NCCAT: National Center for CryoEM Access and Training--Screening Supplement

项目摘要/摘要 国家冷冻EM访问和培训中心(NCCAT)，总部设在纽约结构 生物中心，提供最先进的冷冻电子显微镜(CryoEM)设备。这个 设备和服务包括样品准备机器人、高端显微镜和直接 探测器、筛选显微镜和其他辅助设备，以解决结构到 使用低温电磁方法的最高分辨率。一支高素质的技术团队可为您提供 支持、指导和协助，确保快速解决最高质量的结构 效率很高。交叉培训计划为用户提供全方位的技能水平和 职业目标。 这是为支持格网准备和筛选(GPS)服务而申请的补充资金 用于NCCAT的低温电子显微镜(CryoTEM)。目标是紧急提供 需要为在冷冻EM方法方面经验有限且访问当地渠道有限的团体提供支持 设施。我们的目标将是支持这些群体并让他们开始工作，同时对他们进行全面的培训 方法的方方面面，以便他们可以最大限度地利用他们可用的每一种资源 有可能。

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art cryo-electron microscopy (cryoEM) equipment. The equipment and services include specimen preparation robots, high-end microscopes with direct detectors, screening microscopes, and other ancillary equipment required to solve structures to the highest possible resolution using cryoEM methods. A highly qualified technical team provides direct support, guidance, and assistance, ensuring the highest quality structures are resolved quickly and efficiently. A cross-training program offers training to users across the full spectrum of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo-transmission electron microscopy (cryoTEM) at NCCAT. The objective is to provide urgently needed support to groups with limited experience in cryoEM methods and limited access to local facilities. The goals will be to support these groups and get them started, while also training them in all aspects of the methods so they can make the best use of every resource available to them as soon as possible.