Role of estrogen and its receptors in ovarian cancer of the hen

项目概要： 卵巢癌是妇女癌症死亡的第五大原因。据了解，有一个 仅接受雌激素替代疗法的女性患卵巢癌的风险增加， 服用避孕药的女性的风险可能会降低，这支持了类固醇的观点 激素在卵巢癌的病因学中起作用。本项目旨在阐明 雌激素及其受体与母鸡卵巢癌的关系母鸡是一个很好的模型，因为它 卵巢癌的自发发病率很高。本研究的具体目的是：1） 确定母鸡的卵巢癌是否是雌激素依赖性的; 2）确定雌激素受体是否是 在卵巢癌中差异调节; 3）表征非核雌激素受体;和4） 确定与雌激素受体介导的增殖相关的下游途径。这些 将使用多种分子技术回答问题，包括PCR，Western印迹， 免疫组织化学和微阵列分析，体外方法，包括细胞培养，离体（或卵内） 方法包括正常和肿瘤卵巢组织的CAM移植物，以及体内方法包括治疗 雌激素的作用。一旦这些问题得到解答，我们就会对角色有更好的理解 雌激素及其受体在卵巢癌中的作用这些答案也将使我们了解 雌激素促进母鸡卵巢肿瘤形成或增殖的作用机制， 可能与雌激素及其受体M、Ay在人卵巢癌中的作用有关。此外，委员会认为， 了解雌激素的作用可能会导致可能适用于人类的治疗选择， 疾病 相关性： 卵巢癌是妇女癌症死亡的第五大原因。雌激素可促进 卵巢肿瘤的发生或增殖;因此，重要的是要了解其在起源或 疾病的进展。

Project summary: Ovarian cancer is the fifth leading cause of cancer death among women. It is known that there is an increased risk of ovarian cancer in women who are on estrogen-only hormone replacement therapy and possible decreased risk in women who take the contraceptive pill, lending support to the idea that steroid hormones are playing a role in the etiology of ovarian cancer. This project is designed to clarify the role of estrogen and its receptors in ovarian cancer of the domestic hen. The hen is a good model because it develops ovarian cancer with a high spontaneous incidence. The specific aims of this study are to: 1) determine whether ovarian cancer in the hen is estrogen dependent; 2) determine if the estrogen receptor is differentially regulated in ovarian cancer; 3) characterize the non-nuclear estrogen receptor; and 4) determine downstream pathways associated with estrogen receptor mediated proliferation. These questions will be answered using a variety of molecular techniques including PCR, Western blot, immunohistochemistry and microarray analyses, in vitro methods including cell culture, ex vivo (or in ovo) methods including CAM grafts of normal and tumor ovarian tissue, and in vivo methods including treatment of hens with estradiol. Once these questions are answered, we will have a better understanding of the role of estrogen and its receptors in ovarian cancer. These answers will also allow us to understand the mechanism of estrogen action promoting the formation or proliferation of ovarian tumors in the hen, which may be correlated to the role estrogen and its receptors m,ay play in human ovarian cancer. Furthermore, understanding estrogen's actions might lead to possible treatment options that could be applicable to human disease. Relevance: Ovarian cancer is the fifth leading cause of cancer death among women. Estrogens may promote ovarian tumor initiation or proliferation; therefore, it is important to understand its role in the origin or progression of the disease.