LUMINESCENT IMAGING: TARGETING ATP

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 发光(光子发射)记者与更传统的荧光记者相比，提供了不同的细胞活动视角，后者需要激发频率才能反应。尽管发光记者的多样性要小得多，但它们依赖于两个分子之间的化学相互作用，而这种相互作用取决于要测量的目标。在这里开发的案例中，我们希望通过发光荧光素/荧光素酶反应来记录ATP的细胞活性。荧光素是膜通透性的，由于荧光素酶是一种蛋白质，如果有合适的载体，它可以靶向和表达。获取这样的载体，并在我们的光子计数/成像系统中测试它们(见SPID 0094)是这个子项目的目标。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Luminescent (photon emitting) reporters offer different perspectives on cell activity than the more conventional fluorescent reporters  the latter requiring an excitation frequency to react. Luminescent reporters, although far less diverse, depend upon and chemical interaction between two molecules where the association is dependent on the target to be measured. In the case being developed here we wish to record the cellular activity of ATP through the photon emitting luciferin/luciferase reaction. Luciferin is membrane permeable and as luciferase is a protein it can targeted and expressed if a suitable vector is available. Acquisition of such vectors, and their testing in our photon counting/imaging systems (see SPID 0094) are the goals of this subproject.