WORKSHOP ON SMALL-ANGLE X-RAY SCATTERING AND DIFFRACTION STUDIES IN STRUCTURAL B

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 SSRL SMB bio-SAXS/D团队于2006年7月28日至30日举办了一次实践研讨会。选择7月下旬的日期是为了包括6个光束时移的动手实验辅导课程，补充2005年10月的最后一次生物SAXS/D研讨会。研讨会的前半部分由特邀专家发言人口头介绍了结构生物学中主要是非晶体X射线衍射研究，从生物SAXS/D技术的基础知识到SSRL和其他地方进行的尖端先进应用。这些演讲包括对SAXS/D技术的结构生物学研究的全面回顾，小角散射与大分子晶体学之间的相互作用，核酸结构，病毒成熟动力学，基于原子结构的建模方法，膜系统，使用中子散射补充X射线研究，以及SSRL SMB计划的回顾和使用LCLS SMB研究的未来前景。Bio-SAXS/D工作人员讨论了BL 4 -2的发展，Bio-SAXS/D实验中的实际问题以及数据收集和处理的软件开发。研讨会的后半部分是两个半天的计算会议和实验辅导会议。计算实践会议由Dmitri Svergun（EMBL-Hamburg）提供，他的小组已经开发了许多强大的计算工具用于解决散射问题。选定的参与者有机会在BL 4 -2上单独分配的光束时间内测量自己的样品，许多人设法获得了令人鼓舞的结果。参加者对这些实用课程反应热烈，并与讲者和工作人员就生物小角X射线/衍射研究的实际问题进行了积极的讨论。一些讲习班参加者最近提交了快速获取或定期试验提案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL SMB bio-SAXS/D team hosted a practical workshop during July 28-30, 2006. The late July dates were chosen in order to include 6 beam time shifts of hands-on experimental tutorial sessions, complementing the last bio-SAXS/D workshop in October 2005. The first half of the workshop had oral presentations by invited expert speakers on mostly non-crystalline x-ray diffraction studies in structural biology, ranging from fundamentals of bio-SAXS/D techniques to cutting-edge advanced applications conducted at SSRL and elsewhere. These talks covered a comprehensive review of the structural biology research by SAXS/D techniques, interplay between small angle scattering and macromolecular crystallography, nucleic acid structures, virus maturation kinetics, a modeling approach based on atomic structures, membrane systems, the use of neutron scattering to complement x-ray studies, as well as a review of the SSRL SMB program and future prospect of SMB research using LCLS. Bio-SAXS/D staff discussed BL4-2 developments, practical issues in bio-SAXS/D experiments and software developments for data collection and processing. The second half of the workshop was devoted to two half-day computational sessions and experimental tutorial sessions. The computational practical sessions were given by Dmitri Svergun (EMBL-Hamburg), whose group has developed a number of powerful computational tools for solution scattering. Selected participants were given the opportunity to measure their own samples within a couple of hours of individually allocated beam time on BL4-2, and many managed to obtain encouraging results. These practical sessions were enthusiastically received by the participants, who engaged in active discussion with speakers and staff on practical issues in bio-SAXS/D studies. Some of the workshop participants have recently submitted Rapid Access or regular experimental proposals.