NEURONAL GLIA CULTURE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拟议的核心设施便于UCC调查人员使用培养的神经元、神经胶质细胞和器官型培养。研究人员不再需要自己开发培养脑细胞或器官培养的技术。这些培养物被用于膜片钳、钙成像和疾病的体外模型等。核心设施由一名协调员和一名经验丰富的技术员组成。这里提出的培养核心设施的模型是基于加州大学洛杉矶分校的类似设施，在那里，一名技术人员为研究人员提供神经胶质和神经元的准备。拟议预算包括技术员、顾问和协调员的工资。需要为启动材料和用品、新项目以及顺利过渡到服务费制度提供资金。这一核心设施的目标是。A)在培养和对我们的文化进行持续质量控制方面，与新的方法并驾齐驱。为此，我们需要一位顾问来让我们了解最新情况。B)提供一种更快、更便宜的替代方案，并阻止在UCC各地启动新的神经元培养实验室。通过实现我们的目标，我们促进了高质量的研究，并提高了空间和设备的有效利用。通过提供脑细胞培养的专业知识和所涉及的技术劳动，这个核心设施为研究人员留出了更多的时间进行科学努力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed core facility facilitates the UCC investigators the use of cultured neurons, glia and organotypic cultures. The investigators no longer need to develop by themselves the techniques to culture brain cells or organotypic cultures. The cultures are used, among others, for patch clamping, Ca2+ imaging and for in vitro models of diseases. The core facility consists of a coordinator and an experience technician. The model of culture core facility proposed here is based on a similar facility in UCLA where a single technician provides glial and neuronal preparations to investigators. The proposed budget is includes salaries of the technician, consultant and the coordinator. Funds are requested for starting material and supplies, for new projects and to allow for a smooth transition to a system of fee for service. The objectives of this core facility are. a) To be abreast of new methodology in culturing and doing a constant quality control of our cultures. For that purpose we need a consultant to keep us up to date. b) To provide a faster, less expensive alternative and to discourage the initiation of new neuronal culture laboratories throughout the UCC. By fulfilling our goals we promote high quality research and increase the efficient use of space and equipment. By providing the expertise in brain cell culture and technical labor involved this core facility leaves more time to the investigators for the scientific endeavor.