TRANSGENIC EXPRESSION OF TAU AND APP IN MODELS OF ALZHEIMER'S DISEASE

TAU 和 APP 在阿尔茨海默病模型中的转基因表达

• 批准号: 7562655
• 负责人: LARY C WALKER
• 金额: $ 3.95万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562655
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein Precursor; Brain; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Funding; Genes; Grant; Green Fluorescent Proteins; Human; Human Pathology; Incubated; Institution; Modeling; Pathology; Primates; Proteins; Rat-1; Rattus; Research; Research Personnel; Resources; Rodent; Saimiri; Source; Tauopathies; Transgenes; Transgenic Organisms; United States National Institutes of Health; aged; nonhuman primate; protein expression; tau Proteins; tau expression; tau mutation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The focus of this project was interactions between tau protein and the amyloid precursor protein (APP) in producing Alzheimer's disease pathology in the rodent (rat) and nonhuman primate (Saimiri sciureus) brain, with particular emphasis on tau protein in primates. These proteins are known to be critical to the development of Alzheimer's disease, but the existing models do not fully mimic the uniquely human pathology of Alzheimer's. We confirmed the long-term expression of the protein GFP in rats ( 1 year) as well as the induction of tau abnormalities by the protein. Genes for human-type tau protein are currently incubating in Saimiri, and we expect to have the results by early summer. We began an analysis of tau protein abnormalities in aged nonhuman primates to assess the natural occurrence of this pathology and its similarities to human tauopathies. Significant progress was made in developing a construct that enables the simultaneous expression of two separate transgenes.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的重点是Tau蛋白与淀粉样蛋白前体蛋白（APP）之间的相互作用在产生啮齿动物（大鼠）和非人类灵长类动物（Saimiri Sciureus）大脑中产生阿尔茨海默氏病病理学时，特别强调了灵长类动物中的tau蛋白。这些蛋白质对于阿尔茨海默氏病的发展至关重要，但现有模型并不能完全模仿阿尔茨海默氏症的独特人类病理学。 我们证实了蛋白质GFP在大鼠（1年）以及蛋白质诱导tau异常的长期表达。人型tau蛋白的基因目前正在Saimiri中孵化，我们希望到初夏天能够取得结果。我们开始对年迈的非人类灵长类动物的tau蛋白异常分析，以评估这种病理的自然发生及其与人tauopathies的相似之处。在开发一个能够同时表达两个单独转基因的构造方面取得了重大进展。