Studying the interaction of Flor-Essence Tonic and PHIP

研究 Flor-Essence Tonic 与 PHIP 的相互作用

• 批准号: 6727426
• 负责人: KRISTEN S KULP
• 金额: $ 28.02万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6727426
• 关键词: DNA damage; adduct; alternative medicine; breast neoplasms; cancer risk; cell line; laboratory rat; medicinal plants; mutagens; neoplastic growth; nutrition related neoplasm /cancer

DESCRIPTION (provided by applicant): Flor-Essence(R) Herbal Tonic is a widely used and self-administrated complementary and alternative medicine often consumed by breast cancer patients to supplement conventional therapy. The eight herbs used in Flor-Essence(R) have importance in the Asian diet and medicines and have estrogenic, cytotoxic, anti-estrogenic, antimutagenic, and anti-oxidant characteristics which are associated with altering cancer risk in animals and humans. PhlP is a ubiquitous dietary mutagen. It is carcinogenic in rats and has been linked to increased cancer risk in humans. Our preliminary data indicate Flor-Essence(R) can inhibit the mutagenicity and cytotoxicity of PhIP. It is critical that commercially available dietary supplements be studied in model systems relevant to current human consumption and exposure. The hypothesis of this proposal is that Flor-Essence(R) has properties that interfere with PhlP-induced mammary cancer risk in humans. The Comet assay and the Sprague Dawley rat model will be used to test this hypothesis. Aim 1: To determine if Flor-Essence(R) alters the induction or repair of PhlP-induced DNA adducts in human cancer cell lines. Single strand DNA breaks formed after PhlP exposure can be quantified using the alkaline comet assay. Breast derived cell lines that differ in metabolizing enzyme activity and tumorigenic potential will be used to quantify PhlP-induced damage. Flor-Essence(R) will be administered to the cells before, during, or after PhlP exposure in order to determine if the timing of herbal tonic exposure affects the induction and repair of PhlP induced damage. Aim 2: To determine if PhlP induced mammary tumor development in the Sprague Dawley rat model is altered by exposure to Flor-Essence(R) Herbal tonic. PhlP will be used to induce mammary tumors in 7 week old rats consuming (a) water or 3% Flor-Essence(R) (b) throughout the study, (c) prior to and during carcinogen exposure, (d) after PhlP. Mammary tumor incidence, multiplicity, latency, and tumor pathologies will be compared. These experiments are designed to determine (a) if Flor-Essence(R) can inhibit the mutagenicity of PhlP in mammalian cells and (b) if Flor-Essence(R) exposure influences PhlP-induced mammary tumor development in rats. It is important to determine how currently used CAMs are interacting with dietary carcinogens to influence health. Science based information is needed so that people can make informed lifestyle choices.

描述（由申请人提供）： Flor-Essence(R) Herbal Tonic 是一种广泛使用的自我管理的补充和替代药物，经常被乳腺癌患者用来补充常规治疗。 Flor-Essence(R) 中使用的八种草药在亚洲饮食和药物中具有重要意义，并具有雌激素、细胞毒性、抗雌激素、抗突变和抗氧化特性，这些特性与改变动物和人类的癌症风险有关。 PhlP 是一种普遍存在的膳食诱变剂。它对大鼠具有致癌性，并与人类癌症风险增加有关。我们的初步数据表明Flor-Essence(R) 可以抑制PhIP 的致突变性和细胞毒性。至关重要的是，在与当前人类消费和接触相关的模型系统中研究市售膳食补充剂。 该提案的假设是 Flor-Essence(R) 具有干扰 PhlP 诱发的人类乳腺癌风险的特性。彗星试验和斯普拉格道利大鼠模型将用于检验这一假设。 目标 1：确定 Flor-Essence(R) 是否会改变人类癌细胞系中 PhlP 诱导的 DNA 加合物的诱导或修复。 PhlP 暴露后形成的单链 DNA 断裂可以使用碱性彗星测定进行定量。代谢酶活性和致瘤潜力不同的乳腺细胞系将用于量化 PhlP 诱导的损伤。 Flor-Essence(R) 将在暴露于 PhlP 之前、期间或之后施用于细胞，以确定暴露草药滋补的时间是否会影响 PhlP 引起的损伤的诱导和修复。 目标 2：确定在 Sprague Dawley 大鼠模型中暴露于 Flor-Essence(R) 草本补品是否会改变 PhlP 诱导的乳腺肿瘤发展。 PhlP 将用于在 7 周大的大鼠中诱导乳腺肿瘤，这些大鼠在整个研究过程中消耗 (a) 水或 3% Flor-Essence(R) (b)、(c) 在接触致癌物之前和期间、(d) 在 PhlP 之后。将比较乳腺肿瘤的发病率、多样性、潜伏期和肿瘤病理学。这些实验旨在确定 (a) Flor-Essence(R) 是否可以抑制哺乳动物细胞中 PhlP 的致突变性，以及 (b) Flor-Essence(R) 暴露是否会影响 PhlP 诱导的大鼠乳腺肿瘤的发展。确定当前使用的 CAM 如何与膳食致癌物相互作用以影响健康非常重要。需要基于科学的信息，以便人们能够做出明智的生活方式选择。