Genetic Control of Entry into Meiosis in Yeast

酵母进入减数分裂的遗传控制

• 批准号: 7596442
• 负责人: AARON P MITCHELL
• 金额: $ 36.53万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596442
• 关键词: Binding Sites; Biological; Biological Assay; Biological Phenomena; Candida albicans; Cell membrane; Cells; Cessation of life; Communicable Diseases; Complex; Culture Media; Defect; Diagnostic; Disease; Dissection; Endocytic Vesicle; Endocytosis; Endothelial Cells; Gene Targeting; Genes; Genetic; Growth; Homologous Gene; In Vitro; Infection; Measurement; Meiosis; Membrane Proteins; Metabolism; Methods; Microarray Analysis; Modeling; Nature; Pathogenicity; Pathway interactions; Process; Proteins; Public Health; Research Personnel; Role; Saccharomyces cerevisiae; Saccharomycetales; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Site; Surveys; Testing; Transcriptional Activation; Virulence; Virulence Factors; Work; Yeasts; Zinc Fingers; cell injury; environmental adaptation; gene function; insight; interest; microbial; mutant; novel; novel therapeutics; pathogen; response; transcription factor

DESCRIPTION (provided by applicant): Environmental response pathways are of interest as models for conserved signal transduction pathways and as determinants of virulence among microbial pathogens. Our studies have focused on the mechanisms through which yeast cells sense and respond to changes in external pH. Two pathways that are required for growth of budding yeast Saccharomyces cerevisiae at alkaline pH and whose homologs are required for full virulence of the fungal pathogen Candida albicans: the Rim101p pathway and the MdsSp pathway. Our broad objective is to understand, for each pathway, the specific signal that is recognized, the mechanism of signal transduction, and the target genes and functions that contribute to pathogenicity. Our experimental methods employ mainly genetic and cell biological methods. Our specific aims are to identify the signal that governs Rim101p pathway activity, to determine the relationship of Mds3p/Pmd1p to transcriptional targets, and to determine the roles of C. albicans Rim101p and MdsSp target genes in virulence. Our work is relevant to public health in providing insight into how an infectious disease agent is able to grow in so many different body sites, and how it damages our cells to cause disease and death.

描述（由申请人提供）：环境响应途径作为保守信号转导途径的模型和微生物病原体毒力的决定因素而受到关注。我们的研究重点是酵母细胞感知和响应外部 pH 变化的机制。酿酒酵母在碱性 pH 下生长所需的两条途径，以及真菌病原体白色念珠菌完全毒力所需的同系物：Rim101p 途径和 MdsSp 途径。我们的总体目标是了解每种途径所识别的特定信号、信号转导机制以及导致致病性的靶基因和功能。我们的实验方法主要采用遗传和细胞生物学方法。我们的具体目标是识别控制 Rim101p 通路活性的信号，确定 Mds3p/Pmd1p 与转录靶标的关系，并确定白色念珠菌 Rim101p 和 MdsSp 靶基因在毒力中的作用。我们的工作与公共卫生相关，深入了解传染病病原体如何在如此多的身体不同部位生长，以及它如何损害我们的细胞导致疾病和死亡。