100 Additional fresh samples for verification of assay to replace the original proposed banked samples that cannot be used due to repeated exposure to freezing and thawing.

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James, Laura P Project Summary/Abstract The original plan for development of AcetaSTAT was to use banked samples of serum collected from acetaminophen overdose patients to verify the effectiveness of the assay prior to verification and validation efforts. However, during the development of the work it has become apparent that banked samples cannot be used due to their instability and repeated exposure to freezing and re- thawing of samples. The assay is intended for immediate use by the clinician, so although it appears to be sensitive to sample age, this will not impact the final intended use. However, the lack of more recent samples has delayed the project and we have determined it will be necessary to collect fresh samples from patients with acetaminophen exposure.