Ultra-high speed AO-OCT clinical system to image ganglion cells and microglia

超高速 AO-OCT 临床系统对神经节细胞和小胶质细胞进行成像

• 批准号: 10547181
• 负责人: Mircea Mujat
• 金额: $ 87.56万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10547181
• 关键词: Area; Biological Markers; Blindness; Buffers; Cells; Clinical; Collaborations; Communities; Data; Degenerative Disorder; Detection; Development; Diabetes Mellitus; Diabetic Retinopathy; Diagnosis; Disease; Disease Marker; Disease Progression; Ear; Elements; Evaluation; Eye; Ganglia; Glaucoma; Goals; Government; Human; Image; Imaging Techniques; Investigation; Label; Lateral; Lead; Legal patent; Market Research; Massachusetts; Microglia; Monitor; Morphologic artifacts; Motion; Nerve Fibers; Neurosciences; Noise; Ophthalmology; Ophthalmoscopes; Optics; Pathogenesis; Patients; Performance; Persons; Phagocytosis; Pharmacotherapy; Phase; Play; Recording of previous events; Research; Resolution; Retina; Retinal Degeneration; Retinal Diseases; Retinal Ganglion Cells; Role; Scanning; Severity of illness; Signal Transduction; Source; Spatial Distribution; Speed; Structure; System; Technology; Testing; Time; Vision; adaptive optics; axon injury; base; clinical diagnostics; clinical imaging; cost; density; design; diagnostic platform; experience; ganglion cell; healthy volunteer; human subject; imager; imaging approach; imaging platform; imaging system; improved; improved functioning; innovation; instrument; interest; lens; macrophage; next generation; novel; novel therapeutics; optical imaging; physical science; programs; prototype; retinal imaging; routine imaging; specific biomarkers; tool

Project Summary/Abstract There is currently a significant need for new imaging techniques that may enable accurate quantification of biomarkers for detection, diagnosis, and monitoring of retinal diseases progression. Physical Sciences Inc. (PSI), Massachusetts Eye and Ear (MEE), and Joslin Diabetes Center (JDC) propose to develop a clinical tool able to routinely image retinal ganglion cells (RGCs) and to characterize microglia spatial distribution and temporal dynamics in live human eyes using a label-free adaptive optics (AO) imaging approach for improved diagnosis and treatment of glaucoma and diabetic retinopathy (DR). An ultra-high speed AO-OCT system will be designed and built based on a number of recently demonstrated innovations: lens-based design, ultra-high speed to eliminate motion artifacts, buffered swept source (SS) and dual beam approach to increase A-line rate to the MHz range, adaptive lens, dual-axis MEMS for raster scanning, and the use of polarization optics to eliminate specular reflections on optical elements. The retinal imager will be tested in a clinical setting at MEE and JDC on a large group of glaucoma and DR subjects experiencing a wide range of disease severity. Specific biomarkers for glaucoma and DR will be defined based on patient data acquired by our collaborators from MEE and JDC. PSI has a long, successful history of developing and commercializing high-resolution retinal imagers for the ophthalmic research market which gives us a competitive advantage in developing and maturing this platform that enables routine clinical imaging of human subjects and motivates a Direct to Phase II approach. A successful program and subsequent Phase III commercial development will provide clinicians with high performance retinal imaging for glaucoma and DR investigations at a lower cost and improved functionality superior to other non-AO retinal imagers. Early adaptors of this technology within the research community will grow our understanding of vision and its disruption by glaucoma and DR and will investigate the effects of new drugs and therapies. The ability to visualize ganglion and macrophage cells without fluorescent labeling in the human eye represents an important advance for both ophthalmology and neuroscience, which will lead to identification of novel disease biomarkers and new avenues of exploration in disease progression.

项目总结/摘要 目前存在对新成像技术的显著需求，所述新成像技术可以实现准确的成像。 用于检测、诊断和监测视网膜疾病进展的生物标志物的定量。 物理科学公司(PSI)、马萨诸塞州眼耳中心和乔斯林糖尿病中心 我建议开发一种能够常规成像视网膜神经节细胞（RGC）并表征 使用无标记自适应光学在活体人眼中小胶质细胞的空间分布和时间动态 (AO)影像学方法，用于改善青光眼和糖尿病视网膜病变（DR）的诊断和治疗。 本论文将基于近年来的一些研究成果，设计并搭建超高速声光OCT系统， 展示创新：基于镜头的设计，超高速消除运动伪影，缓冲扫描 源（SS）和双光束方法，以增加A线速率到MHz范围，自适应透镜，双轴 MEMS用于光栅扫描，并使用偏振光学器件消除光学器件上的镜面反射。 元素视网膜成像仪将在临床环境中进行测试，在一个大的群体， 青光眼和DR受试者经历广泛的疾病严重程度。青光眼的特异性生物标志物 和DR将根据我们的合作者从CNAS和JDC获得的患者数据进行定义。 PSI在开发和商业化高分辨率视网膜成像仪方面有着悠久而成功的历史， 眼科研究市场，这使我们在开发和成熟这一领域具有竞争优势。 该平台能够对人类受试者进行常规临床成像，并促进直接进入II期 approach.一个成功的项目和随后的第三阶段商业开发将为临床医生提供 具有高性能的视网膜成像，用于青光眼和DR检查，成本更低， 功能上级于其他非AO视网膜成像器。这项技术在研究中的早期应用 社区将增加我们对视力的理解，以及青光眼和DR对视力的破坏，并将调查 新药物和疗法的效果。能够可视化神经节和巨噬细胞， 人眼中的荧光标记代表了眼科学和生物医学的重要进展。 神经科学，这将导致识别新的疾病生物标志物和新的探索途径 在疾病进展中。