SMALL ANGLE X-RAY SCATTERING STUDIES ON ACTIN BUNDLES

肌动蛋白束的小角 X 射线散射研究

• 批准号: 7598206
• 负责人: PAUL T. MATSUDAIRA
• 金额: $ 0.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598206
• 关键词: Actins; C-terminal; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Institution; Measurement; Measures; Positioning Attribute; Range; Relative (related person); Research; Research Personnel; Resources; Solutions; Source; United States National Institutes of Health; detector; radius bone structure; scruin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. From the spread or variability in the radius of gyration of scruin, we will measure the range in distances that separate the N-and C-terminal beta-propeller domains from the spread in the radius of gyration. Because scruin controls actin twist in the acrosomal bundle, the relative positions of the domains will give an upper boundry on the strain that can be stored in the twist of actin. We have previously collected solution scattering data to measure the Rg as a function of pCa. However, we cannot extract the variability in the Rg to the accuracy that we need. With improvements to the detector, we can re-collect the data and extract the measurements.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 根据SCORIN回转半径的扩散或可变性，我们将测量N-末端和C-末端的β-螺旋桨区域与回转半径的扩散的距离。因为细菌素控制着顶体束中肌动蛋白的扭转，这些区域的相对位置将给出一个可以储存在肌动蛋白扭转中的应变的上界。我们以前收集了溶液散射数据来测量Rg作为PCA的函数。然而，我们不能将RG中的可变性提取到我们需要的准确性。通过对探测器的改进，我们可以重新收集数据并提取测量结果。