APOLIPOPROTEIN-BASED LIPOPROTEIN SUBCLASS PROFILES & VASC COMPLIC OF DIABETES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives: To investigate apolipoprotein-based lipoprotein subclass profiles in diabetic and control subjects and their associations with diabetic retinopathy (DR) in Type 1 diabetes and the effect of statins in Type 2 diabetes. Rationale: Vascular complications cause a majority of the morbidity and mortality of diabetes. Evidence suggests that dyslipidemia mediates risk for micro- and macro-vascular complications. There are no reports about the characterization of apolipoprotein-defined lipoprotein subclasses in Type 1 diabetes in relation to DR, and inadequate information about effects of commonly used lipid lowering drugs on subclasses in Type 2 diabetes. Since apolipoprotein-based particle analysis allows a new view of a complex system, this proposal will provide important new knowledge. Hypotheses: Apolipoprotein-based plasma lipoprotein subclass profiles are associated with the development of the macro- and micro-vascular complications of diabetes, and may help identify at-risk patients for vascular complications. Specific Aims: Determine apolipoprotein-based lipoprotein profile in (Aim 1) 50 type 1 diabetic patients (25 with DR, 25 without DR), and in control subjects to define associations with retinopathy and diabetes; and (Aim 2) in 50 type 2 diabetic patients (25 taking statins, 25 not taking statins), and healthy control subjects and to define associations with statin consumption. Aim 3: Investigate relationship between apolipoprotein-based profiles in type 1 and type 2 diabetic subjects and other lipoprotein characteristics (NMR, apoA1, B and Lp (a), conventional lipid profiles). Methods: Patients will be recruited from the patient cohort attending our diabetes clinics. All methods for apolipoprotein subclass determination and data analysis are in place.