NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 7336003
• 负责人: P. A. Ferchmin
• 金额: $ 6万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336003
• 关键词: NEURONAL; GLIA; CULTURE; FACILITY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the past three years there was a noticeable increase in the use of the cell culture facility with the consequential crowding and scheduling conflicts. Most of the users use nerve cells, either neurons, glia or cell lines that are models of neural cells. The multiplicity of users increases costs, crowding and expense. By dedicating one technician for most of the preparations will alleviate most of those problems. The proposed core facility will facilitate the UCC investigators the use of cultured neurons, glias and organotypic cultures. The investigators will no longer need to develop by themselves the techniques to culture brain cells or brain tissue (organotypic cultures). The cultures will be used, among others, for patch clamping after in vitro treatments with drugs of addiction or inhibitors and for in vitro models of neurodegenerative diseases. The core facility will consist of a coordinator (PAF) and a technician (Brenda Cuadrado) who already has three years of experience in neuronal cell culture. Among other skills she is able to prepare neuronal cultures from fetal cortex or hippocampus or astrocytes from cerebral cortex. She has experience in culturing organotypic hippocampal slices on translucent and permeable plastic membranes using the Stoppini method. Mrs. Cuadrado was trained in the UCC (by PAF), by Dr. C. Ghiani from UCLA and she traveled to Lexington, Kentucky University, to learn organotypic slices culture in the laboratory of Dr. Littletone. The growth and continuous up grading of our services will be facilitated thanks to our close collaboration with Dr. J. de Vellis, who is an expert in glia and neuron culture. Dr. J. de Vellis recommended as a direct consultant Dr. Steve Levison. The model of the proposed service unit is based on the arrangement of Dr. de Vellis? laboratory (UCLA) with a single person dedicated to provide glial and neuronal preparations to this prestigious group. The proposed budget is mainly the salaries of the technician (Mrs. Cuadrado), consultant and the coordinator. Funds are requested for starting material and supplies to allow for a smooth transition to a system of fee for service. New equipment is requested to modernize and replace more than10 years old incubators and so prevent problems in the near future. The objective of this unit is not to exclude anyone from using the existing cell culture facility but by providing a better alternative discourage the initiation of new neuronal culture laboratories throughout the UCC. By doing so we will increase the efficient use of space and equipment. By providing the expertise in brain cell culture and the labor involved this core facility will leave more time to the investigators for the scientific endeavor.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在过去三年中，细胞培养设施的使用明显增加，随之而来的是拥挤和调度冲突。大多数使用者使用神经细胞，无论是神经元、胶质细胞还是作为神经细胞模型的细胞系。用户的多样性增加了成本、拥挤和费用。指派一名技术人员负责大部分准备工作，将会缓解大部分问题。拟议的核心设施将促进UCC研究人员使用培养的神经元，胶质细胞和器官型培养。研究人员将不再需要自己开发培养脑细胞或脑组织的技术（器官型培养）。除其他外，培养物将用于成瘾或抑制剂药物体外治疗后的贴片夹紧，以及神经退行性疾病的体外模型。核心设施将由一名协调员（PAF）和一名已经有三年神经细胞培养经验的技术人员（Brenda Cuadrado）组成。在其他技能中，她能够从胎儿皮质或海马体或大脑皮质的星形胶质细胞中制备神经元培养物。她有使用Stoppini方法在半透明和可渗透塑料膜上培养器官型海马切片的经验。Cuadrado女士在加州大学洛杉矶分校的C. Ghiani博士的UCC（由PAF）培训下，前往肯塔基州列克星敦大学，在Littletone博士的实验室学习器官型切片培养。我们与神经胶质和神经元培养专家J. de Vellis博士的密切合作将促进我们服务的发展和持续升级。J. de Vellis博士推荐Steve Levison博士为直接顾问。拟议服务单位的模式是基于德韦利斯？一个人致力于为这个著名的群体提供胶质和神经元的准备。拟议预算主要是技术员（夸德拉多夫人）、顾问和协调员的薪金。要求为起始材料和用品提供资金，以便顺利过渡到服务收费制度。需要新的设备来现代化和取代超过10年的旧孵化器，从而防止在不久的将来出现问题。这个单位的目的不是要排除任何人使用现有的细胞培养设施，而是通过提供一个更好的替代方案来阻止在UCC建立新的神经元培养实验室。通过这样做，我们将提高空间和设备的有效利用。通过提供脑细胞培养的专业知识和相关的劳动，这个核心设施将为研究人员留下更多的时间进行科学研究。