FEI TECNAI 12 SPIRIT BIO TWIN: NANOTECHNOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：怀特黑德研究所要求50万美元用于购买FEI Technai 12 Spirit BioTwin TEM，该TEM将被安置在WM Keck显微镜设施中。FEI TEM将通过更换使用了20年的菲利普斯410 TEM来升级该设施的TEM服务。410的使用寿命即将结束，维护变得越来越困难。更重要的是，新一代FEI TEM为用户提供了半自动化操作和基于CCD的数字成像速度，并简化了用户和TEM技术人员的图像捕获、处理和分析。TEM作为岩心显微镜设备中的一种仪器，被WI研究人员广泛使用。此外，同一个TEM是麻省理工学院校园内唯一的通用生物TEM，为生物学，生物工程，工程，化学和其他麻省理工学院部门的实验室提供服务。此外，来自波士顿地区的大量研究人员也使用TEM。因此，一个升级的TEM将有利于一个相当大的研究基地.该提案描述了六名NIH支持的研究人员和一名塔夫茨大学的新助理教授的研究。为了支持FEI Technai TEM，WI承诺每年为EM技术员工资和维护合同提供42，652美元的预算。此外，WI将支付53，910美元用于购买，运输和显微镜的关税。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Whitehead Institute requests $500,000 for the purchase of a FEI Technai 12 Spirit BioTwin TEM to be housed in the WM Keck Microscope Facility. The FEI TEM will up-grade the TEM services of the facility by replacing a twenty-year old Phillips 410 TEM. The 410 is nearing the end of its operational lifetime and maintenance is becoming difficult. More importantly, new generation FEI TEMs provide semi-automated operations for users and digital CCD-based imaging speeds and simplifies image capture, processing, and analysis for users and TEM technical staff. As an instrument in a core microscope facility, the TEM is widely used by Wl researchers. In addition, the same TEM is the only general use biological TEM on the MIT campus and serves labs in biology, bioengineering, engineering, chemistry, and other MIT departments. Furthermore, a significant number of researchers from the Boston-area also use the TEM. Thus, an up- graded TEM would benefit a significantly large research base. The proposal describes the research of six NIH-supported researchers and one new assistant professor at Tufts U. which does not have a biological TEM; To support the FEI Technai TEM, the Wl is committing an annual budget of $42,652 for an EM technician salary and maintenance contract. Furthermore, the Wl will pay $53,910 toward the purchase, shipping, and duty of the microscope.