POPULATION GENETICS OF TSETSE FLIES

采采蝇的群体遗传学

• 批准号: 2650041
• 负责人: ELLIOT Scoville KRAFSUR
• 金额: $ 22.18万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-03-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2650041
• 关键词: Africa; Diptera; SDS polyacrylamide gel electrophoresis; animal population genetics; arthropod borne communicable disease; communicable disease control; disease vectors; field study; gene frequency; gene interaction; genetic markers; genetic polymorphism; genotype; insect control; mitochondrial DNA; nucleic acid sequence; polymerase chain reaction; single strand conformation polymorphism; statistics /biometry; trypanosomiasis

Tsetse flies (Glossina spp) constitute and important and widespread block to economic development in much of sub-Sahara Africa because they are the vectors of African sleeping sickness of humans and nagano of cattle. Virtually nothing is known of their population genetics. Although all tsetse have low reproduction rates (probably the least of all insects), the populations are highly resilient and control measures have proven ineffective except against geographically marginal populations. Genetically based methods of tsetse control have long been advocated and work is proceeding on several fronts. Will cultured, release tsetse disperse and mate with their feral cousins? Are there genetically differentiated natural populations of tsetse among which gene flow is greatly reduced or altogether lacking? Are there cryptic species? We propose to measure the extent of genetic variation at the DNA level in the Morsitans species group of tsetse and partition spatially that estimates effective dispersal rates at micro to macro geographical scales. Thus we would achieve estimates of gene flow within and among tsetse populations and sub-populations. The project would provide indices of reproductive isolation, gene flow, and genetic differentiation among conspecific tsetse populations in the southern African and East African fly belts. Genetic markers based on nuclear DNA sequences (microsatellite loci), maternally inherited markers in mtDNA, and allozyme loci will be used to provide the genotypes of individual flies in demes. The data would allow inferences to be drawn about the susceptibility of tsetse to methods of control that depend on matings between cultured and released forms with flies in natural population, e.g., sterile males and transgencic files.

采采蝇（舌蝇属）是构成采采蝇重要而广泛分布的种群

项目成果

Microsatellite diversities and gene flow in the tsetse fly, Glossina morsitans s.l.

采采蝇 (Glossina morsitans s.l.) 的微卫星多样性和基因流

• DOI: 10.1046/j.1365-2915.2002.00378.x
• 发表时间: 2002
• 期刊: Medical and veterinary entomology
• 影响因子: 1.9
• 作者: Krafsur,ES;Endsley,MA
• 通讯作者: Endsley,MA

Breeding structure of Glossina pallidipes populations evaluated by mitochondrial variation.

• DOI: 10.1093/jhered/90.6.635
• 发表时间: 1999-11
• 期刊: The Journal of heredity
• 作者: E. Krafsur;DL Wohlford

Patterns of genetic diversity and differentiation in the tsetse fly Glossina morsitans morsitans Westwood populations in East and southern Africa.

东非和南部非洲采采蝇 Glossina morsitans morsitans Westwood 种群的遗传多样性和分化模式。

• DOI: 10.1007/s10709-006-9001-0
• 发表时间: 2007
• 期刊: Genetica
• 影响因子: 1.5
• 作者: Ouma,JO;Marquez,JG;Krafsur,ES
• 通讯作者: Krafsur,ES

Population structure of the tsetse fly Glossina pallidipes estimated by allozyme, microsatellite and mitochondrial gene diversities.

通过等位酶、微卫星和线粒体基因多样性估计采采蝇 Glossina pallidipes 的种群结构。

• DOI: 10.1046/j.0962-1075.2001.00307.x
• 发表时间: 2002
• 期刊: Insect molecular biology
• 影响因子: 2.6
• 作者: Krafsur,ES

Genetic differentiation of some Glossina morsitans morsitans populations.

一些 Glossina morsitans morsitans 种群的遗传分化。

• DOI: 10.1046/j.1365-2915.1999.00185.x
• 发表时间: 1999
• 期刊: Medical and veterinary entomology
• 影响因子: 1.9
• 作者: Wohlford,DL;Krafsur,ES;Griffiths,NT;Marquez,JG;Baker,MD
• 通讯作者: Baker,MD