TOPIC 403: DEVELOPMENT OF SPATIAL MICROPURIFICATION TECHNOLOGY

肿瘤微环境（TME）包括细胞类型的混合物，包括肿瘤细胞、癌症亚克隆、化生、发育异常、结缔组织增生、反应性成纤维细胞和复杂的免疫应答。测量TME内每个细胞组分的分子状态对于理解肿瘤的发生和进展以及确定癌症和正常宿主细胞之间的相互作用是重要的。 该提案描述了一种用于组织学载玻片的新的DNA测序和基因组分析技术，称为空间微纯化，该技术可以开发成一种商业设备，允许对TME进行局部的细胞特异性询问。SMP设备可以分析单个靶细胞，称为单细胞空间微纯化（Single-Cell SMP），或者可以获得相同谱系的靶细胞组，例如肿瘤细胞或淋巴细胞，称为多细胞空间微纯化（Multi-Cell SMP）。总之，SMP的两个版本为癌症肿瘤微环境（TME）的基因组分析提供了一个灵活的平台，研究人员可以使用该平台来满足他们的研究和临床需求。 这种用于肿瘤空间分子谱分析的新技术的开发和部署将提供对细胞基因型和形态学之间关系的更深入理解， 在癌症患者中开发新的诊断、预后或治疗干预措施。

The tumor microenvironment (TME) comprises a mixture of cell types, including tumor cells, cancer sub-clones, metaplasia, dysplasia, desmoplasia, reactive fibroblasts, and a complex immune response. Measuring the molecular status of each cellular component within the TME is important to understanding tumor initiation and progression as well as in determining the interplay between cancer and normal host cells. The proposal describes a new DNA sequencing and genomic analysis technology for histological slides called spatial micropurification, which can be developed into a commercial device that permits localized, cell-specific interrogation of the TME. SMP devices can analyze individual target cells, called single cell spatial micropurification (Single-Cell SMP), or can procure groups of target cells of the same lineage, such as tumor cells or lymphocytes, called multiple cell spatial micropurification (Multi-Cell SMP). Together, the two versions of SMP provide a flexible platform for genomic analyses of the cancer tumor microenvironment (TME) that investigators can employ to meet their research and clinical needs. Development and deployment of this novel technology for spatial molecular profiling of tumors will provide a deeper understanding of the relationship between cell genotype and morphology, providing valuable information toward development of new diagnostic, prognostic, or therapeutic interventions in patients with cancer.