Structure and Function of ADAMTS13 Metalloprotease

ADAMTS13 金属蛋白酶的结构和功能

• 批准号: 7540945
• 负责人: X. Long Zheng
• 金额: $ 31.48万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7540945
• 关键词: ADAMTS; Acids; Active Sites; Address; Adult; Affinity; Alanine; Anabolism; Apical; Aspartic Acid; Autoantibodies; Basolateral Sorting Signal; Binding; Binding Sites; Biological; Biological Assay; Blood Circulation; Cellular Morphology; Chimeric Proteins; Cleaved cell; Clinical; Deletion Mutagenesis; Disintegrin Domain; Dissociation; Enzymes; Exhibits; Genes; Glutamine; Goals; Green Fluorescent Proteins; Hemostatic function; Idiopathic Thrombocytopenic Purpura; Immunoassay; Indium; Integrin Binding; Kinetics; Label; Length; Link; MDCK cell; Macroglobulins; Metalloproteases; Mutagenesis; Oligosaccharides; Outcome; Patients; Peptide Hydrolases; Peptides; Plasma; Plasma Exchange; Point Mutation; Prevention; Proteins; Radiolabeled; Recombinants; Regulation; Research; Research Personnel; Role; Signal Transduction; Site; Sorting - Cell Movement; Specificity; Structure; Surface Plasmon Resonance; Syndrome; Thrombocytopenic Purpura; Thrombosis; Thrombospondin 1; Thrombotic Thrombocytopenic Purpura; Time; Western Blotting; Zinc; analog; base; domain mapping; mutant; programs; radioligand; radiotracer; research study; tool; von Willebrand Factor

DESCRIPTION (provided by applicant): The catastrophic complications due to deficiency in plasma metalloprotease (ADAMTS13) highlight the biological importance of this protease in regulation of hemostasis and prevention of thrombosis in microvascular circulation that occurs in the thrombotic thrombocytopenic purpura (TTP) syndrome. Identification of the gene encoding ADAMTS13 metalloprotease and determination of the structure elements in the encoded protein have opened a new era in understanding of the roles of a zinc metalloprotease in regulation of hemostasis and thrombosis, and provided the essential tools to address the fundamental (patho)biological questions concerning ADAMTS13 including its biosynthesis, activation, substrate recognition, intracellular sorting and autoantibody interaction. The goals of the proposed research are: Specific Aim 1. To determine ADAMTS13 substrate recognition by 1) analyzing the proteolytic activity of wild type ADAMTS13 and various mutants of ADAMTS13 toward von Willebrand factor and its analog, VWF73; 2) determining the binding kinetics between VWF or VWF73 and wild type or mutant ADAMTS13 by radioligand binding and surface plasmon resonance assays. Specific Aim 2. To determine intracellular sorting of ADAMTS13 by 1) determining the polarity of full-length and mutant ADAMTS13 secretion in MDCK cells; 2) determining the sorting signal and mechanisms by which ADAMTS13 is sorted; 3) determining the effect of point mutations or truncations of ADAMTS13 gene found in patients with congenital TTP on the polarity of ADAMTS13 secretion. Specific Aim 3. To determine the domains (or sites) of ADATMTS13 to which anti-ADAMTS13 autoantibodies bind and the kinetic parameters (affinity and specificity) of interaction between ADAMTS13 metalloprotease and anti-ADAMTS13 autoantibodies, and to correlate the clinical course and outcome to the distinct type of anti-ADAMTS autoantibodies identified in patients with TTP.

描述(由申请人提供)：由于血浆金属蛋白酶缺乏引起的灾难性并发症(ADAMTS13)突出了这种酶在调节血栓性血小板减少性紫癜(TTP)综合征中的止血和预防微血管循环中血栓形成方面的生物学重要性。ADAMTS13金属蛋白酶编码基因的鉴定和编码蛋白中结构元素的测定为理解锌金属蛋白酶在止血和血栓形成中的作用开辟了一个新的时代，并为解决ADAMTS13的生物合成、激活、底物识别、细胞内分选和自身抗体相互作用等基本生物学问题提供了必要的工具。本研究的目标是：1.通过分析野生型ADAMTS13及其不同突变体对von Willebrand因子及其类似物VWF73的蛋白降解活性，确定ADAMTS13底物的识别能力；2)通过放射性配基结合和表面等离子体共振分析，确定VWF或VWF73与野生型或突变型ADAMTS13的结合动力学。确定ADAMTS13的细胞内分选：1)确定MDCK细胞中全长和突变的ADAMTS13分泌的极性；2)确定ADAMTS13的分选信号和分选机制；3)确定先天性TTP患者中发现的ADAMTS13基因点突变或截短对ADAMTS13分泌的极性的影响。具体目的3.确定抗ADAMTS13自身抗体与ADATMTS13结合的区域(或部位)以及ADAMTS13金属蛋白酶与抗ADAMTS13自身抗体相互作用的动力学参数(亲和力和特异性)，并将TTP患者的临床病程和转归与不同类型的抗ADAMTS13自身抗体相关联。

项目成果

A team player: the disintegrin domain of ADAMTS13.

团队合作者：ADAMTS13 的解整合素结构域。

• DOI: 10.1182/blood-2009-03-211300
• 发表时间: 2009
• 期刊: Blood
• 影响因子: 20.3
• 作者: Zheng,XLong

Arsenic trioxide induces apoptosis in B-cell chronic lymphocytic leukemic cells through down-regulation of survivin via the p53-dependent signaling pathway.

• DOI: 10.1016/j.leukres.2013.09.019
• 发表时间: 2013-12
• 期刊: Leukemia research
• 影响因子: 2.7
• 作者: Zhang XH;Feng R;Lv M;Jiang Q;Zhu HH;Qing YZ;Bao JL;Huang XJ;Zheng XL
• 通讯作者: Zheng XL

Thromboelastography of patients after fontan compared with healthy children.

Fontan 治疗后患者与健康儿童的血栓弹力图比较。

• DOI: 10.1007/s00246-009-9434-1
• 发表时间: 2009
• 期刊: Pediatric cardiology
• 影响因子: 1.6
• 作者: Raffini,Leslie;Schwed,Alexander;Zheng,XLong;Tanzer,Maria;Nicolson,Susan;Gaynor,JWilliam;Jobes,David
• 通讯作者: Jobes,David