INSULIN SECRETION FROM PANCREATIC ISLET BETA CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 还原型NAD(P)H和NADH在胰岛细胞胰岛素分泌调节中起重要作用。胞质和线粒体代谢以及由此导致的两个池中还原当量的变化是这一过程中的重要因素。 为了进一步阐明胞浆和线粒体成分的作用，我利用配备双光子激光的Zeiss LSM共聚焦显微镜研究了整个小鼠和大鼠胰岛对胰岛素分泌激素、葡萄糖和唯一的线粒体底物琥珀酸的NAD(P)H反应。 这项工作是作为Peter Smith博士在生物电流研究中心的实验室中心脏博士草奖学金的一部分完成的，在她作为研究助理科学家加入该小组之前。 在奖学金项目中，Emma能够收集数据，显示线粒体燃料琥珀酸对大鼠和小鼠NAD(P)H水平的不同影响，这两种动物的胞质代谢程度与线粒体代谢程度不同。这项工作将有助于理解胰岛素分泌不同胰岛素分泌的分子机制，以及如果这一功能在2型糖尿病条件下最终受损。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reducing equivalents, NAD(P)H and NADH play important roles in regulation of insulin secretion from pancreatic islet cells. Both cytosolic and mitochondrial metabolism and resulting changes in reducing equivalents in both pools are important factors in this process. To further elucidate the role of cytosolic and mitochondrial components, I have been studying NAD(P)H responses of whole mouse and rat pancreatic islets to insulin secretaqoques, glucose and the exclusive mitochondrial substrate succinate, using Zeiss LSM confocal microscope equipped with 2-photon laser which I have used for excitation of NAD(P)H. This work was done as a part of Dr Heart's Grass Fellowship in the lab of Dr. Peter Smith at the BioCurrents Research Center, prior to her joining the group as a Research Assistant Scientist. In the fellowship project Emma was able to collect data showing differential effect of mitochondrial fuel succinate on NAD(P)H levels in rat and mouse, which have a different degree of cytosolic versus mitochondrial metabolism. This work will help in understanding the molecular mechanism underlying insulin secretion to different insulin secretaqoques and ultimately impairment if this function in the type 2 diabetic conditions.