STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
基本信息
- 批准号:7597911
- 负责人:
- 金额:$ 0.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-03-01 至 2008-02-29
- 项目状态:已结题
- 来源:
- 关键词:Active SitesAlcoholsAromatic HydrocarbonsCatalysisCatecholsComplexComputer Retrieval of Information on Scientific Projects DatabaseDioxygenEpoxy CompoundsFundingGrantHemeInstitutionMetalsMethane hydroxylaseMixed Function OxygenasesOxidoreductasePathway interactionsPhenol 2-monooxygenasePhenolsProteinsReactionResearchResearch PersonnelResourcesSourceStructureSystemUnited States National Institutes of Healthcarboxylategenetic regulatory proteinmutantoxidationreconstitutiontoluene 2-xylene monooxygenase
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Bacterial Multicomponent Monooxygenases (BMMs) such as methane monooxygenase (MMO), toluene/o-xylene monooxygenase (ToMO), and phenol hydroxylase (PH) catalyze the regio- and enantioselective oxidation of saturated and aromatic hydrocarbons to yield alcohols, phenols, catechols, and epoxides. Regulatory and reductase protein components are required to effect catalysis in BMM hydroxylases, which contain carboxylate-bridged non-heme diiron active sites where the oxidation reactions occur at a dioxygen-activated intermediate. Structures of the MMO hydroxylase in complex with the regulatory protein MMOB and the related inhibitory protein MMOD, together with MMOH-O2 intermediates, are sought to probe component interactions and reactive pathways in the MMO system. Structures of component protein complexes, oxygenated intermediates, and the diiron(II) reduced, metal-reconstituted, and mutant forms of ToMO and PH hydroxylases and their complexes will similarly be pursued.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
细菌多组分单加氧酶(Bacterial Multicomponent Monooxygenase,BMPs)如甲烷单加氧酶(Methane Monooxygenase,MMO)、甲苯/邻二甲苯单加氧酶(ToMO)和苯酚羟化酶(phenol hydroxylase,PH)催化饱和烃和芳烃的区域选择性和对映选择性氧化以产生醇、酚、儿茶酚和环氧化物。需要调节和还原酶蛋白组分来实现BMM羟化酶中的催化,BMM羟化酶含有羧酸根桥接的非血红素二铁活性位点,其中氧化反应发生在双氧活化的中间体处。MMO羟化酶与调节蛋白MMOB和相关抑制蛋白MMOD的复合物的结构,以及MMOH-O2中间体,试图探测MMO系统中的组分相互作用和反应途径。组分蛋白质复合物,含氧中间体,和二铁(II)还原,金属重组,和突变形式的ToMO和PH羟化酶及其复合物的结构将同样追求。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Stephen J. Lippard其他文献
X-ray structure of a dodecamer duplex containing the major cisplatin d(GpG) intrastrand cross-link
- DOI:
10.1016/0162-0134(95)97300-f - 发表时间:
1995-08-01 - 期刊:
- 影响因子:
- 作者:
Patricia M. Takahara;Amy C. Rosenzweig;Christin A. Frederick;Stephen J. Lippard - 通讯作者:
Stephen J. Lippard
Frank Albert Cotton (1930–2007)
弗兰克·艾伯特·科顿(1930 年至 2007 年)
- DOI:
10.1038/446626a - 发表时间:
2007-04-04 - 期刊:
- 影响因子:48.500
- 作者:
Stephen J. Lippard - 通讯作者:
Stephen J. Lippard
High resolution crystal structures of the hydroxylase protein of methane monooxygenase
- DOI:
10.1016/0162-0134(95)97479-a - 发表时间:
1995-08-01 - 期刊:
- 影响因子:
- 作者:
Amy C. Rosenzweig;Pär Nordlund;Stephen J. Lippard;Christin A. Frederick - 通讯作者:
Christin A. Frederick
Conjugués de nanoparticule de polynucléotide polyvalente en tant que véhicules de distribution pour un agent chimiothérapique
多核苷酸多价纳米粒子结合物与化学药物分配载体
- DOI:
- 发表时间:
2010 - 期刊:
- 影响因子:0
- 作者:
Chad A. Mirkin;David A. Giljohann;W. Daniel;Stephen J. Lippard;Shanta Dhar - 通讯作者:
Shanta Dhar
Structural, mechanistic, and model studies for methane monooxygenase
- DOI:
10.1016/0162-0134(95)97192-s - 发表时间:
1995-08-01 - 期刊:
- 影响因子:
- 作者:
Stephen J. Lippard - 通讯作者:
Stephen J. Lippard
Stephen J. Lippard的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Stephen J. Lippard', 18)}}的其他基金
STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
- 批准号:
8362193 - 财政年份:2011
- 资助金额:
$ 0.28万 - 项目类别:
INVESTIGATIONS OF CISPLATIN-DNA CROSS-LINKS ON NUCLEOSOME CORE PARTICLES
核小体核心颗粒上顺铂-DNA 交联的研究
- 批准号:
8169250 - 财政年份:2010
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
- 批准号:
8170154 - 财政年份:2010
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF MULTICOMPONENT BACTERIAL MONOOXYGENASES
多组分细菌单加氧酶的结构研究
- 批准号:
8169251 - 财政年份:2010
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
- 批准号:
7954158 - 财政年份:2009
- 资助金额:
$ 0.28万 - 项目类别:
CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGS
铂类抗癌药物的化学和生物学
- 批准号:
7955152 - 财政年份:2009
- 资助金额:
$ 0.28万 - 项目类别:
Nonheme Diiron Centers and the Biological Oxidation of Hydrocarbons
非血红素二铁中心和碳氢化合物的生物氧化
- 批准号:
7923548 - 财政年份:2009
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF MULTICOMPONENT BACTERIAL MONOOXYGENASES
多组分细菌单加氧酶的结构研究
- 批准号:
7955153 - 财政年份:2009
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
- 批准号:
7954496 - 财政年份:2009
- 资助金额:
$ 0.28万 - 项目类别:
STRUCTURAL STUDIES OF BACTERIAL MULTICOMPONENT MONOOXYGENASES
细菌多组分单加氧酶的结构研究
- 批准号:
7721732 - 财政年份:2008
- 资助金额:
$ 0.28万 - 项目类别:
相似海外基金
Collaborative Research: Overlooked Oxidation of Aqueous Alcohols: Kinetics, Mechanism, and Relevance to Water Reuse
合作研究:被忽视的水醇氧化:动力学、机制以及与水回用的相关性
- 批准号:
2304861 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Continuing Grant
STTR Phase I: Development of Modular Reactors to Convert Methane to Alcohols at Low Temperatures
STTR 第一阶段:开发在低温下将甲烷转化为醇的模块化反应器
- 批准号:
2151256 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Standard Grant
Development of amine-dehydrogenase and lyase biocatalysts for the sustainable manufacturing of unnatural chiral amino acids and amino alcohols
开发胺脱氢酶和裂解酶生物催化剂,用于可持续生产非天然手性氨基酸和氨基醇
- 批准号:
2870226 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Studentship
Collaborative Research: Overlooked Oxidation of Aqueous Alcohols: Kinetics, Mechanism, and Relevance to Water Reuse
合作研究:被忽视的水醇氧化:动力学、机制以及与水回用的相关性
- 批准号:
2304860 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Continuing Grant
Postdoctoral Fellowship: MPS-Ascend: Development of Selective Reaction Schemes for Photoactivation of Alcohols
博士后奖学金:MPS-Ascend:醇光活化选择性反应方案的开发
- 批准号:
2316541 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Fellowship Award
Development of phosphorylation of alcohols in protein based on the structural modification of phosphoenolpyruvate
基于磷酸烯醇丙酮酸结构修饰的蛋白质醇磷酸化研究进展
- 批准号:
22KJ1152 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Nickel Cross-Coupling Cascades with α-Heteroatom Radicals to Prepare Sterically Hindered Alcohols and Amines
镍与α-杂原子自由基交叉偶联级联制备位阻醇和胺
- 批准号:
10604535 - 财政年份:2023
- 资助金额:
$ 0.28万 - 项目类别:
Towards a better understanding of the effect of the pentafluorosulfanyl group on the lipophilicity and acid/base properties of alcohols and amines
更好地了解五氟硫基对醇和胺的亲脂性和酸/碱性质的影响
- 批准号:
571856-2021 - 财政年份:2022
- 资助金额:
$ 0.28万 - 项目类别:
Alliance Grants
Pd-Catalyzed C(sp3)-H Functionalizations Directed by Free Alcohols and Boc-Protected Amines
由游离醇和 Boc 保护的胺引导的 Pd 催化 C(sp3)-H 官能化
- 批准号:
10606508 - 财政年份:2022
- 资助金额:
$ 0.28万 - 项目类别:
MPS-Ascend: Nickel/Photoredox-Catalyzed C(sp3)–C(sp3) Cross-Coupling Between Alkyl Halides and Activated Alcohols
MPS-Ascend:镍/光氧化还原催化的 C(sp3)→C(sp3) 烷基卤化物和活化醇之间的交叉偶联
- 批准号:
2213210 - 财政年份:2022
- 资助金额:
$ 0.28万 - 项目类别:
Fellowship Award