NEURONAL GLIA CULTURE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 “神经胶质细胞培养设施”（NGF）是一个核心设施，旨在：1）为神经科学家提供细胞或组织培养; 2）培训，建议和支持新的研究人员进入这一领域; 3）为选择学习程序并自己进行培养的研究人员提供培训。 NGF是一个虚拟设施，因为它不占用物理空间，而是使用现有的实验室和设备。 神经生长因子人员包括一名协调员，P. A博士。Ferchmin，一位研究技术员，Brenda Cuadrado夫人，和一位访问顾问。这些服务包括来自产前皮层或海马的原代神经元培养物;来自新生儿大脑皮层的星形胶质细胞;器官型海马切片，以及自去年以来的干细胞和神经球。 此外，免疫细胞化学方法用于监测提供给研究者的培养物的质量。 拟议预算包括工作人员薪金、顾问费和用品。 将根据用户对新程序的需求选择顾问。 神经生长因子极大地促进了这些高度专业化的制剂的使用，为研究人员留下了更多的时间进行实验工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The "Neuronal Glia Culture Facility" (NGF) is a core facility programmed to: 1) provide cell or tissue cultures to neuroscientists; 2) train, advice and support investigators new to this field; 3) provide training to researchers that choose to learn the procedures and do the cultures themselves. NGF is a virtual facility since it does not occupy physical space, it uses existing labs and equipment. NGF personnel consist of a coordinator, Dr. P.A. Ferchmin, a research technician, Mrs. Brenda Cuadrado, and a visiting consultant. The services include primary neuronal cultures from prenatal cortex or hippocampus; astrocytes from neonatal cerebral cortex; organotypic hippocampal slices and since last year, stem cells and neurospheres. In addition, immunocytochemical methods are used to monitor the quality of the cultures provided to the investigators. The proposed budget consists of personnel salaries, consultant fees and supplies. The consultant will be chosen according to the users needs for new procedures. NGF greatly facilitates the use of these highly specialized preparations leaving more time to the investigators for experimental work.