Biosafety Level 3 (BSL3) Animal and Tissue Culture

生物安全 3 级 (BSL3) 动物和组织培养

• 批准号: 7645405
• 负责人: ANDREW B. ONDERDONK
• 金额: $ 87.07万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7645405
• 关键词: Address; Animal Housing; Animals; Biological; Caring; Categories; Containment; Core Facility; Ensure; Environment; Equipment; Event; Human Resources; Immunization; Laboratories; Laboratory Animals; Laboratory Research; Left; Life; MMP11 gene; Mission; New England; Numbers; Personnel Management; Physical environment; Quality Control; Research; Research Infrastructure; Research Personnel; Resources; Scientist; Secure; Security; Services; Technical Expertise; Toxin; Training; Work; animal tissue; biodefense; biosafety level 3 facility; cost effective; investigator training; pathogen; research study; tissue culture

The Biosafety Level 3 (BSL-3) Animal and Tissue Culture Core facility will provide the physical environment, equipment, and ancillary support personnel for scientists to work with Category A, B and C pathogens or toxins as part of biodefense research initiatives. The core facility will include both laboratory space and animal housing within a single containment facility. The facility will provide security required for possessing these select agents. It is the intent of this core facility to provide sufficient laboratory and animal capability so that any work with biological materials containing live pathogens can be performed within the facility. The objectives for the BSL-3 Core are: 1) to provide immediate access to secure BSL-3 laboratory and animal housing for regional NERCE scientists and to provide training for personnel handling category A, B and C agents; 2) to provide trained scientific staff to support investigators in their biodefense related research involving category A, B, and C agents; 3) to provide the infrastructure, administrative support and personnel for management of a secure BSL-3 facility; 4) to train NERCE scientists and technical personnel in the proper use of BSL-3 facilities; 5) to serve as a resource for local, State and Federal agencies in the event of a regional release of hazardous biologic agents or toxins. The Core will provide a number of services that are essential for work with category A agents and that will enhance the ability of investigators from New England to conduct productive research on these pathogens and their toxins. The services provided include: 1) a securerepository of characterized stocks of category A select agents; 2) propagation of working stocks of these pathogens; 3) laboratory animals for immunization and challenge experiments requiring BSL-3 containment; 4) equipment and technical expertise to conduct immunological analyses without removing biological materials generated in challenged animals from a BSL-3 environment; 5) quality control of biological materials to be removed from the BSL-3 facility to ensure that these materials do not contain viable pathogen.

生物安全3级(BSL-3)动物和组织培养核心设施将提供物理 科学家从事A、B和C类工作的环境、设备和辅助支持人员 病原体或毒素作为生物防御研究计划的一部分。核心设施将包括两个实验室 在一个隔离设施内的空间和动物住所。该设施将提供所需的安全保障 拥有这些精挑细选的特工。这个核心设施的目的是提供足够的实验室和动物 功能，以便使用包含活病原体的生物材料的任何工作都可以在 设施。BSL-3核心的目标是：1)提供对安全BSL-3实验室的即时访问 为地区NERCE科学家提供动物收容所，并为A类操作人员提供培训， B和C特工；2)提供训练有素的科学工作人员，支持调查人员与生物防御有关的工作 涉及A、B和C类代理的研究；3)提供基础设施、行政支持和 管理安全的BSL-3设施的人员；4)培训NERCE科学家和技术人员 正确使用BSL-3设施；5)作为地方、州和联邦机构在 危险生物制剂或毒素的区域性释放事件。核心将提供多项 与A类特工合作必不可少的服务，这些服务将增强调查人员的能力 对这些病原体及其毒素进行富有成效的研究。这些服务 提供包括：1)A类特定代理的特征股票的安全储存库；2)传播 这些病原体的工作种群；3)用于免疫和挑战实验的实验室动物 需要BSL-3遏制；4)进行免疫学分析的设备和技术专长 不从BSL-3环境中移除受挑战的动物产生的生物材料；5) 从BSL-3设施中移除生物材料的质量控制，以确保这些材料 不含活的病原体。