NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 7561501
• 负责人: P. A. Ferchmin
• 金额: $ 6.27万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561501
• 关键词: Astrocytes; Budgets; Cell Line; Cerebral cortex; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Conflict (Psychology); Core Facility; Crowding; Cultured Cells; Drug Addiction; Equipment; Fee-for-Service Plans; Funding; Future; Grant; Growth; Hippocampus (Brain); In Vitro; Incubators; Institution; Kentucky; Laboratories; Laboratory culture; Learning; Left; Membrane; Methods; Modeling; Neurodegenerative Disorders; Neuroglia; Neurons; Plastics; Platelet Activating Factor; Preparation; Research; Research Personnel; Resources; Schedule; Services; Slice; Source; System; Techniques; Time; Training; Travel; United States National Institutes of Health; Universities; Unmarried person; Wages; base; brain cell; brain tissue; cost; doxorubicin/fluorouracil/melphalan protocol; experience; fetal; in vitro Model; inhibitor/antagonist; patch clamp; prevent; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the past three years there was a noticeable increase in the use of the cell culture facility with the consequential crowding and scheduling conflicts. Most of the users use nerve cells, either neurons, glia or cell lines that are models of neural cells. The multiplicity of users increases costs, crowding and expense. By dedicating one technician for most of the preparations will alleviate most of those problems. The proposed core facility will facilitate the UCC investigators the use of cultured neurons, glias and organotypic cultures. The investigators will no longer need to develop by themselves the techniques to culture brain cells or brain tissue (organotypic cultures). The cultures will be used, among others, for patch clamping after in vitro treatments with drugs of addiction or inhibitors and for in vitro models of neurodegenerative diseases. The core facility will consist of a coordinator (PAF) and a technician (Brenda Cuadrado) who already has three years of experience in neuronal cell culture. Among other skills she is able to prepare neuronal cultures from fetal cortex or hippocampus or astrocytes from cerebral cortex. She has experience in culturing organotypic hippocampal slices on translucent and permeable plastic membranes using the Stoppini method. Mrs. Cuadrado was trained in the UCC (by PAF), by Dr. C. Ghiani from UCLA and she traveled to Lexington, Kentucky University, to learn organotypic slices culture in the laboratory of Dr. Littletone. The growth and continuous up grading of our services will be facilitated thanks to our close collaboration with Dr. J. de Vellis, who is an expert in glia and neuron culture. Dr. J. de Vellis recommended as a direct consultant Dr. Steve Levison. The model of the proposed service unit is based on the arrangement of Dr. de Vellis? laboratory (UCLA) with a single person dedicated to provide glial and neuronal preparations to this prestigious group. The proposed budget is mainly the salaries of the technician (Mrs. Cuadrado), consultant and the coordinator. Funds are requested for starting material and supplies to allow for a smooth transition to a system of fee for service. New equipment is requested to modernize and replace more than10 years old incubators and so prevent problems in the near future. The objective of this unit is not to exclude anyone from using the existing cell culture facility but by providing a better alternative discourage the initiation of new neuronal culture laboratories throughout the UCC. By doing so we will increase the efficient use of space and equipment. By providing the expertise in brain cell culture and the labor involved this core facility will leave more time to the investigators for the scientific endeavor.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在过去的三年中，随着相应的拥挤和调度冲突的使用，使用细胞培养设施的使用显着增加。 大多数用户使用神经细胞的神经细胞，是神经细胞模型的神经细胞。 用户的多样性增加了成本，拥挤和费用。 通过将一名技术人员用于大多数准备工作将减轻大多数问题。 拟议的核心设施将促进UCC研究人员的使用，使用培养的神经元，灰色和器官型培养物。 研究人员将不再需要自己开发培养脑细胞或脑组织（器官培养物）的技术。 除其他外，这些培养物将在用成瘾或抑制剂药物进行体外治疗后以及神经退行性疾病的体外模型后使用。 核心设施将由协调员（PAF）和技术人员（Brenda Cuadrado）组成，他们已经在神经元细胞培养中拥有三年的经验。 在其他技能中，她能够从胎儿皮质或海马或星形胶质细胞中制备神经元培养物。 她有使用Stoppini方法在半透明和可渗透塑料膜上培养器官海马切片的经验。 Cuadrado夫人接受了UCC的培训，由UCLA的C. Ghiani博士在UCC培训，她前往肯塔基大学列克星敦，在Littletone博士的实验室学习了Organtypic Slices文化。 由于我们与神经胶质和神经元文化专家J. de Vellis博士的密切合作，我们的服务的增长和持续分级将有助于我们的服务。 J. de Vellis博士建议担任直接顾问史蒂夫·莱维森博士。 拟议的服务部门的模型基于De Vellis博士的安排？实验室（UCLA）有一个专门为这个享有声望的群体提供神经胶质和神经元制剂的人。 拟议的预算主要是技术人员（Cuadrado夫人），顾问和协调员的薪水。要求资金用于起始材料和耗材，以使其平稳过渡到服务费系统。 要求新设备现代化和更换10多年历史的孵化器，因此在不久的将来防止问题。 该单元的目的不是排除任何人使用现有的细胞培养设施，而是通过提供更好的替代性劝阻整个UCC中新的神经文化实验室的启动。 通过这样做，我们将提高空间和设备的有效使用。 通过提供脑细胞培养的专业知识和涉及的劳动，该核心设施将为研究人员留出更多的时间进行科学努力。