GlyCORE: Glycoscience Center of Research Excellence

GlyCORE：糖科学卓越研究中心

• 批准号: 10853237
• 负责人: SAMIR A ROSS
• 金额: $ 47.49万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10853237
• 关键词: Address; Adipocytes; Adopted; Affect; Amino Acids; Analytical Chemistry; Binding; Biological; Biological Assay; Biological Process; Biology; Biomedical Research; Blood capillaries; Bone Development; Carbohydrates; Cell Culture Techniques; Cell Survival; Cell physiology; Cells; Cellular biology; Centers of Research Excellence; Characteristics; Chemicals; Chronic; Collaborations; Collagen; Collagen Receptors; Confocal Microscopy; Core Facility; Data; Endothelial Cells; Energy Metabolism; Enzyme-Linked Immunosorbent Assay; Evaluation; Faculty; Fibroblasts; Fluorine; Foundations; Funding; Genus Hippocampus; Glycolysis; Glycoproteins; Goals; Growth; Health; Histopathology; Homeostasis; Human; Image; Inflammation; Injury; Label; Laboratories; Length; Link; Maine; Malignant - descriptor; Malignant Neoplasms; Mass Spectrum Analysis; Mediator; Mentors; Metabolic; Metabolism; Mississippi; Mitochondria; Modification; N-terminal; NMR Spectroscopy; National Institute of General Medical Sciences; Nature; Osteogenesis; Pharmaceutical Chemistry; Play; Post-Translational Protein Processing; Process; Protein Secretion; Proteins; Recombinants; Regenerative Medicine; Regulation; Research; Research Institute; Research Personnel; Research Project Grants; Resources; Respiration; Role; Science; Site; Structure; Structure-Activity Relationship; System; Techniques; Therapeutic Uses; Tissues; Umbilicus; Universities; Variant; Work; biophysical chemistry; career; clinical translation; experience; glycosylated collagen; glycosylation; healing; interdisciplinary approach; lipid biosynthesis; meetings; metabolic abnormality assessment; protein expression; recruit; stem; stem cells; synergism; tissue injury; tissue repair; tool; translational potential; triple helix; wound healing

Project Summary Understanding tissue healing and protein expression after injury is crucial in regenerative medicine. The glycoprotein CTHRC1 plays a vital role in tissue repair, regulation of fat cell formation, and bone development. It is produced by activated fibroblasts and contributes to metabolic efficiency and the survival of endothelial cells, which maintain homeostasis and capillary networks during healing. However, abnormal CTHRC1 expression is observed in chronic inflammation as well as malignant growth. While glycosylation is essential for the proper functioning of many secreted proteins, we don't yet understand how glycosylation impacts the beneficial effects of CTHRC1. CTHRC1 is a secreted protein with a 16-amino acid pro-peptide at its N-terminus. Removing this pro-peptide increases the biological activity of the protein. Our recent findings indicate that both the full-length and truncated forms of CTHRC1 promote glycolysis in endothelial cells, with the truncated form being particularly potent. Even though these initial structure–activity observations highlight the importance of protein modifications in controlling activity of CTHRC1, no further studies exploring the relationship between structure and function of CTHRC1 have been conducted. Additionally, the specific receptor for CTHRC1 remains unknown. In this collaborative project, our goal is to investigate how glycosylation of CTHRC1 influences its binding to target cells and its role in regulating energy metabolism. We will also track the cellular localization of internalized CTHRC1 by chemically tagging different amino acid residues to elucidate its site of action. Understanding the pro-glycolytic effects of CTHRC1 is instrumental in comprehending the functioning of endothelial cells during ongoing tissue repair. This proposal aims to develop CTHRC1 into a valuable tool to better understand the characteristics of activated endothelial cells during wound healing. To accomplish this objective, we will adopt an interdisciplinary team-science approach that builds upon the expertise and resources of the Centers for Biomedical Research Excellence (COBRE) supported by NIGMS in Mississippi and Maine. By leveraging state-of-the-art glycoscience techniques, we aim to address the challenges faced in the field of regenerative medicine.

项目总结

项目成果

Red Algal Sulfated Galactan Binds and Protects Neural Cells from HIV-1 gp120 and Tat.

• DOI: 10.3390/ph14080714
• 发表时间: 2021-07-23
• 期刊: Pharmaceuticals (Basel, Switzerland)
• 作者: Pomin VH;Mahdi F;Jin W;Zhang F;Linhardt RJ;Paris JJ
• 通讯作者: Paris JJ

Inhibition of SARS-CoV-2 wild-type (Wuhan-Hu-1) and Delta (B.1.617.2) strains by marine sulfated glycans.

• DOI: 10.1093/glycob/cwac042
• 发表时间: 2022-09-19
• 期刊: Glycobiology
• 影响因子: 4.3

Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery.

• DOI: 10.3390/microorganisms9071376
• 发表时间: 2021-06-24
• 期刊: Microorganisms
• 影响因子: 4.5
• 作者: Ahearne A;Albataineh H;Dowd SE;Stevens DC
• 通讯作者: Stevens DC

Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator.

• DOI: 10.1038/s41598-021-96046-8
• 发表时间: 2021-08-12
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Akbar S;Stevens DC
• 通讯作者: Stevens DC

De Novo Design of a Self-Assembled Artificial Copper Peptide that Activates and Reduces Peroxide.

• DOI: 10.1021/acscatal.1c02132
• 发表时间: 2021-08-20
• 期刊: ACS CATALYSIS
• 影响因子: 12.9
• 作者: Mitra, Suchitra;Prakash, Divyansh;Rajabimoghadam, Khashayar;Wawrzak, Zdzislaw;Prasad, Pallavi;Wu, Tong;Misra, Sandeep K.;Sharp, Joshua S.;Garcia-Bosch, Isaac;Chakraborty, Saumen
• 通讯作者: Chakraborty, Saumen