NEURONAL GLIA CULTURE FACILITY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 拟议的核心设施促进UCC研究人员使用培养的神经元，神经胶质细胞和器官型培养物。 研究人员不再需要自己开发培养脑细胞或器官型培养的技术。 这些培养物尤其用于膜片钳、Ca 2+成像和疾病的体外模型。 核心设施由一名协调员和一名经验丰富的技术人员组成。 这里提出的培养核心设施的模型是基于加州大学洛杉矶分校的一个类似的设施，其中一个单一的技术人员提供神经胶质和神经元的准备工作的调查。 概算包括技术员、顾问和协调员的薪金。要求提供资金，用于新项目的起始材料和用品，以便顺利过渡到服务收费制度。 这一核心设施的目标是。 a）掌握新的培养方法，并对我们的培养物进行持续的质量控制。为此，我们需要一名顾问，让我们了解最新情况。 B）提供一种更快、更便宜的替代方案，并阻止在整个UCC启动新的神经元培养实验室。 通过实现我们的目标，我们促进高质量的研究，提高空间和设备的有效利用。 通过提供脑细胞培养和技术劳动方面的专业知识，这个核心设施为研究人员的科学奋进留下了更多的时间。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed core facility facilitates the UCC investigators the use of cultured neurons, glia and organotypic cultures. The investigators no longer need to develop by themselves the techniques to culture brain cells or organotypic cultures. The cultures are used, among others, for patch clamping, Ca2+ imaging and for in vitro models of diseases. The core facility consists of a coordinator and an experience technician. The model of culture core facility proposed here is based on a similar facility in UCLA where a single technician provides glial and neuronal preparations to investigators. The proposed budget is includes salaries of the technician, consultant and the coordinator. Funds are requested for starting material and supplies, for new projects and to allow for a smooth transition to a system of fee for service. The objectives of this core facility are. a) To be abreast of new methodology in culturing and doing a constant quality control of our cultures. For that purpose we need a consultant to keep us up to date. b) To provide a faster, less expensive alternative and to discourage the initiation of new neuronal culture laboratories throughout the UCC. By fulfilling our goals we promote high quality research and increase the efficient use of space and equipment. By providing the expertise in brain cell culture and technical labor involved this core facility leaves more time to the investigators for the scientific endeavor.