NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 8357098
• 负责人: P. A. Ferchmin
• 金额: $ 6.14万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357098
• 关键词: Antibodies; Astrocytes; Biochemistry; Biomedical Research; Budgets; Cell Culture Techniques; Cerebral cortex; Core Facility; Crowding; Development; Equipment; Funding; Grant; Hippocampus (Brain); Human Resources; Individual; Laboratories; Laboratory culture; Learning; Left; Methods; Monitor; National Center for Research Resources; Neonatal; Neuroglia; Neurons; Principal Investigator; Procedures; Quality Control; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Serum; Slice; Source; Speed; Structure; Time; Training; Travel; United States National Institutes of Health; Unmarried person; Wages; base; brain cell; cost; experience; fetal; interest; member; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The "Neuronal and Glial Culture Facility" or NGF is a core facility structured to provide cultures of interest to neuroscientists. In some cases, the NGF provides training and starting supplies to investigators that prefer to learn the procedures and then continue doing the cultures themselves. In each case, the NGF facilitates and speeds up research. The NGF does not occupy physical space, it uses the equipment of the central cell culture facility of the RCMI and the NGF technician uses the equipment and space of a laboratory in the Department of Biochemistry. Therefore, the NGF is based on a space and equipment-sharing basis. This core facility consists of a coordinator (PAF) and a technician, Mrs. Brenda Cuadrado (BC), experienced in neuronal cell culture. Among other skills, she is able to prepare neuronal cultures from fetal cortex or hippocampus, astrocytes from neonatal cerebral cortex, organotypic hippocampal slices using the Stoppini method. BC was trained at the UCC (by PAF), by Dr. C. Ghiani from UCLA and BC traveled to UK, Lexington (USA), to learn organotypic slice culture in the laboratory of Dr. Littletone. During the last year, Mrs. Cuadrado has learned immunocytochemical methods under the guide of Dr. H. Yeh (consultant), the NGF coordinator (PAF) and Dr. H. Martins. The immunocytochemical methods are used to monitor the cultures provided by the NGF. By dedicating one technician to produce a significant part of the cultures done at the UCC alleviates in part the crowding in the culture room, the duplication of equipment and saves sera and reagents. As result of this facility, the investigators no longer need to develop "from scratch" the cultures. This core facility is based on a similar arrangement in the laboratory of Dr. de Vellis' laboratory (UCLA) where a single person is dedicated to provide cultures for all the members of this prestigious group. The proposed budget consists of supplies and personnel. Salaries of the technician (BC) the coordinator (PAF) and the consultant costs are included. Supplies include media, sera, antibodies for quality control and further development of cultures. The objective of this unit is not to exclude anyone from using the existing cell culture facility but by providing a better alternative discourage the initiation of individual neuronal culture laboratories throughout the UCC. By doing so, we will increase the efficient use of space and equipment. By providing the expertise in brain cell culture and the labor involved, this core facility will leave more time to the investigators for the scientific endeavor.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 “神经元和胶质细胞培养设施”或NGF是一个核心设施，旨在为神经科学家提供感兴趣的培养物。 在某些情况下，神经生长因子提供培训和启动用品的研究人员更喜欢学习的程序，然后继续做文化本身。 在每一种情况下，神经生长因子促进和加快研究。 神经生长因子不占用物理空间，它使用RCMI中央细胞培养设施的设备，神经生长因子技术人员使用生物化学系实验室的设备和空间。 因此，神经营养基金是建立在空间和设备共享的基础上的。 该核心机构由一名协调员（PAF）和一名技术员Brenda Cuadrado女士（BC）组成，她在神经元细胞培养方面经验丰富。 在其他技能中，她能够使用Stoppini方法从胎儿皮层或海马中制备神经元培养物，从新生儿大脑皮层制备星形胶质细胞，制备器官型海马切片。 BC在UCC接受培训（由PAF），由C博士。来自加州大学洛杉矶分校和不列颠哥伦比亚省的Ghiani前往英国，列克星敦（美国），在Littletone博士的实验室学习器官型切片培养。 在过去的一年里，Cuadrado女士在H.叶（顾问），神经生长因子协调员（PAF）和H。马丁斯 免疫细胞化学方法用于监测由NGF提供的培养物。 通过专门安排一名技术人员生产在UCC完成的大部分培养物，在一定程度上缓解了培养室的拥挤、设备的重复，并节省了血清和试剂。 由于这种设施，研究人员不再需要“从头开始”培养。 这个核心设施是基于一个类似的安排，在德韦利斯博士的实验室（加州大学洛杉矶分校）的实验室，一个人致力于提供文化的所有成员，这个著名的集团。 拟议预算包括用品和人事费。包括技术员（BC）、协调员（PAF）的薪金和顾问费用。 供应品包括培养基、血清、抗体，用于质量控制和培养物的进一步开发。 这个单位的目的不是要排除任何人使用现有的细胞培养设施，但通过提供一个更好的替代方案，阻止整个UCC的个别神经元培养实验室的启动。 通过这样做，我们将提高空间和设备的有效利用。 通过提供脑细胞培养的专业知识和所涉及的劳动力，这个核心设施将为研究人员留下更多的时间进行科学奋进。