100 Additional fresh samples for verification of assay to replace the original proposed banked samples that cannot be used due to repeated exposure to freezing and thawing.

劳拉？詹姆斯 项目总结/摘要 开发AcetaSTAT的最初计划是使用收集的血清库样本 对乙酰氨基酚过量患者的样本，以在验证前验证检测的有效性 和验证工作。然而，在这项工作的发展过程中， 库存样品不能使用，因为它们不稳定，反复暴露于冷冻和再冷冻。 样品解冻。该测定旨在由临床医生立即使用，因此尽管它 似乎对样品老化敏感，这不会影响最终预期用途。但 由于缺乏最新的样品，项目推迟了，我们认为有必要 从接触过醋氨酚的病人身上采集新鲜样本

James, Laura P Project Summary/Abstract The original plan for development of AcetaSTAT was to use banked samples of serum collected from acetaminophen overdose patients to verify the effectiveness of the assay prior to verification and validation efforts. However, during the development of the work it has become apparent that banked samples cannot be used due to their instability and repeated exposure to freezing and re- thawing of samples. The assay is intended for immediate use by the clinician, so although it appears to be sensitive to sample age, this will not impact the final intended use. However, the lack of more recent samples has delayed the project and we have determined it will be necessary to collect fresh samples from patients with acetaminophen exposure.