DEFINING THE HCV INFECTIOUS WINDOW PERIOD IN THE CHIMPANZEE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 丙型肝炎病毒（HCV）的感染窗口期，即暴露于病毒和首次检测到病毒RNA之间的时间，尚未明确定义。 通过建立窗口期，可以进一步降低和/或预防血液制品传播HCV的风险。 目的是定义和表征HCV在黑猩猩模型中的感染窗口期。 已经选择了两只黑猩猩，并确定了基线肝酶水平。 HCV临界阳性和阴性血浆样本组正在为拟定研究进行整合。 一只黑猩猩静脉输注50 ml血浆，血浆来自通过核酸检测（NAT）检测为HCV阴性的一组中的5名个体供体。 这些动物被感染，表明HCV的存在低于NAT的检测水平。在代表时间正在进行额外的病毒学和免疫学检测。 病毒学分析表明，该动物迅速清除了感染，没有血清转化。暂停第二只动物的计划接种，直至最终决定使用不同的血浆盘。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infectious window period for hepatitis C virus (HCV), the time between exposure to the virus and the first detection of viral RNA, is not well defined. By establishing the window period, the risk of transmission of HCV by blood products can be further reduced and or prevented. The objective is to define and characterize the infectious window period for HCV in the chimpanzee model. Two chimpanzees have been selected and baseline liver enzyme levels have been determined. HCV borderline positive and negative plasma panels are being put together for the proposed studies. One chimpanzee was IV infused with 50 ml of plasma from each of five individual donors from a panel negative for HCV by nucleic acid testing (NAT). The animals become infected indicating that HCV was present below the level of detection of NAT. Additional virological and immunological assays are being performed at the represent time. Virological assays indicated that the animal rapidly cleared the infection and did not seroconvert. The planned inoculation of the second animal is on hold until a final decision is made on using a different plasma panel.