NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 8357098
• 负责人: P. A. Ferchmin
• 金额: $ 6.14万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357098
• 关键词: Antibodies; Astrocytes; Biochemistry; Biomedical Research; Budgets; Cell Culture Techniques; Cerebral cortex; Core Facility; Crowding; Development; Equipment; Funding; Grant; Hippocampus (Brain); Human Resources; Individual; Laboratories; Laboratory culture; Learning; Left; Methods; Monitor; National Center for Research Resources; Neonatal; Neuroglia; Neurons; Principal Investigator; Procedures; Quality Control; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Serum; Slice; Source; Speed; Structure; Time; Training; Travel; United States National Institutes of Health; Unmarried person; Wages; base; brain cell; cost; experience; fetal; interest; member; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The "Neuronal and Glial Culture Facility" or NGF is a core facility structured to provide cultures of interest to neuroscientists. In some cases, the NGF provides training and starting supplies to investigators that prefer to learn the procedures and then continue doing the cultures themselves. In each case, the NGF facilitates and speeds up research. The NGF does not occupy physical space, it uses the equipment of the central cell culture facility of the RCMI and the NGF technician uses the equipment and space of a laboratory in the Department of Biochemistry. Therefore, the NGF is based on a space and equipment-sharing basis. This core facility consists of a coordinator (PAF) and a technician, Mrs. Brenda Cuadrado (BC), experienced in neuronal cell culture. Among other skills, she is able to prepare neuronal cultures from fetal cortex or hippocampus, astrocytes from neonatal cerebral cortex, organotypic hippocampal slices using the Stoppini method. BC was trained at the UCC (by PAF), by Dr. C. Ghiani from UCLA and BC traveled to UK, Lexington (USA), to learn organotypic slice culture in the laboratory of Dr. Littletone. During the last year, Mrs. Cuadrado has learned immunocytochemical methods under the guide of Dr. H. Yeh (consultant), the NGF coordinator (PAF) and Dr. H. Martins. The immunocytochemical methods are used to monitor the cultures provided by the NGF. By dedicating one technician to produce a significant part of the cultures done at the UCC alleviates in part the crowding in the culture room, the duplication of equipment and saves sera and reagents. As result of this facility, the investigators no longer need to develop "from scratch" the cultures. This core facility is based on a similar arrangement in the laboratory of Dr. de Vellis' laboratory (UCLA) where a single person is dedicated to provide cultures for all the members of this prestigious group. The proposed budget consists of supplies and personnel. Salaries of the technician (BC) the coordinator (PAF) and the consultant costs are included. Supplies include media, sera, antibodies for quality control and further development of cultures. The objective of this unit is not to exclude anyone from using the existing cell culture facility but by providing a better alternative discourage the initiation of individual neuronal culture laboratories throughout the UCC. By doing so, we will increase the efficient use of space and equipment. By providing the expertise in brain cell culture and the labor involved, this core facility will leave more time to the investigators for the scientific endeavor.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 “神经元和胶质细胞培养设施”（NGF）是一个核心设施，旨在为神经科学家提供感兴趣的培养物。 在某些情况下，NGF 向喜欢学习程序然后继续自己进行培养的研究人员提供培训和起始用品。 在每种情况下，NGF 都会促进并加速研究。 NGF不占用物理空间，它使用RCMI中央细胞培养设施的设备，NGF技术人员使用生物化学系实验室的设备和空间。 因此，NGF是建立在空间和设备共享的基础上的。 该核心设施由一名协调员 (PAF) 和一名在神经细胞培养方面经验丰富的 Brenda Cuadrado 女士 (BC) 组成。 除其他技能外，她还能够使用 Stoppini 方法制备胎儿皮质或海马的神经元培养物、新生儿大脑皮质的星形胶质细胞、器官型海马切片。 BC 在 UCC（由 PAF）接受了来自加州大学洛杉矶分校 (UCLA) 的 C. Ghiani 博士的培训，随后 BC 前往英国、列克星敦（美国），在 Littletone 博士的实验室学习器官切片培养。 去年，Cuadrado女士在H. Yeh博士（顾问）、NGF协调员（PAF）和H. Martins博士的指导下学习了免疫细胞化学方法。 免疫细胞化学方法用于监测 NGF 提供的培养物。 通过指定一名技术人员来生产 UCC 中完成的大部分培养物，可以部分缓解培养室的拥挤情况、设备的重复，并节省血清和试剂。 由于有了这个设施，研究人员不再需要“从头开始”开发培养物。 该核心设施基于 de Vellis 博士实验室（加州大学洛杉矶分校）的类似安排，其中一个人致力于为这个享有盛誉的团体的所有成员提供培养物。 拟议预算包括物资和人员。包括技术员 (BC)、协调员 (PAF) 的工资和顾问费用。 耗材包括用于质量控制和进一步培养的培养基、血清、抗体。 该部门的目标不是排除任何人使用现有的细胞培养设施，而是通过提供更好的替代方案来阻止在整个 UCC 中建立单独的神经元培养实验室。 通过这样做，我们将提高空间和设备的有效利用。 通过提供脑细胞培养方面的专业知识和相关劳动力，该核心设施将为研究人员留下更多的时间进行科学研究。