BIOGENIC AMINE SYSTEMS, CRF, AND STRESS

描述（申请人摘要）：这是一份针对高级科学家的提案 由研究科学家资助的研究者颁发的奖项（K 05） 发展奖（K 02）过去10年。之前的K 02资金推动了 PI的职业生涯提供时间来开发新的方法和培养生产力 合作。在研究层面上，这导致了从单一到 3个主要的相互关联的NIH资助的研究项目。在技术层面上， 专业知识从主要的体内电生理方法， 包括体内微透析，以及复杂的系统和细胞 神经解剖学技术在职业一级，PI设立了一个 哈内曼精神病学系的应激神经生物学 大学]。2月，该司迁往儿童医院， 在费城，PI被提供了一个着名的职位， 宾夕法尼亚大学的研究员和教员职位。的 拟议的研究计划整合了3个资助研究项目的各个方面， 解决全球假说，即促肾上腺皮质激素释放因子的影响， 对生物胺系统的影响是急性应激反应的组成部分， 这些相互作用的应力诱导塑性是某些 压力相关的精神病理学拟议的职业发展计划将使 PI开发并将新的细胞方法纳入实验 目前用于阐明CRF-生物胺的系统方法库 应力诱导塑性的相互作用和机制。这些包括 离体脑片细胞分子电生理技术 制备和使用病毒载体来操纵CRF在 特异性CRF传入生物胺核。这些技术将武装 PI与一系列必要的工具，以解决研究问题的多个 （从系统到系统）。职业发展的第二个方面是 动员CHOP/ Upenn社区内的基础和临床研究人员， 在压力神经生物学中心对压力有着共同的兴趣。 电子通信、定期研究研讨会、务虚会和分享 公共资源用于促进 教师。因为该中心将把重点放在压力作为一个病因， 而不是针对特定的疾病，它有望成为一种理想的机制， 了解和开发新的治疗方法， 紊乱与之前的K 02奖一样，K 05奖将促进未来的 通过提供稳定的薪酬支持和灵活性， 加强技术技能和概念基础，促进领导能力。

DESCRIPTION (applicant's abstract): This is a proposal for a Senior Scientist Award (K05) from an investigator who has been funded by a Research Scientist Development Award (K02) for the last 10 years. Prior K02 funding advanced the PI's career by providing time to develop new approaches and foster productive collaborations. At a research level this resulted in expansion from a single to 3 major interrelated NIH-funded research programs. At a technical level, expertise went from predominantly in vivo electrophysiological approaches to including in vivo microdialysis, as well as sophisticated systems and cellular neuroanatomical techniques. At a career level, the PI established a Division of Stress Neurobiology within the Department of Psychiatry [at Hahnemann University]. In February the Division moved to the Children's Hospital of Philadelphia where the PI was offered a prestigious position as a Stokes Investigator and faculty position at the University of Pennsylvania. The proposed research plan integrates aspects of the 3 funded research programs to address the global hypothesis that the impact of corticotropin-releasing factor on biogenic amine systems is integral in the acute stress response and that stress-induced plasticity of these interactions underlies certain stress-related psychopathology. The proposed career development plan will allow the PI to develop and incorporate new cellular approaches into the experimental repertoire of systems approaches currently used to elucidate CRF-biogenic amine interactions and mechanisms underlying stress-induced plasticity. These include cellular and molecular electrophysiological techniques in in vitro slice preparations and the use of viral vectors to manipulate CRF expression in specific CRF afferents to biogenic amine nuclei. These techniques will arm the PI with a range of tools necessary to address research questions on multiple levels (from cellular to systems). A second aspect of career development is to mobilize basic and clinical investigators within the CHOP/ Upenn community who share a common interest in stress in a Center for Stress Neurobiology. Electronic communications, regular research seminars, retreats and shared common resources are used to foster interactions and collaborations among faculty. Because the Center will focus on stress as an etiological factor, rather than on a specific disease, it promises to be an ideal mechanism for understanding and developing new treatments for diverse medical and behavioral disorders. Like the previous K02 award, the K05 award will promote future career development by providing stable salary support and flexibility to enhance technical skills and conceptual bases and promoting leadership.