NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 8357098
• 负责人: P. A. Ferchmin
• 金额: $ 6.14万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357098
• 关键词: Antibodies; Astrocytes; Biochemistry; Biomedical Research; Budgets; Cell Culture Techniques; Cerebral cortex; Core Facility; Crowding; Development; Equipment; Funding; Grant; Hippocampus (Brain); Human Resources; Individual; Laboratories; Laboratory culture; Learning; Left; Methods; Monitor; National Center for Research Resources; Neonatal; Neuroglia; Neurons; Principal Investigator; Procedures; Quality Control; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Serum; Slice; Source; Speed; Structure; Time; Training; Travel; United States National Institutes of Health; Unmarried person; Wages; base; brain cell; cost; experience; fetal; interest; member; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The "Neuronal and Glial Culture Facility" or NGF is a core facility structured to provide cultures of interest to neuroscientists. In some cases, the NGF provides training and starting supplies to investigators that prefer to learn the procedures and then continue doing the cultures themselves. In each case, the NGF facilitates and speeds up research. The NGF does not occupy physical space, it uses the equipment of the central cell culture facility of the RCMI and the NGF technician uses the equipment and space of a laboratory in the Department of Biochemistry. Therefore, the NGF is based on a space and equipment-sharing basis. This core facility consists of a coordinator (PAF) and a technician, Mrs. Brenda Cuadrado (BC), experienced in neuronal cell culture. Among other skills, she is able to prepare neuronal cultures from fetal cortex or hippocampus, astrocytes from neonatal cerebral cortex, organotypic hippocampal slices using the Stoppini method. BC was trained at the UCC (by PAF), by Dr. C. Ghiani from UCLA and BC traveled to UK, Lexington (USA), to learn organotypic slice culture in the laboratory of Dr. Littletone. During the last year, Mrs. Cuadrado has learned immunocytochemical methods under the guide of Dr. H. Yeh (consultant), the NGF coordinator (PAF) and Dr. H. Martins. The immunocytochemical methods are used to monitor the cultures provided by the NGF. By dedicating one technician to produce a significant part of the cultures done at the UCC alleviates in part the crowding in the culture room, the duplication of equipment and saves sera and reagents. As result of this facility, the investigators no longer need to develop "from scratch" the cultures. This core facility is based on a similar arrangement in the laboratory of Dr. de Vellis' laboratory (UCLA) where a single person is dedicated to provide cultures for all the members of this prestigious group. The proposed budget consists of supplies and personnel. Salaries of the technician (BC) the coordinator (PAF) and the consultant costs are included. Supplies include media, sera, antibodies for quality control and further development of cultures. The objective of this unit is not to exclude anyone from using the existing cell culture facility but by providing a better alternative discourage the initiation of individual neuronal culture laboratories throughout the UCC. By doing so, we will increase the efficient use of space and equipment. By providing the expertise in brain cell culture and the labor involved, this core facility will leave more time to the investigators for the scientific endeavor.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 “神经元和神经胶质细胞培养设施”或NGF是一个核心设施，旨在提供神经科学家感兴趣的培养。在某些情况下，NGF为那些更愿意学习程序然后继续自己进行培养的调查人员提供培训和启动用品。在每一种情况下，NGF都促进和加快了研究。NGF不占用物理空间，它使用RCMI中央细胞培养设施的设备，而NGF技术人员使用生化系实验室的设备和空间。因此，NGF是建立在空间和设备共享的基础上的。这一核心设施由一名协调员(PAF)和一名在神经细胞培养方面经验丰富的技术人员Brenda Cuadrado夫人(BC)组成。在其他技能中，她能够使用Stoppini方法准备来自胎儿皮质或海马区的神经元培养，来自新生大脑皮层的星形胶质细胞，以及器官型的海马片。BC由加州大学洛杉矶分校的C.Ghiani博士在UCC(由PAF)培训，BC前往英国列克星敦(美国)，在Littletone博士的实验室学习器官型切片培养。在过去的一年里，Cuadrado夫人在H.Yeh博士(顾问)、NGF协调员(PAF)和H.Martins博士的指导下学习了免疫细胞化学方法。免疫细胞化学方法用于监测由NGF提供的培养物。通过指定一名技术人员来生产在UCC完成的大量培养，在一定程度上缓解了培养室的拥挤、重复设备，并节省了血清和试剂。由于有了这个设施，调查人员不再需要“从头开始”发展培养。这一核心设施基于德韦利斯博士实验室(UCLA)的类似安排，一个人致力于为这个享有盛誉的团队的所有成员提供培养。拟议预算包括用品和人员。包括技术员(BC)、协调员(PAF)的工资和咨询费。供应品包括介质、血清、抗体，用于质量控制和进一步发展培养。该单位的目的并不是排除任何人使用现有的细胞培养设施，而是通过提供一个更好的替代方案来阻止在UCC各地启动单独的神经元培养实验室。通过这样做，我们将提高空间和设备的有效利用。通过提供脑细胞培养的专业知识和涉及的劳动力，这个核心设施将为研究人员留下更多的时间进行科学努力。