NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 7959234
• 负责人: P. A. Ferchmin
• 金额: $ 6.47万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959234
• 关键词: Astrocytes; Biomedical Research; Budgets; Cells; Cerebral cortex; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Equipment; Fees; Funding; Grant; Hippocampus (Brain); Human Resources; Institution; Learning; Left; Methods; Monitor; Neonatal; Neuroglia; Neurons; Preparation; Procedures; Research; Research Personnel; Resources; Services; Slice; Source; Stem cells; Time; Training; United States National Institutes of Health; Visit; Wages; Work; prenatal; programs; tissue culture; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The "Neuronal Glia Culture Facility" (NGF) is a core facility programmed to: 1) provide cell or tissue cultures to neuroscientists; 2) train, advice and support investigators new to this field; 3) provide training to researchers that choose to learn the procedures and do the cultures themselves. NGF is a virtual facility since it does not occupy physical space, it uses existing labs and equipment. NGF personnel consist of a coordinator, Dr. P.A. Ferchmin, a research technician, Mrs. Brenda Cuadrado, and a visiting consultant. The services include primary neuronal cultures from prenatal cortex or hippocampus; astrocytes from neonatal cerebral cortex; organotypic hippocampal slices and since last year, stem cells and neurospheres. In addition, immunocytochemical methods are used to monitor the quality of the cultures provided to the investigators. The proposed budget consists of personnel salaries, consultant fees and supplies. The consultant will be chosen according to the users needs for new procedures. NGF greatly facilitates the use of these highly specialized preparations leaving more time to the investigators for experimental work.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 “神经胶质培养设施”（NGF）是一个编程的核心设施：1）为神经科学家提供细胞或组织培养； 2）训练，建议和支持该领域的调查人员； 3）为选择学习程序并自己进行文化的研究人员提供培训。 NGF是一种虚拟设施，因为它不占据物理空间，因此使用现有的实验室和设备。 NGF人员由协调员P.A. Ferchmin，一名研究技术员，Brenda Cuadrado夫人，访问顾问。这些服务包括产前皮层或海马的原发性神经元培养物；来自新生儿大脑皮层的星形胶质细胞；自去年以来，有机型海马切片，干细胞和神经球。 此外，使用免疫细胞化学方法来监测提供给研究人员提供的培养物的质量。 拟议的预算包括人员薪水，顾问费和用品。 顾问将根据用户需要的新程序选择。 NGF极大地促进了这些高度专业化的准备工作的使用，这给研究人员提供了更多的时间进行实验性工作。