Animal and Biostatistics Core

动物和生物统计学核心

• 批准号: 10816719
• 负责人: TODD E MORGAN
• 金额: $ 24.05万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-15 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10816719
• 关键词: Age; Animal Experiments; Animals; Aorta; Archives; Autopsy; Behavioral; Biological; Biostatistics Core; Blood; Body Composition; Body Temperature; Body Weight; Bone Marrow; Brain; Breeding; C-Peptide; Cardiac; Cell Aging; Cells; Cellular Stress; Cognitive; Collection; Complement; Cysteine; Dedications; Diet; Dose; Ensure; Environment; FRAP1 gene; Fasting; Freezing; Gases; Genetic; Genotype; Glucose; Goals; Growth; Heart; Hematopoietic; Hematopoietic System; Hematopoietic stem cells; Hippocampus; Histopathology; Hypothalamic structure; IGFBP1 gene; Immune; Incidence; Individual; Insulin; Insulin-Like Growth Factor I; Intervention; Kidney; Knock-out; Link; Liver; Longevity; Lyase; Mediator; Metabolic; Microscopic; Mitochondria; Molecular; Molecular Profiling; Monitor; Mus; Natural regeneration; Neoplasms; Nervous System; Noise; Organ; Outcome; Pancreas; Pathology; Pathway interactions; Peptides; Physical activity; Pilot Projects; Procedures; Production; Proteins; Protocols documentation; Records; Regulation; Rejuvenation; Reperfusion Injury; Research Personnel; Resistance; Sampling; Security; Skeletal Muscle; Specific qualifier value; Standardization; Stress; Testing; Tissue Banks; Tissues; Toxic effect; Transgenic Organisms; Work; age related; biological adaptation to stress; chemotherapy; cohort; cytokine; design; dietary restriction; experimental study; feeding; healthspan; histopathological examination; humanin; in vivo; liver ischemia; mimetics; mortality; mouse model; pharmacologic; programs; protective effect; tissue regeneration

Abstract The Animal and Biostatistics Core will work directly with Project Leaders to design, plan, monitor and interpret all animal experiments, as well as, provide specific mouse models and standardized experimental protocols in support of the overall program's common goals: 1) understand how age and genetic background modulate the effects of fasting-mimicking diets (FMD) and protein restriction cycles (PRC) on longevity and healthspan, 2) study the mechanisms of FMD-, PRC-dependent cellular protection, regeneration and rejuvenation in the hematopoietic and nervous systems, 3) understand the link between FMD and PRC, GH-IGF-1, mTOR and humanin to test the hypothesis that this mitochondrial peptide is a PFC/PRC mediator with the potential to serve as a fasting/protein restriction mimetic, 4) study the regulation of the cysteine gamma lyase-dependent production of the gas H2S, negatively regulated by GH/IGF-1-mTOR, and test the hypothesis that H2S is a mediator of the protective effects of FMD on resistance to hepatic ischemia reperfusion injury and chemotherapy-induced toxicity to hematopoietic stem cells. The core provides an integrative framework for assessment of common outcomes across the individual projects needs. Aim 1. Design, plan and monitor animal experimental procedures as directed by Project Leaders. Studies are designed to achieve sufficient power/sensitivity to meet the stated objectives. All experimental procedures performed on mice are standardized and performed by Core B to ensure consistent assessment across all projects. These include: A) Longevity, B) Healthspan (Metabolic/Cognitive and Cellular/Molecular), C) Stress resistance and Mechanistic studies, D) Tissue collection/distribution. Aim 2. Coordinate animal use for program. All animals obtained or generated through Core B will be housed in the state-of-the-art vivarium facility in the Ray R Irani Hall at USC. Individual dedicated animal holding rooms for the exclusive use of either breeding, longevity or stress resistance will ensure consistency of biological environment, noise control and security across all studies.

抽象的 动物和生物统计学核心将直接与项目负责人一起设计，计划，监视和解释 所有动物实验以及在 支持整个计划的共同目标：1）了解年龄和遗传背景如何调节 禁食饮食（FMD）和蛋白质限制周期（PRC）对寿命和健康状态的影响，2） 研究FMD-依赖于PRC的细胞保护，再生和恢复活力的机制 造血和神经系统，3）了解FMD与PRC，GH-IGF-1，MTOR和MTOR和 人素检验了这种线粒体肽是PFC/PRC介质的假设，有可能 用作禁食/蛋白质限制模拟物，4）研究半胱氨酸γ酶依赖性的调节 气体H2S的产生，受GH/IGF-1-MTOR负调节，并测试H2S是一个假设 FMD对肝缺血再灌注损伤抗性的保护作用的介体 化学疗法引起的对造血干细胞的毒性。核心为 评估各个项目需求的共同结果。 目标1。按照项目负责人指示的设计，计划和监视动物实验程序。研究是 旨在实现足够的功率/灵敏度来实现既定目标。所有实验程序 对小鼠进行的进行标准化并由核心B进行，以确保所有人的一致评估 项目。其中包括：a）寿命，b）健康状态（代谢/认知和细胞/分子），c）应力 电阻和机械研究，d）组织收集/分布。 目标2。协调动物用于程序。通过核心B获得或生成的所有动物都将安置在 在南加州大学雷伊拉尼大厅的最先进的Vivarium设施。个人专用的动物握住房间 为了独家使用育种，寿命或耐压力，将确保生物学的一致性 所有研究的环境，噪声控制和安全性。