Animal Models Scientific Facilities Core

AM SFC 的总体目标是在一个地点提供专业设施、设备和 专业知识在动物模型中进行 BSL3 和 Select Agent 病原体的研究，这将促进 开发针对人畜共患病的新疫苗、疗法和其他生物防御产品。更多 具体来说，AM SFC 的目标是建立和利用生物防御产品开发： 1. 使用病毒和细菌病原体进行气溶胶吸入研究的设施和专业知识，需要 高水平遏制 2. 涉及利用啮齿类动物以及节肢动物传播的研究的设施和专业知识 较大的家畜和野生动物 3、研究生、博士后、技术人员、教职人员的发展培训 并利用气溶胶和媒介传播进行动物模型研究 动物模型核心人员将为多个级别的研究人员提供服务。在一些 案例中，核心人员将实质性协助实验设计，并负责所有工作 动物实验的各个方面，包括病原体的输送、监测和采样（例如血液） 来自动物，并进行安乐死和尸检。这种服务方式最适用于 来自其他机构的调查员和涉及私营公司的项目。服务的另一端 频谱将根据需要提供援助，特别是在气溶胶和媒介传播方面 病原体。在该项目的第一年，我们将在小鼠体内建立气溶胶模型并对其进行表征 三种病原体：土拉弗朗西斯菌LVS株、曲棍球脑炎病毒和狂犬病病毒。 这三种病原体都是重要的 BT 病原体的模型，这项工作将使 AM 人员 证监会将优化新设施的使用，为即将开展的大量具体项目做好准备 支持。

The overall goal of the AM SFC is to provide in one location the specialized facilities, equipment, and expertise to conduct research on BSL3 and Select Agent pathogens in animal models, which will promote the development of new vaccines, therapeutics, and other biodefense products for zoonotic agents. More specifically, the aims of the AM SFC are to establish and exploit for biodefense product development: 1. Facilities and expertise for aerosol inhalation studies using viral and bacterial pathogens that require high levels of containment 2. Facilities and expertise for studies involving arthropod-borne transmission using rodents as well as larger domestic animals and wildlife 3. Training to graduate and post-doctoral students, technicians and faculty members for development and implementation of animal model studies using aerosol and vector-borne transmission Personnel within the Animal Model Core will provide services to investigators at multiple levels. In some cases, personnel in the core will assist substantively in experimental design and be responsible for all aspects of animal experimentation, including delivery of the pathogen, monitoring and sampling (e.g. blood) from animals, and performing euthanasia and necropsies. This mode of service will be most applicable to investigators from other institutions and to projects involving private companies. The other end of the service spectrum will be to provide assistance as needed, especially in regard to aerosol and vector-borne delivery of pathogens. During the first year of the project, we will establish and characterize aerosol models in mice of three pathogens: the LVS strain of Francisella tularensis, LaCross encephalitis virus and rabies virus. These three pathogens are all models for important BT agents, and this work will allow personnel in the AM SFC to optimize the use of the new facility in preparation for the large number of specific projects that it will support.