EFFECT OF CHOLESTEROL REMOVAL ON PLA2 BINDING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 钙离子载体处理和去除胆固醇后红细胞膜和淋巴细胞膜的变化研究。用Laurdan法测定了膜的性能。胆固醇是由甲基-β-环糊精操纵的。用俄勒冈州绿色标记的sPLA2评估sPLA2与细胞的结合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Studies of changes in erythrocyte and lymphocyte cell membranes upon treatment with calcium ionophore and removal of cholesterol. Membrane properties are measured with laurdan. Cholesterol is manipulated with methyl-beta-cyclodextrin. The binding of sPLA2 to the cells is assessed with oregon green-labeled sPLA2.