PROLACTIN AND PROLACTIN RECEPTORS IN T CELL DEVELOPMENT

T 细胞发育中的催乳素和催乳素受体

• 批准号: 2017235
• 负责人: HOLLY M. BROWN-BORG
• 金额: $ 10.19万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2017235
• 关键词: CD antigens; T lymphocyte; biomarker; cell differentiation; developmental immunology; embryo /fetus; flow cytometry; hematopoietic stem cells; hormone receptor; laboratory mouse; liver cells; newborn animals; nucleotidyltransferase; phenotype; polymerase chain reaction; prolactin; southern blotting

DESCRIPTION: Previous studies have suggested that prolactin (PRL) may help to regulate T cell development. These observations include showing that PRL and PRL-R mRNA are expressed by thymus, T cells and thymic epithelial cells, and the lack of PRL seems to increase the percentage of immature thymocytes. There also exists a body of correlative or suggestive evidence, such as showing that PRL-R expression coincides with the time that hematopoietic stem cells migrate from the fetal liver to primary lymphoid organs. Finally, the Ames dwarf mouse, which is deficient in PRL, exhibits a variety of immunodeficiencies that can be reversed by PRL therapy. This proposal seeks to determine what role, if any, that PRL plays during T cell development. First, expression of PRL-R by cells from fetal liver and thymocytes recovered at various stages of development will be evaluated. The maturation of T cells in Ames dwarf mice will be studied by following expression of T cell surface maturation markers. Finally, thymocytes exposed to PRL in vitro will be examined for terminal deoxynucleotidyl transferase expression by RT-PCR.

先前的研究表明，催乳素（PRL）可能有帮助