Genetic Control of Eye Specification

描述（由申请人提供）：本提案的长期目标是详细了解果蝇眼组织决定的遗传控制。眼原基的特化涉及多个信号传导途径（dpp、wg、hh）和转录因子（Toy、Ey、Eya、So、Dae、Hth、Tsh）之间的相互作用，所述多个信号传导途径和转录因子以控制眼神经发生起始的调节级联连接。在这项研究中，我们建议研究这些分子在启动神经发生中的功能，分析一种新基因在这一过程中的作用，并确定其他有助于眼睛特异性的因素。 这项赠款的具体目标是： 1.通过解剖前神经基因的转录调控，阐明神经发生启动的分子机制; 2.在分子和遗传水平上研究眼特化所需位点1（3）96 Bb-CG 5807的功能; 3.以确定新的监管机构的眼睛规格依赖于一个修改的表达错误的屏幕。 由于本提案中研究的因素是保守的，并在脊椎动物眼睛发育中发挥重要作用，我们的工作将提供对哺乳动物眼睛发育至关重要的遗传因素的见解，并与眼睛和大脑的先天性疾病有关。

DESCRIPTION (provided by applicant): The long-term objective of this proposal is to gain a detailed understanding of the genetic control of eye-tissue determination in Drosophila. The specification of the eye primordium involves interactions between multiple signaling pathways (dpp, wg, hh) and transcription factors (Toy, Ey, Eya, So, Dae, Hth, Tsh) linked in a regulatory cascade that controls initiation of neurogenesis in the eye. In this grant, we propose to investigate the function of these molecules in initiating neurogenesis, to analyze the role of a novel gene in this process, and to identify other factors that contribute to eye specification. The specific aims of this grant are: 1. to elucidate the molecular mechanisms leading to initiation of neurogenesis by dissecting the transcriptional control of the proneural gene atonal; 2. to investigate at molecular and genetic levels the function of l(3)96Bb-CG5807, a locus required for eye specification; 3. to identify novel regulators of eye specification relying on a modified misexpression screen. Since the factors studied in this proposal are conserved and play significant roles in vertebrate eye development, our work will provide insights into genetic factors that are central to mammalian eye development and are linked to congenital disorders of the eye and brain.