FEI Tecnai 12 Spirit Bio Twin

描述（由申请人提供）：怀特黑德研究所申请50万美元用于购买FEI Technai 12 Spirit BioTwin TEM，用于WM Keck显微镜设施。FEI TEM将通过替换已有20年历史的Phillips 410 TEM来升级该设施的TEM服务。410已接近其使用寿命的终点，维护变得越来越困难。更重要的是，新一代FEI TEM为用户提供半自动化操作和基于数字ccd的成像速度，并简化了用户和TEM技术人员的图像捕获、处理和分析。作为核心显微镜设备中的一种仪器，瞬变电子显微镜被广泛应用于研究领域。此外，同一台TEM是麻省理工学院校园内唯一的通用生物TEM，为生物学、生物工程、工程、化学和其他麻省理工学院部门的实验室提供服务。此外，来自波士顿地区的大量研究人员也使用瞬变电磁法。因此，一个升级的瞬变电磁法将有利于一个显著的大型研究基地。该提案描述了六名美国国立卫生研究院支持的研究人员和塔夫茨大学一名新助理教授的研究，塔夫茨大学没有生物TEM；为了支持FEI Technai TEM， Wl承诺为EM技术人员的工资和维护合同提供42,652美元的年度预算。此外，Wl将支付53,910美元用于购买，运输和显微镜的关税。

DESCRIPTION (provided by applicant): The Whitehead Institute requests $500,000 for the purchase of a FEI Technai 12 Spirit BioTwin TEM to be housed in the WM Keck Microscope Facility. The FEI TEM will up-grade the TEM services of the facility by replacing a twenty-year old Phillips 410 TEM. The 410 is nearing the end of its operational lifetime and maintenance is becoming difficult. More importantly, new generation FEI TEMs provide semi-automated operations for users and digital CCD-based imaging speeds and simplifies image capture, processing, and analysis for users and TEM technical staff. As an instrument in a core microscope facility, the TEM is widely used by Wl researchers. In addition, the same TEM is the only general use biological TEM on the MIT campus and serves labs in biology, bioengineering, engineering, chemistry, and other MIT departments. Furthermore, a significant number of researchers from the Boston-area also use the TEM. Thus, an up- graded TEM would benefit a significantly large research base. The proposal describes the research of six NIH-supported researchers and one new assistant professor at Tufts U. which does not have a biological TEM; To support the FEI Technai TEM, the Wl is committing an annual budget of $42,652 for an EM technician salary and maintenance contract. Furthermore, the Wl will pay $53,910 toward the purchase, shipping, and duty of the microscope.