BIOGENIC AMINE SYSTEMS, CRF, AND STRESS

生物胺系统、CRF 和压力

• 批准号: 6228975
• 负责人: RITA VALENTINO
• 金额: $ 12.38万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6228975
• 关键词: antidepressants; autoradiography; biological signal transduction; corticotropin releasing factor; dorsal raphe nucleus; hormone receptor; immunocytochemistry; laboratory rat; locus coeruleus; neural plasticity; neural transmission; neurotransmitters; psychopharmacology; stress

DESCRIPTION (applicant's abstract): This is a proposal for a Senior Scientist Award (K05) from an investigator who has been funded by a Research Scientist Development Award (K02) for the last 10 years. Prior K02 funding advanced the PI's career by providing time to develop new approaches and foster productive collaborations. At a research level this resulted in expansion from a single to 3 major interrelated NIH-funded research programs. At a technical level, expertise went from predominantly in vivo electrophysiological approaches to including in vivo microdialysis, as well as sophisticated systems and cellular neuroanatomical techniques. At a career level, the PI established a Division of Stress Neurobiology within the Department of Psychiatry [at Hahnemann University]. In February the Division moved to the Children's Hospital of Philadelphia where the PI was offered a prestigious position as a Stokes Investigator and faculty position at the University of Pennsylvania. The proposed research plan integrates aspects of the 3 funded research programs to address the global hypothesis that the impact of corticotropin-releasing factor on biogenic amine systems is integral in the acute stress response and that stress-induced plasticity of these interactions underlies certain stress-related psychopathology. The proposed career development plan will allow the PI to develop and incorporate new cellular approaches into the experimental repertoire of systems approaches currently used to elucidate CRF-biogenic amine interactions and mechanisms underlying stress-induced plasticity. These include cellular and molecular electrophysiological techniques in in vitro slice preparations and the use of viral vectors to manipulate CRF expression in specific CRF afferents to biogenic amine nuclei. These techniques will arm the PI with a range of tools necessary to address research questions on multiple levels (from cellular to systems). A second aspect of career development is to mobilize basic and clinical investigators within the CHOP/ Upenn community who share a common interest in stress in a Center for Stress Neurobiology. Electronic communications, regular research seminars, retreats and shared common resources are used to foster interactions and collaborations among faculty. Because the Center will focus on stress as an etiological factor, rather than on a specific disease, it promises to be an ideal mechanism for understanding and developing new treatments for diverse medical and behavioral disorders. Like the previous K02 award, the K05 award will promote future career development by providing stable salary support and flexibility to enhance technical skills and conceptual bases and promoting leadership.

描述（申请人的摘要）：这是针对高级科学家的提案 由研究科学家资助的研究者颁发的奖项 (K05) 近10年发展奖（K02）。之前的 K02 资金推动了 PI 的职业生涯通过提供时间来开发新方法并培养生产力 合作。在研究层面上，这导致了从单一扩展到 NIH 资助的 3 个主要相互关联的研究项目。从技术层面来说， 专业知识从主要是体内电生理方法到 包括体内微透析，以及复杂的系统和细胞 神经解剖技术。在职业层面，PI 设立了一个部门 精神病学系的压力神经生物学 [哈尼曼 (Hahnemann) 大学]。 2月，科室迁至儿童医院。 费城，PI 获得了斯托克斯 (Stokes) 的享有盛誉的职位 宾夕法尼亚大学研究员和教职。这 拟议的研究计划整合了 3 个资助研究项目的各个方面 解决促肾上腺皮质激素释放因子的影响这一总体假设 生物胺系统是急性应激反应中不可或缺的一部分 这些相互作用的应力诱导可塑性是某些 压力相关的精神病理学。拟议的职业发展计划将允许 PI 开发新的细胞方法并将其纳入实验 目前用于阐明 CRF 生物胺的系统方法库 应力诱导可塑性的相互作用和机制。这些包括 体外切片细胞和分子电生理技术 制剂和使用病毒载体来操纵CRF表达 特定的 CRF 传入生物胺核。这些技术将武装 PI 拥有解决多个研究问题所需的一系列工具 级别（从细胞到系统）。职业发展的第二个方面是 动员 CHOP/Upenn 社区内的基础和临床研究人员 在压力神经生物学中心对压力有着共同的兴趣。 电子通讯、定期研究研讨会、静修和分享 共同资源用于促进相互之间的互动和合作 学院。因为该中心将重点关注压力作为病因因素， 它不是针对特定疾病，而是有望成为一种理想的机制 了解并开发针对不同医学和行为的新疗法 失调。与之前的K02奖一样，K05奖将推动未来 通过提供稳定的薪资支持和灵活性来实现职业发展 增强技术技能和概念基础并提升领导力。