WORKSHOP ON SMALL-ANGLE X-RAY SCATTERING AND DIFFRACTION STUDIES IN STRUCTURAL B

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 SSRL SMB BIO-SAXS/D团队在2006年7月28-30日期间举办了一次实践研讨会。选择7月下旬的日期是为了包括动手实验教程课程的6个光束时移，以补充2005年10月举行的上一次BIO-SAXS/D研讨会。在讲习班的前半部分，特邀专家发言者作了口头陈述，主要是关于结构生物学中的非晶态X射线衍射研究，范围从生物-SAXS/D技术的基础到在SSRL和其他地方进行的尖端先进应用。这些讨论涉及SAXS/D技术的结构生物学研究，小角散射与大分子结晶学的相互作用，核酸结构，病毒成熟动力学，基于原子结构的建模方法，膜系统，利用中子散射补充X射线研究，以及SSRL SMB计划的回顾和利用LCLS进行SMB研究的未来展望。BIO-SAXS/D工作人员讨论了BL4-2的发展、BIO-SAXS/D实验中的实际问题以及数据收集和处理的软件开发。研讨会的后半部分专门用于两个半天的计算课程和实验辅导课程。计算实践课程由Dmitri Svergan(EMBL-Hamburg)提供，他的团队开发了许多用于溶液散射的强大计算工具。选定的参与者有机会在BL4-2上单独分配的光束时间的几个小时内测量他们自己的样本，许多人设法获得了令人鼓舞的结果。这些实践课程受到与会者的热烈欢迎，他们与演讲者和工作人员就BIO-SAXS/D研究中的实际问题进行了积极讨论。一些讲习班参与者最近提交了快速访问或定期实验提案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL SMB bio-SAXS/D team hosted a practical workshop during July 28-30, 2006. The late July dates were chosen in order to include 6 beam time shifts of hands-on experimental tutorial sessions, complementing the last bio-SAXS/D workshop in October 2005. The first half of the workshop had oral presentations by invited expert speakers on mostly non-crystalline x-ray diffraction studies in structural biology, ranging from fundamentals of bio-SAXS/D techniques to cutting-edge advanced applications conducted at SSRL and elsewhere. These talks covered a comprehensive review of the structural biology research by SAXS/D techniques, interplay between small angle scattering and macromolecular crystallography, nucleic acid structures, virus maturation kinetics, a modeling approach based on atomic structures, membrane systems, the use of neutron scattering to complement x-ray studies, as well as a review of the SSRL SMB program and future prospect of SMB research using LCLS. Bio-SAXS/D staff discussed BL4-2 developments, practical issues in bio-SAXS/D experiments and software developments for data collection and processing. The second half of the workshop was devoted to two half-day computational sessions and experimental tutorial sessions. The computational practical sessions were given by Dmitri Svergun (EMBL-Hamburg), whose group has developed a number of powerful computational tools for solution scattering. Selected participants were given the opportunity to measure their own samples within a couple of hours of individually allocated beam time on BL4-2, and many managed to obtain encouraging results. These practical sessions were enthusiastically received by the participants, who engaged in active discussion with speakers and staff on practical issues in bio-SAXS/D studies. Some of the workshop participants have recently submitted Rapid Access or regular experimental proposals.