EFFECT OF INTRACELLULAR CALCIUM ON THE ADSORPTION OF PLA2 TO MEMBRANE

细胞内钙对 PLA2 膜吸附的影响

• 批准号: 7600934
• 负责人: JOHN BELL
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7600934
• 关键词: Adsorption; Calcium; Cell membrane; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Hydrolysis; Institution; Ionomycin; Ionophores; Label; Lymphoma; Measures; Membrane; NBD-PC; Oregon; Pharmaceutical Preparations; Phospholipids; Predisposition; Research; Research Personnel; Resources; Source; Surface; United States National Institutes of Health; analog; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Measured adsorption of Oregon Green-labeled App D49 sPLA2 to the surface of S49 lymphoma cells. Measured labeling of cell membrane with the phospholipid analogue NBD-PC, then measured extraction of NBD-PC with sPLA2 and with BSA. Each of these experiments was performed with and without treating the cells with the drug ionomycin, a calcium ionophore that induces susceptibility to hydrolysis of membrane phospholipids by sPLA2.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 测定俄勒冈绿标记的App D49单磷脂酶A2在S49淋巴瘤细胞表面的吸附。用磷脂类似物NBD-PC标记细胞膜，再用sPLA2和BSA提取NBD-PC。这些实验中的每一个都是在用药物离子霉素处理细胞和不用药物离子霉素处理的情况下进行的，离子霉素是一种钙离子载体，可以诱导sPLA2对膜磷脂的水解敏感。