral part of the Biophysics Core on the University of Colorado Anschutz Medical Campus (CU-AMC) and has been used to support a wide variety of NIH-funded projects. However, after two decades the equipment has become unreliable, costly to maintain, obsolete, and will soon no longer be repairable. We propose to purchase a new SPR instrument to replace our current instrument, ensuring SPR continues as a critical part of our campus’ research infrastructure. The instrument will be housed in an established, well-managed, and funded Biophysics Core facility that will allow it to be maintained and to be coupled with expertise. This instrument will be used by researchers from multiple basic science and clinical departments and will be a key part of training the next generation of biophysicists and biochemists. Furthermore, compared to the old instrument, the new instrument’s enhanced capabilities will allow for experiments to be conducted with much less material, with a greater variety of molecules, and in a higher-throughput way with greater sensitivity. Acquisition of this new instrument will have an immediate and lasting impact on NIH-funded research in areas as diverse as infectious diseases, virology, cancer, RNA splicing, cellular protein localization, gene regulation, immunology, and drug design. The benefits to these (and other) research areas include time and material savings, faster data collection, greater sensitivity, ability to collect data under more diverse conditions, better small molecule binding detection, and enhanced training opportunities.

项目摘要 实验技术可以定量询问之间的相互作用 不同的生物分子对于生物医学研究至关重要。这样的技术提供 重要数据以了解潜水疾病的分子基础，发展新机械 模型，发现新药和疗法，并指导其他实验。表面塑料 共振（SPR）已成为一种可信赖的，强大且多才多艺的方法，可以探索 分子结合事件的热力学和动力学。二十年来，SPR仪器 一直是科罗拉多大学生物物理核心的重要组成部分 Anschutz医疗校园（CU-AMC）已被用来支持各种各样的NIH资助 项目。但是，经过二十年后，设备变得不可靠，维护成本高昂， 过时，很快就无法修复。我们建议购买新的SPR仪器 要替换我们当前的乐器，确保SPR继续作为我们校园的关键部分 研究基础设施。该仪器将被安置在一个既定，良好的管理中，并且 资助的生物物理学核心设施将允许维护并与专业知识相结合。 该工具将由多个基础科学和临床部门的研究人员使用 并将成为培训下一代生物物理学家和生物化学家的关键部分。 此外，与旧仪器相比，新仪器的增强功能将 允许使用材料少得多的实验，并具有更大的分子， 并以更高的灵敏度以更高的方式。 收购这项新工具将对NIH资助产生直接而持久的影响 在像传染病，病毒学，癌症，RNA剪接，细胞等潜水区的领域进行研究 蛋白质定位，基因调节，免疫学和药物设计。这些好处（以及 其他）研究领域包括时间和物质节省，更快的数据收集，更高的灵敏度， 能够在更多潜水条件下收集数据，更好的小分子结合检测和 增强的培训机会。