STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 SSRL SMB小角度X射线散射站BL 4 -2致力于结构生物学研究，为蛋白质、蛋白质组装体、病毒颗粒、生物纤维和脂质膜的非晶体衍射和散射研究提供最先进的实验设施。2009年是BL 4 -2自大规模升级并搬迁至16区以来的第一个全年实验运行。一个反射镜间距反馈系统，以减轻光束不稳定性的问题，并导致在数据质量的显着改善。全面升级的In-hutch仪器具有可变距离针孔几何X射线散射相机系统，可配置为适应多种数据收集模式，包括溶液散射，单晶衍射，脂质/纤维衍射和倾斜入射散射。该系统覆盖了2 A至2000 A范围内的特征长度标度，具有七个离散的样品至检测器距离。一个Bonse-Hart几何超小角散射装置正在重新开发，以扩大范围高达几微米。最近获得的先进X射线探测器，Rayonix MX225 HE CCD和Dectris Pilatus 300 K硅像素阵列探测器，已充分表征并投入常规运行。前者用于BL 4 -2上的所有X射线散射/衍射实验，但后者涵盖的快速时间分辨研究除外。这两款探测器都表现出了卓越的性能和高可靠性。我们已经开发了几种新的样品处理设备，以提高数据吞吐量或促进新类型的实验，包括高吞吐量溶液X射线散射研究。我们还开发了一个温度控制的样品保持器的脂质夹心板（96孔微板格式），以及一个原型毛细管保持器的闪光光解实验。为了保护实验人员和探测人员不受伤害，研制了一种新的联锁人身保护系统

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL SMB small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for non-crystalline diffraction and scattering studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The year 2009 was the first full year of experimental run at BL4-2 since the extensive upgrades of the station and its relocation to Sector 16. A mirror pitch feedback system to mitigate beam instability issues was implemented, and resulted in a significant improvement in data quality. The fully upgraded in-hutch instrumentation features a variable-distance pin-hole geometry x-ray scattering camera system, which can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, lipid/fiber diffraction and gracing-incidence scattering. This system covers the characteristic length scale in the range of 2 A to 2000 A with seven discrete sample-to-detector distances. A Bonse-Hart geometry ultra-small angle scattering setup is being redeveloped to extend the range up to a few microns. Recently acquired advanced x-ray detectors, Rayonix MX225HE CCD and Dectris Pilatus 300K silicon pixel array detectors, were fully characterized and put in routine operation. The former is used all x-ray scattering/diffraction experiments on BL4-2 with the exception of fast time-resolved studies, which are covered by the latter. Both detectors have demonstrated exceptional performance and high reliability. We have developed several new sample handling devices to improve data throughtput or to facilitate new types of experiments, including for high thoughput solution x-ray scattering studies. We have also developed a temperature controlled sample holder for the lipid sandwich plate (96 well micro-plate format) as well as a prototype capillary holder for flash photolysis experiments. A new interlocked personnel protection system has been developed to protect experimenters and detectors from pote