100 Additional fresh samples for verification of assay to replace the original proposed banked samples that cannot be used due to repeated exposure to freezing and thawing.

詹姆士，劳拉·P 项目摘要/摘要 开发AcetaSTAT的最初计划是使用收集的血清样本 从对乙酰氨基酚过量患者那里验证该检测的有效性，然后再进行验证 和验证的努力。然而，在工作的发展过程中，显而易见的是 储存的样品不能使用，因为它们不稳定，而且反复暴露在冷冻和再... 样品解冻。该分析旨在供临床医生立即使用，因此尽管它 似乎对样品年龄很敏感，但这不会影响最终的预期用途。然而， 由于缺乏较新的样品，该项目被推迟，我们认为这是必要的。 采集对乙酰氨基酚暴露患者的新鲜样本。

James, Laura P Project Summary/Abstract The original plan for development of AcetaSTAT was to use banked samples of serum collected from acetaminophen overdose patients to verify the effectiveness of the assay prior to verification and validation efforts. However, during the development of the work it has become apparent that banked samples cannot be used due to their instability and repeated exposure to freezing and re- thawing of samples. The assay is intended for immediate use by the clinician, so although it appears to be sensitive to sample age, this will not impact the final intended use. However, the lack of more recent samples has delayed the project and we have determined it will be necessary to collect fresh samples from patients with acetaminophen exposure.