NCCAT: National Center for CryoEM Access and Training--Screening Supplement

NCCAT：国家冷冻电镜访问和培训中心 - 筛选补充

• 批准号: 10830733
• 负责人: Jeffrey S Kieft
• 金额: $ 27.93万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10830733
• 关键词: Cryoelectron Microscopy; Dedications; Ensure; Equipment; Funding; Goals; Image; Macromolecular Complexes; Methods; Microscope; Modeling; New York; Preparation; Procedures; Production; Qualifying; Resolution; Resources; Robot; Sampling; Services; Specimen; Standardization; Structure; Support Groups; Training; Training Programs; Transmission Electron Microscopy; biological systems; career; detector; experience; particle; screening; screening services; skills; structural biology; supplemental screening

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art cryo-electron microscopy (cryoEM) equipment. The equipment and services include specimen preparation robots, high-end microscopes with direct detectors, screening microscopes, and other ancillary equipment required to solve structures to the highest possible resolution using cryoEM methods. A highly qualified technical team provides direct support, guidance, and assistance, ensuring the highest quality structures are resolved quickly and efficiently. A cross-training program offers training to users across the full spectrum of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo-transmission electron microscopy (cryoTEM) at NCCAT. The objective is to provide urgently needed support to groups with limited experience in cryoEM methods and limited access to local facilities. The goals will be to support these groups and get them started, while also training them in all aspects of the methods so they can make the best use of every resource available to them as soon as possible.

项目概要/摘要 国家冷冻电镜访问和培训中心 (NCCAT)，位于纽约结构研究所 生物中心提供最先进的冷冻电子显微镜 (cryoEM) 设备。这 设备和服务包括标本制备机器人、具有直接功能的高端显微镜 探测器、筛选显微镜和其他解决结构问题所需的辅助设备 使用冷冻电镜方法获得尽可能高的分辨率。高素质的技术团队提供直接 支持、指导和协助，确保快速解决最高质量的结构 高效。交叉培训计划为用户提供全方位技能水平和 职业目标。 这是一项补充资金请求，以支持网格准备和筛选 (GPS) 服务 用于 NCCAT 的冷冻透射电子显微镜 (cryoTEM)。目标是紧急提供 需要对冷冻电镜方法经验有限且当地接触机会有限的团体提供支持 设施。目标将是支持这些团体并让他们开始，同时对他们进行所有方面的培训 方法的各个方面，以便他们能够尽快充分利用可用的所有资源 可能的。

项目成果

Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryoelectron microscopy.

• DOI: 10.1073/pnas.2302531120
• 发表时间: 2023-06-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Watkins, Maxwell B.;Wang, Haoyue;Burnim, Audrey;Ando, Nozomi
• 通讯作者: Ando, Nozomi

Absolute structure determination of Berkecoumarin by X-ray and electron diffraction.

通过 X 射线和电子衍射测定 Berkecoumarin 的绝对结构。

• DOI: 10.1107/s2053229624003061
• 发表时间: 2024
• 期刊: Acta crystallographica. Section C, Structural chemistry
• 作者: Decato,Daniel;Palatinus,Lukáš;Stierle,Andrea;Stierle,Donald
• 通讯作者: Stierle,Donald

Structure of the human cation-chloride cotransport KCC1 in an outward-open state.

• DOI: 10.1073/pnas.2109083119
• 发表时间: 2022-07-05
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1

Structural diversity of the SARS-CoV-2 Omicron spike.

• DOI: 10.1016/j.molcel.2022.03.028
• 发表时间: 2022-06-02
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Gobeil SM;Henderson R;Stalls V;Janowska K;Huang X;May A;Speakman M;Beaudoin E;Manne K;Li D;Parks R;Barr M;Deyton M;Martin M;Mansouri K;Edwards RJ;Eaton A;Montefiori DC;Sempowski GD;Saunders KO;Wiehe K;Williams W;Korber B;Haynes BF;Acharya P
• 通讯作者: Acharya P

Structural polymorphism of amyloid fibrils in ATTR amyloidosis revealed by cryo-electron microscopy.

• DOI: 10.1038/s41467-024-44820-3
• 发表时间: 2024-01-17
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Nguyen BA;Singh V;Afrin S;Yakubovska A;Wang L;Ahmed Y;Pedretti R;Fernandez-Ramirez MDC;Singh P;Pękała M;Cabrera Hernandez LO;Kumar S;Lemoff A;Gonzalez-Prieto R;Sawaya MR;Eisenberg DS;Benson MD;Saelices L
• 通讯作者: Saelices L