Image Slicing Spectrometer (ISS) for high resolution sub-cellular microscopy

用于高分辨率亚细胞显微镜的图像切片光谱仪 (ISS)

基本信息

  • 批准号:
    7695567
  • 负责人:
  • 金额:
    $ 18.55万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-09-30 至 2011-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal is directed toward development of a fluorescent spectral imaging system for simultaneous high resolution sub-cellular microscopy of multiple fluorescence probes in living cells. Recent developments in fluorescent probes, imaging instrumentation and micro- fabrication now permit building for an Image Slicing Spectrometer (ISS) for real time quantitative spectral imaging. We propose to combine our expertise in microscopy, optical design, fabrication, and imaging with newly available large format CCD cameras and fabrication techniques to develop an ISS system. ISS is a widefield method that is capable of acquiring full spectral information simultaneously from every pixel. This approach works by spatially redirecting image zones to obtain space between image lines. Next, by using a diffractive element, ISS obtains wavelength spread on the CCD camera (for more details on the system principle see Sections C and D). In this way, we unambiguously map x,y, ?; data onto the 2-D image sensor. The specific aims of the project are: (1) to construct the ISS with an initial wavelength range of 450 to 700 nm and (2) to test the Image Slicing Spectrometer against currently available spectral imaging systems in several live cell imaging applications. Work on development of imaging spectrometers for cellular imaging has thus far been hampered either by small fields of view, limited temporal-spatial-spectral resolution, requirement of extensive computations, or limited light efficiency. The Image Slicing Spectrometer proposed here is based on a concept borrowed from the astronomy field, and addresses the major difficulties previously connected with construction of a snapshot spectrometer. The Image Slicer transforms a rectangular Field of View (FoV) into a series of mini "slits", and rearranges them to create sufficient area for spectral spread and acquisition in the snapshot mode. No complicated processing is necessary and only simple remapping is sufficient to obtain a complete x,y, ?; data cube. The core of the system will be a custom-made redirecting mirror fabricated with diamond turning technology. The instrument will employ a Hamamatsu CCD camera with 4000 x 2624 pixel elements, Peltier cooling, and low-noise readout (C4742-98-24HR). Using this large format CCD, the final image data cube will be 400 x 260 x 50 (X, Y, ?) with a spectral resolution of 5 nm and ~0.5 < ¿m spatial resolution. Once the system is built and optimized we will quantitatively evaluate the results from the ISS against the Zeiss LSM510 META, and an Optical Insights Spectral DV system. These evaluations will utilize "standard" fluorophore combinations, starting with two-color pairs, but including more challenging combinations such as CFP/GFP/YFP/Fluo-4, and mCherry/SNARF-1/Fura-Red. In summary the ISS has the potential to significantly advance a wide range of applications in area of cellular imaging. To further its impact, we plan to combine the ISS with optical sectioning in the future, using structured illumination, Nipkow disk confocal, and/or spatial deconvolution. Although it is beyond the scope of the present application, a 4-dimensional imaging system (X, Y, ?, ;) would further improve the S/N of the data, as well as speed of 4-D imaging. The project targets the development of a modern spectrometer called Image Slicing Spectrometer enabling high resolution spectral imaging in real time. In consequence researchers will be able to rapidly advance the investigation of live cells with multiple fluorescent contrasts. The instrument's principle allows obtaining spectral information for entire image without scanning and thus improve signal to noise ratio. It also allows also efficient investigation of transient biological events. Technologies applied in the project and their low cost may potentially allow access of larger group of scientists to spectral imaging instrumentation.
描述(由申请人提供):本提案针对开发用于活细胞中多个荧光探针的同时高分辨率亚细胞显微镜检查的荧光光谱成像系统。荧光探针、成像仪器和微加工的最新发展现在允许构建用于真实的定量光谱成像的图像切片光谱仪(ISS)。我们建议联合收割机结合我们在显微镜,光学设计,制造和成像与新的大幅面CCD相机和制造技术,开发一个国际空间站系统的专业知识。国际空间站是一种宽视场方法,能够从每个像素同时获取全部光谱信息。该方法通过在空间上重定向图像区域以获得图像线之间的空间来工作。接下来,通过使用衍射元件,ISS获得CCD相机上的波长扩展(有关系统原理的更多详细信息,请参见C和D部分)。用这种方法,我们明确地映射x,y,?将数据传输到二维图像传感器上。该项目的具体目标是:(1)建造初始波长范围为450至700纳米的国际空间站;(2)在若干活细胞成像应用中,对照现有光谱成像系统测试图像切片光谱仪。用于细胞成像的成像光谱仪的开发工作迄今为止受到小视场、有限的时间-空间-光谱分辨率、大量计算的要求或有限的光效率的阻碍。这里提出的图像切片光谱仪是基于从天文学领域借用的概念,并解决了以前与快照光谱仪的建设有关的主要困难。图像切片器将矩形视场(FoV)转换为一系列微型“狭缝”,并重新排列它们,以创建足够的区域,用于在快照模式下进行光谱扩展和采集。不需要复杂的处理,只需简单的重映射就足以获得完整的x,y,?数据立方体该系统的核心将是一个定制的重定向反射镜与金刚石车削技术制造。该仪器将采用具有4000 x 2624像素元件、Peltier冷却和低噪声读数的Hamamatsu CCD相机(C4742-98- 24 HR)。使用这种大幅面CCD,最终的图像数据立方体将是400 x 260 x 50(X,Y,?)具有5 nm的光谱分辨率和~0.5 m的空间分辨率。一旦系统建成并优化,我们将根据蔡司LSM 510 Meta和Optical Insights Spectral DV系统对ISS的结果进行定量评估。这些评估将使用“标准”荧光团组合,从双色对开始,但包括更具挑战性的组合,如CFP/GFP/YFP/Fluo-4和mCherry/SNARF-1/Fura-Red。总之,ISS具有显著推进细胞成像领域广泛应用的潜力。为了进一步发挥其影响,我们计划在未来将国际空间站与光学切片相结合,使用结构化照明,Nipkow盘共焦和/或空间解卷积。虽然超出了本申请的范围,但是可以使用4维成像系统(X,Y,?,)将进一步提高数据的S/N以及4-D成像的速度。该项目的目标是开发一种名为图像切片光谱仪的现代光谱仪,实现真实的高分辨率光谱成像。因此,研究人员将能够快速推进对具有多种荧光对比的活细胞的研究。该仪器的原理允许在不扫描的情况下获得整个图像的光谱信息,从而提高信噪比。它还允许对瞬时生物事件的有效调查。该项目中采用的技术及其低成本可能使更多的科学家能够使用光谱成像仪器。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Snapshot hyperspectral retinal camera with the Image Mapping Spectrometer (IMS).
  • DOI:
    10.1364/boe.3.000048
  • 发表时间:
    2012-01-01
  • 期刊:
  • 影响因子:
    3.4
  • 作者:
    Gao L;Smith RT;Tkaczyk TS
  • 通讯作者:
    Tkaczyk TS
Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy.
  • DOI:
    10.1364/oe.17.012293
  • 发表时间:
    2009-07-20
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Gao L;Kester RT;Tkaczyk TS
  • 通讯作者:
    Tkaczyk TS
Correction of vignetting and distortion errors induced by two-axis light beam steering.
Depth-resolved image mapping spectrometer (IMS) with structured illumination.
深度分辨图像映射光谱仪(IMS)带有结构化照明。
  • DOI:
    10.1364/oe.19.017439
  • 发表时间:
    2011-08-29
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Gao L;Bedard N;Hagen N;Kester RT;Tkaczyk TS
  • 通讯作者:
    Tkaczyk TS
Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.
  • DOI:
    10.1111/j.1365-2818.2012.03596.x
  • 发表时间:
    2012-05
  • 期刊:
  • 影响因子:
    2
  • 作者:
    Gao L;Hagen N;Tkaczyk TS
  • 通讯作者:
    Tkaczyk TS
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TOMASZ S TKACZYK其他文献

TOMASZ S TKACZYK的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TOMASZ S TKACZYK', 18)}}的其他基金

Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging
用于分子成像的光导图像处理 (LIP) 3D 打印快照光谱仪
  • 批准号:
    10447267
  • 财政年份:
    2022
  • 资助金额:
    $ 18.55万
  • 项目类别:
Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging
用于分子成像的光导图像处理 (LIP) 3D 打印快照光谱仪
  • 批准号:
    10613348
  • 财政年份:
    2022
  • 资助金额:
    $ 18.55万
  • 项目类别:
Imaging CAncer Screening Patch (CASP) for early diagnostics of cervical cancers
用于宫颈癌早期诊断的成像癌筛查贴片 (CASP)
  • 批准号:
    9316232
  • 财政年份:
    2017
  • 资助金额:
    $ 18.55万
  • 项目类别:
Adaptive Miniature Microscopy Platform for High Throughput Biological Imaging
用于高通量生物成像的自适应微型显微镜平台
  • 批准号:
    8840586
  • 财政年份:
    2014
  • 资助金额:
    $ 18.55万
  • 项目类别:
Adaptive Miniature Microscopy Platform for High Throughput Biological Imaging
用于高通量生物成像的自适应微型显微镜平台
  • 批准号:
    8638575
  • 财政年份:
    2014
  • 资助金额:
    $ 18.55万
  • 项目类别:
Miniature Foveated Endoscope for Early Cancer Diagnostics
用于早期癌症诊断的微型中央凹内窥镜
  • 批准号:
    8549922
  • 财政年份:
    2012
  • 资助金额:
    $ 18.55万
  • 项目类别:
Miniature Foveated Endoscope for Early Cancer Diagnostics
用于早期癌症诊断的微型中央凹内窥镜
  • 批准号:
    8445549
  • 财政年份:
    2012
  • 资助金额:
    $ 18.55万
  • 项目类别:
Image Slicing Spectrometer (ISS) for high resolution sub-cellular microscopy
用于高分辨率亚细胞显微镜的图像切片光谱仪 (ISS)
  • 批准号:
    7572583
  • 财政年份:
    2008
  • 资助金额:
    $ 18.55万
  • 项目类别:
Integrated Bi-FOV Endoscope for Detection of Precancer
用于检测癌前病变的集成双视场内窥镜
  • 批准号:
    7661361
  • 财政年份:
    2007
  • 资助金额:
    $ 18.55万
  • 项目类别:
Integrated Bi-FOV Endoscope for Detection of Precancer
用于检测癌前病变的集成双视场内窥镜
  • 批准号:
    8119400
  • 财政年份:
    2007
  • 资助金额:
    $ 18.55万
  • 项目类别:

相似海外基金

Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
  • 批准号:
    MR/S03398X/2
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
  • 批准号:
    EP/Y001486/1
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
  • 批准号:
    2338423
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
  • 批准号:
    MR/X03657X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
  • 批准号:
    2348066
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Standard Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
  • 批准号:
    2341402
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
  • 批准号:
    AH/Z505481/1
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10107647
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    EU-Funded
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10106221
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
  • 批准号:
    AH/Z505341/1
  • 财政年份:
    2024
  • 资助金额:
    $ 18.55万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了