DNA segregation during Bacillus growth and development

芽孢杆菌生长和发育过程中的 DNA 分离

• 批准号: 7492397
• 负责人: JOSEPH A POGLIANO
• 金额: $ 2.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7492397
• 关键词: Actins; Affect; Animal Model; Antibiotic Resistance; Antibiotics; Bacillus (bacterium); Bacteria; Bacterial DNA; Behavior; Biological; Biology; Cells; Cellular biology; DNA; DNA Sequence; Development; Diffusion; Distant; Ensure; Evolution; Family; Filament; Future; Generations; Genes; Genetic; Goals; Gram-Negative Bacteria; Growth; Growth and Development function; Homologous Gene; In Vitro; Indigenous; Individual; Infection; Inherited; Molecular Genetics; Mothers; Mutation; Numbers; Organism; Pathogenesis; Pathogenicity; Plasmid Cloning Vector; Plasmids; Play; Principal Investigator; Process; Proteins; Relative (related person); Replicon; Reproduction spores; Role; Site-Directed Mutagenesis; Study models; System; Testing; Toxin; Tubulin; Virulence; Virulence Factors; Work; capsule; in vivo; microbial; novel; plasmid DNA; programs; research study; segregation; size; tool; trait

The goal of this proposal is to identify plasmid DNA partitioning systemsthat are active during both . vegetative growth and sporulation in two different species of Bacillus. New tools for elucidating the mechanisms underlying plasmid segregation will be developed using the model organism B. subtilis, and then applied to B. megaterium. Utilizing two different Bacillus strains to study this problem will allow us to take advantage of the unique strengths that each organism and its indigenous plasmids offer, while maintaining a focused goal of understanding how conservedelements of the microbial DNA segregation machinery functions during sporulation to ensure that every spore inherits at least one copy of the plasmid. Plasmid segregation during sporulation provides an attractive system for studying DNA segregation because: 1) both the plasmids and the sporulation process are not nonessential; 2) the nearly 10 fold smaller volume of the forespore comparedto the mothercell demands an efficient plasmid partitioning system; 3) cell-specifictranscription factorsbecome active during sporulation, allowing the development of . powerful genetic and cell biology assaysfor DNA segregation during sporulation; and 4) many Bacillus plasmids do not encode proteins related to either the ParA or ParM families of plasmid segregation proteins, suggesting that novel partitioning mechanisms are at work. Since a wide variety of virulence factors are encoded on plasmids in pathogenic strains of Bacillus, understanding how plasmids are transmitted from one generation to the next is important for understanding the evolution and spread these key virulence determinants. These studies will allow the development of better genetic tools and plasmid vectors useful for manipulating many Bacillus species.

本提案的目标是鉴定在这两个过程中都有活性的质粒DNA分割系统。