System for guided imaging and characterization of neural circuits

神经回路引导成像和表征系统

• 批准号: 7537519
• 负责人: JACOB R GLASER
• 金额: $ 27.2万
• 依托单位: MICROBRIGHTFIELD, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-10 至 2009-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7537519
• 关键词: Abbreviations; Alexa fluor 546; Area; Arts; Brain; Cells; Characteristics; Class; Collection; Computer software; Data; Dendritic Spines; Development; Devices; Distal; Effectiveness; Electrodes; Environment; Equipment; Feedback; Foundations; Future; Image; Intellectual Property; Legal patent; Life; MFC; Marketing; Methods; Microscope; Microscopic; Monitor; Morphology; Movement; Neurons; Neurophysiology - biologic function; Neurosciences; Neurosciences Research; Optics; Phase; Positioning Attribute; Preparation; Public Health; Radial; Relative (related person); Research; Research Personnel; Scanning; Slice; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Specimen; Structure; Synapses; System; Technology; Time; Tissues; Tracer; Water; base; biocytin; computerized; dentate gyrus; design; fluorophore; innovation; interest; lens; lucifer yellow; micromanipulator; nervous system disorder; neural circuit; neurotoxicology; novel; prototype; reconstruction; research and development; research study; sensor; software development

DESCRIPTION (provided by applicant): We propose the Sirius workstation, an innovative microscope system combining the capabilities of automated neuron reconstruction using guided image collection, cell electrophysiological recording, and cell filling in live tissue. Sirius will be the first commercially available system to a) enable direct recording of single distal synaptic connections formed between different areas, b) facilitate repeated monitoring of potential alterations in neuronal morphology during or as a result of stimulation, and c) permit simultaneous recording of one or more neurons formed between different areas in brain slices identified based on neuronal structure. This system will allow significant advancements in integrated studies of neural function and structure in basic neuroscience as well as in neurotoxicology and pharmacological research and development, representing clear progress beyond the state-of-the-art. PUBLIC HEALTH RELEVANCE The proposed project will enable new important studies in neuroscience research which are not currently feasible. A system that combines the capabilities of guided automated neuron reconstruction, cell electrophysiological recording and cell filling in live tissue will allow researchers to more accurately identify cells within a neural circuit, even those located at a distance from the original cell. The overall effect of this project will permit the collection of highly relevant data in integrated studies of neural function and structure in basic neuroscience, neurotoxicology, pharmacological research, and related fields involving research in neurological disease.

描述（由申请人提供）：我们提出了 Sirius 工作站，这是一种创新的显微镜系统，结合了使用引导图像采集、细胞电生理记录和活组织中的细胞填充进行自动神经元重建的功能。 Sirius 将是第一个商用系统，能够：a）能够直接记录不同区域之间形成的单个远端突触连接，b）有助于重复监测刺激期间或刺激导致的神经元形态的潜在变化，以及 c）允许同时记录根据神经元结构识别的脑切片中不同区域之间形成的一个或多个神经元。该系统将使基础神经科学中的神经功能和结构的综合研究以及神经毒理学和药理学研究和开发取得重大进展，代表着超越最先进水平的明显进展。公共卫生相关性拟议的项目将使目前尚不可行的神经科学研究中新的重要研究成为可能。一个结合了引导自动神经元重建、细胞电生理记录和活组织细胞填充功能的系统将使研究人员能够更准确地识别神经回路内的细胞，甚至是那些距离原始细胞较远的细胞。该项目的总体效果将有助于在基础神经科学、神经毒理学、药理学研究以及涉及神经疾病研究的相关领域的神经功能和结构综合研究中收集高度相关的数据。