EFFECTS OF LEUCINE ENHANCED AMINO ACID DRINK ON MUSCLE PROTEIN METABOLISM IN THE

亮氨酸增强氨基酸饮料对肌肉蛋白质代谢的影响

• 批准号: 7605384
• 负责人: ROBERT R WOLFE
• 金额: $ 1.49万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605384
• 关键词: Activities of Daily Living; Adult; Age; Amino Acids; Biopsy; Cardiovascular system; Computer Retrieval of Information on Scientific Projects Database; Data; Elderly; Essential Amino Acids; Female; Fracture; Funding; Goals; Grant; Hour; Individual; Ingestion; Institution; Investigation; Kinetics; Leucine; Metabolic; Methodology; Muscle; Muscle Proteins; Patients; Peripheral Vascular Diseases; Protein Biosynthesis; Research; Research Personnel; Resources; Risk; Sampling; Skeletal Muscle; Source; Supplementation; Tissues; Tracer; United States National Institutes of Health; Venous; Weight; day; drinking; experience; falls; male; protein metabolism; research study; sarcopenia; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many adults experience a progressive loss of skeletal muscle mass with increasing age. This concomitant loss of contractile tissue and functional capacity (i.e., sarcopenia) contributes to increased risk of falls, bone fracture and may substantially impair many activities of daily living. Given the fact that net muscle protein synthesis is a major metabolic determinant of muscular strength and functional capacity, the goal of this study is to determine if a leucine enhanced amino acid drink will stimulate net muscle protein synthesis to a greater extent compared to an equivalent (same weight in terms of amino acid composition) amino acid drink containing only the essential amino acids. The direct comparison of those two drinks will provide new information on the effects of including a larger amount of leucine (compared to the other essential amino acids) in a drink, which will serve as important preliminary data for subsequent investigations looking at optimizing amino acid supplementation in elderly individuals. To achieve the goals of this research, a total of 8 healthy patients with no prior cardiovascular or peripheral vascular disease will participate in this research study. Subjects will be normal healthy males and females between the ages of 60 and 85. The study will consist of two 8-hour trials performed on separate days. The study will examine muscle protein kinetics prior to and following the ingestion of the amino acids containing drinks. Muscle protein kinetics will be determined using established stable isotope tracer methodology, arterial-venous sampling and muscle biopsies.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 许多成年人随着年龄的增长经历骨骼肌质量的进行性损失。这种伴随的收缩组织和功能能力的丧失（即，肌肉减少症）导致福尔斯、骨折的风险增加，并可能严重损害许多日常生活活动。 考虑到净肌肉蛋白质合成是肌肉力量和功能能力的主要代谢决定因素，本研究的目的是确定与仅含有必需氨基酸的同等（氨基酸组成方面相同重量）氨基酸饮料相比，亮氨酸增强的氨基酸饮料是否会在更大程度上刺激净肌肉蛋白质合成。这两种饮料的直接比较将提供有关饮料中含有大量亮氨酸（与其他必需氨基酸相比）的影响的新信息，这将作为后续研究的重要初步数据，以优化老年人的氨基酸补充。为了实现本研究的目标，共有8例既往无心血管或外周血管疾病的健康患者将参与本研究。受试者将是年龄在60至85岁之间的正常健康男性和女性。本研究将包括在不同日期进行的两项8小时试验。这项研究将检查肌肉蛋白动力学之前和之后的氨基酸含有饮料的摄入。肌肉蛋白动力学将使用已建立的稳定同位素示踪剂方法、动脉-静脉取样和肌肉活检来确定。