Surface Plasmon Resonance Instrumentation

项目摘要 实验技术，使定量询问之间的相互作用 不同的生物分子对生物医学研究至关重要。这些技术提供了 重要的数据，以了解不同疾病的分子基础，开发新的机制， 模型，发现新的药物和疗法，并指导其他实验。表面等离子体 共振（SPR）已经成为一种值得信赖的，强大的，多功能的方法来探索 分子结合事件的热力学和动力学。二十年来，SPR仪器 一直是科罗拉多大学生物物理学核心的重要组成部分 安舒茨医学院（CU-AMC），并已被用于支持各种NIH资助的 项目然而，二十年后，设备变得不可靠，维护成本高， 过时了，很快就无法修复了。我们建议购买新的SPR仪器 以取代我们目前的仪器，确保SPR继续作为我们校园的一个重要组成部分。 研究基础设施。该仪器将被安置在一个既定的，管理良好的， 资助的生物物理学核心设施，将使其得以维护，并与专业知识相结合。 这台仪器将被来自多个基础科学和临床部门的研究人员使用 并将成为培养下一代生物药理学家和生物化学家的关键部分。 此外，与旧仪器相比，新仪器的增强功能将 允许用更少的材料进行实验，用更多种类的分子， 并且以具有更高灵敏度的更高通量的方式。 购买这一新仪器将对NIH资助的 研究领域广泛，如传染病、病毒学、癌症、RNA剪接、细胞 蛋白定位、基因调控、免疫学和药物设计。这些好处（和 其他）研究领域包括节省时间和材料，更快的数据收集，更高的灵敏度， 在更多样化的条件下收集数据的能力，更好的小分子结合检测， 增加培训机会。

PROJECT SUMMARY Experimental techniques that enable quantitative interrogation of the interactions between different biological molecules are critical to biomedical research. Such techniques provide important data to understand the molecular basis of diverse diseases, develop new mechanistic models, discover new drugs and therapies, and guide other experiments. Surface plasmon resonance (SPR) has emerged as a trusted, robust, and versatile method to explore the thermodynamics and kinetics of molecular binding events. For twenty years, SPR instrumentation has been an important and integral part of the Biophysics Core on the University of Colorado Anschutz Medical Campus (CU-AMC) and has been used to support a wide variety of NIH-funded projects. However, after two decades the equipment has become unreliable, costly to maintain, obsolete, and will soon no longer be repairable. We propose to purchase a new SPR instrument to replace our current instrument, ensuring SPR continues as a critical part of our campus’ research infrastructure. The instrument will be housed in an established, well-managed, and funded Biophysics Core facility that will allow it to be maintained and to be coupled with expertise. This instrument will be used by researchers from multiple basic science and clinical departments and will be a key part of training the next generation of biophysicists and biochemists. Furthermore, compared to the old instrument, the new instrument’s enhanced capabilities will allow for experiments to be conducted with much less material, with a greater variety of molecules, and in a higher-throughput way with greater sensitivity. Acquisition of this new instrument will have an immediate and lasting impact on NIH-funded research in areas as diverse as infectious diseases, virology, cancer, RNA splicing, cellular protein localization, gene regulation, immunology, and drug design. The benefits to these (and other) research areas include time and material savings, faster data collection, greater sensitivity, ability to collect data under more diverse conditions, better small molecule binding detection, and enhanced training opportunities.