NCCAT: National Center for CryoEM Access and Training

NCCAT：国家冷冻电镜访问和培训中心

• 批准号: 10394723
• 负责人: Jeffrey S Kieft
• 金额: $ 500万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10394723
• 关键词: Adopted; Area; Cryoelectron Microscopy; Custom; Data; Data Collection; Derivation procedure; Educational Status; Educational workshop; Electron Microscopy; Ensure; Environment; Equipment; Generations; Goals; Human Resources; Immersion; Infrastructure; Intention; Laboratory Research; Macromolecular Complexes; Maps; Measures; Methods; Microscope; Modeling; New York; Phase; Positioning Attribute; Preparation; Process; Production; Quality Control; Records; Research Personnel; Resolution; Resources; Robot; Running; Sampling; Science; Services; Site; Specimen; Structure; System; Time; Titan; Training; Training Activity; Training Programs; Training and Infrastructure; base; biological systems; career; computerized data processing; data acquisition; density; detector; expectation; image processing; innovation; instrumentation; operation; particle; reconstruction; screening; skills; structural biology; tomography

Summary We propose to establish the National Center for CryoEM Access and Training (NCCAT) to be housed at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCCAT will provide access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team will provide direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. An already well established cross-training program will be expanded and extended to provide training across a wide variety of skill levels and career goals. NCCAT will adopt an open and transparent application process to ensure equal-opportunity nationwide access. The operations at NCCAT will build on already established practices at SEMC/NYSBC and benefit from the overall vibrant environment as well as the expertise of many of the highly trained staff already in place. NCCAT will be housed in new, custom-built space that will provide optimal, environmentally stable conditions. The major instrumentation will consist of 3 Titan Krios TEMs equipped with direct detectors, energy filters and phase plates, and 3 screening microscopes equipped with CMOS cameras. Streamlined workflows, already well established, will expanded to ensure that we collect the highest quality data as efficiently as possible. A user service operation will provide on-site, remote-access, and mail-in data collection services with fair and equal access to a nationwide user base. We will guide and assist users with all aspects of cryoEM, including sample preparation, data collection and image processing. A training program will encompass a full range of expectations, from the basics required to ensure that users produce valid structures, through more detailed training for users seeking independence, to fully embedded researchers who will be expected to graduate to running their own cryoEM research laboratories. While we anticipate that we will initially be called to serve and train mostly in the area of single particle cryoEM, we are well positioned to provide support in the area of cryoET that we predict will grow rapidly. While the goals of NCCAT are not innately innovative it is our intention to set the standard as one of the first world class, nationally accessible cryoEM access and training facilities that is freely and fairly available to all researchers in the US, and to continually strive to enable science of the highest quality and value.

概括 我们建议建立国家冷冻电镜访问和培训中心 (NCCAT)，设在 西蒙斯电子显微镜中心 (SEMC)，隶属于纽约结构生物学中心 （NYSBC），总部位于纽约州纽约市。 NCCAT 将提供最先进的设备，包括 标本制备机器人、高端显微镜和直接检测器、筛选显微镜以及所有 使用低温电子以尽可能高的分辨率解析结构所需的其他辅助设备 显微镜（冷冻电镜）方法。高素质的技术团队将提供直接支持、指导和 协助确保在最短的时间内生产出最高质量的结构。一个 已经完善的交叉培训计划将得到扩展和延伸，以提供跨领域的培训 各种各样的技能水平和职业目标。 NCCAT将采用公开透明的申请流程 确保全国范围内的机会均等。 NCCAT 的运营将建立在 SEMC/NYSBC 已建立的实践基础上，并受益于 整体充满活力的环境以及许多训练有素的员工的专业知识已经就位。 NCCAT 将坐落在新的定制空间中，该空间将提供最佳的、环境稳定的条件。这 主要仪器将包括 3 个 Titan Krios TEM，配备直接探测器、能量过滤器和 相位板和 3 个配备 CMOS 相机的筛选显微镜。已经简化了工作流程 完善后，将进行扩展，以确保我们尽可能高效地收集最高质量的数据。一个 用户服务运营将公平、公正地提供现场、远程访问、邮寄数据采集服务。 平等地获得全国用户群。我们将在冷冻电镜的各个方面为用户提供指导和帮助，包括 样品制备、数据收集和图像处理。培训计划将涵盖全方位 期望，从确保用户生成有效结构所需的基础知识，到更详细的 为寻求独立的用户提供培训，为预计毕业的完全嵌入的研究人员提供培训 经营自己的冷冻电镜研究实验室。虽然我们预计我们最初会被召唤去服务和 主要在单粒子冷冻电镜领域进行培训，我们有能力在以下领域提供支持 我们预测cryoET将快速增长。 虽然 NCCAT 的目标并非天生具有创新性，但我们的目的是将该标准设定为首批标准之一 世界一流、全国范围内均可使用的冷冻电镜访问和培训设施，免费且公平地向所有人开放 美国的研究人员，并不断努力实现最高质量和价值的科学。