FEI TECNAI 12 SPIRIT BIO TWIN: NANOTECHNOLOGY

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：怀特黑德研究所申请500,000美元购买一台FEI Technai 12 SPIRIT BioTwin TEM，安装在WM Keck显微镜设施中。FEI的瞬变电磁波将取代已有20年历史的菲利普斯410型瞬变电磁波，从而提升该设施的瞬变电磁波服务。410的使用寿命即将结束，维护变得越来越困难。更重要的是，新一代FEI TEM为用户提供半自动操作和基于数字CCD的成像速度，并为用户和瞬变技术人员简化图像捕获、处理和分析。作为岩心显微镜设备中的一种仪器，透射电子显微镜被世界范围内的研究人员广泛使用。此外，同一台电子显微镜是麻省理工学院校园内唯一通用的生物电子显微镜，服务于生物、生物工程、工程、化学和其他麻省理工学院的实验室。此外，来自波士顿地区的相当数量的研究人员也使用了瞬变电磁。因此，一个升级的瞬变电磁将使一个相当大的研究基础受益。该提案描述了美国国立卫生研究院支持的六名研究人员和塔夫茨大学一名新的助理教授的研究，塔夫茨大学没有生物电子显微镜；为了支持FEI技术电子显微镜，WL承诺每年预算42,652美元，用于EM技术员的工资和维护合同。此外，WL将支付53,910美元用于购买、运输和支付显微镜的关税。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Whitehead Institute requests $500,000 for the purchase of a FEI Technai 12 Spirit BioTwin TEM to be housed in the WM Keck Microscope Facility. The FEI TEM will up-grade the TEM services of the facility by replacing a twenty-year old Phillips 410 TEM. The 410 is nearing the end of its operational lifetime and maintenance is becoming difficult. More importantly, new generation FEI TEMs provide semi-automated operations for users and digital CCD-based imaging speeds and simplifies image capture, processing, and analysis for users and TEM technical staff. As an instrument in a core microscope facility, the TEM is widely used by Wl researchers. In addition, the same TEM is the only general use biological TEM on the MIT campus and serves labs in biology, bioengineering, engineering, chemistry, and other MIT departments. Furthermore, a significant number of researchers from the Boston-area also use the TEM. Thus, an up- graded TEM would benefit a significantly large research base. The proposal describes the research of six NIH-supported researchers and one new assistant professor at Tufts U. which does not have a biological TEM; To support the FEI Technai TEM, the Wl is committing an annual budget of $42,652 for an EM technician salary and maintenance contract. Furthermore, the Wl will pay $53,910 toward the purchase, shipping, and duty of the microscope.