MRI contrast agents targeting carbohydrate biomarkers

针对碳水化合物生物标志物的 MRI 造影剂

• 批准号: 7295681
• 负责人: Binghe Wang
• 金额: $ 19.11万
• 依托单位: GEORGIA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-26 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7295681
• 关键词: Affinity; Antibodies; Binding; Biological; Biological Markers; Boronic Acids; Cancer Patient; Cancerous; Carbohydrates; Carcinoma; Cell surface; Cells; Characteristics; Chemistry; Contrast Media; Dendrimers; Detection; Development; Digit structure; Evaluation; Feasibility Studies; Foundations; Goals; Image; Immune response; Implant; Label; Lead; Lectin; Magnetic Resonance Imaging; Malignant - descriptor; Malignant Epithelial Cell; Malignant Neoplasms; Methods; Molecular Profiling; Monitor; Names; Numbers; Pharmacologic Substance; Phase; Play; Positioning Attribute; Preparation; Property; Range; Role; Specificity; Survival Rate; System; Technology; Work; base; boronic acid; cancer imaging; carbohydrate structure; cell type; cytotoxicity; improved; in vivo; in vivo Model; interest; research study; small molecule; tumor

DESCRIPTION (provided by applicant): Malignant transformation is often associated with alteration of cell surface carbohydrates. The expression or over-expression of certain carbohydrates, such as sialyl Lewis X (sLex), sialyl Lewis a (sLea), Lewis X (Lex) and Lewis Y (Ley), has been correlated with the development of certain cancers. These cell surface carbohydrates can be used for cell-specific identification and targeting of carcinoma cells. Recently, we have developed boronic acid-based small molecule lectin mimics (named boronolectins) that can recognize certain carbohydrates with selectivity. The same or similar methods can be used for the preparation of lectin mimics for a wide variety of carbohydrates. The long-term goal of this project is the development of conjugates of boronolectin-MRI contrast agents as biomarker-directed cancer imaging agents. Specifically, such conjugates can be used for the delivery of MRI contrast agents based on cell-surface carbohydrate biomarkers. In the R21 phase of this application, we plan to study the feasibility of this approach by (1) synthesizing boronolectin-MRI contrast agent conjugates using a boronolectin which is known selectively bind to sialyl Lewis X, (2) studying their ability to bind to cells with the target carbohydrate biomarkers, and (3) examining their ability to image implanted tumors in both an ex vivo and in vivo models. If the R21 phase is successful, in the R33 phase we plan to expand our biological evaluation to include tumors implanted at different positions, and to search for other lectin mimics that can bind specifically for other important carbohydrate-based cancer biomarkers. In addition, we also plan to examine the cytotoxicity of the boronolectin-MRI contrast agent conjugates. These small molecule-based recognition/delivery systems may have the following advantages over antibody-based systems: (1) greater stability during storage and in vivo; (2) lower propensity to elicit undesirable immune responses, (3) easier conjugation chemistry, and (4) more desirable pharmaceutical properties.

描述（由申请人提供）：恶性转化通常与细胞表面碳水化合物的改变有关。某些碳水化合物的表达或过表达，如sialyl Lewis X （sLex）、sialyl Lewis a （sLea）、Lewis X （Lex）和Lewis Y (Ley)，与某些癌症的发生有关。这些细胞表面碳水化合物可用于细胞特异性鉴定和靶向癌细胞。最近，我们开发了基于硼酸的小分子凝集素模拟物（命名为boronolectin），它可以选择性地识别某些碳水化合物。相同或类似的方法可用于制备多种碳水化合物的凝集素模拟物。该项目的长期目标是开发硼集素- mri造影剂的缀合物，作为生物标志物导向的癌症显像剂。具体地说，这种缀合物可用于递送基于细胞表面碳水化合物生物标志物的MRI造影剂。在该应用的R21阶段，我们计划通过(1)使用已知与sialyl Lewis X选择性结合的硼集素合成硼集素- mri造影剂偶联物来研究该方法的可行性，(2)研究它们与目标碳水化合物生物标志物结合细胞的能力，以及(3)在离体和体内模型中检查它们对植入肿瘤成像的能力。如果R21阶段是成功的，在R33阶段，我们计划扩大我们的生物学评估，包括在不同位置植入的肿瘤，并寻找其他凝集素模拟物，可以特异性结合其他重要的基于碳水化合物的癌症生物标志物。此外，我们还计划检查硼集素- mri造影剂偶联物的细胞毒性。与基于抗体的系统相比，这些基于小分子的识别/传递系统可能具有以下优点：(1)在储存和体内具有更高的稳定性；(2)降低引发不良免疫反应的倾向，(3)更容易偶联化学，(4)更理想的药物特性。