BIOPHYSICAL CHANGES IN CELL MEMBRANES DURING APOPTOSIS & CELLULAR TRAUMA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分泌型磷脂酶A2(SPLA2)与磷脂双层结合，催化磷脂的水解。正常情况下，细胞抵抗酶的作用，但在细胞凋亡或创伤期间变得容易受到影响。我们研究组的重点是了解真核细胞在受到细胞内钙离子升高的创伤或通过各种方式诱导凋亡时，细胞膜发生了什么物理变化。目的是了解膜结构与细胞死亡的关系，以及膜结构与磷脂酶A2作用的关系。用双光子显微镜研究了Laurdan和帕提曼等膜探针的荧光，以区分发生在质膜上的变化和与内膜有关的变化。此外，这些技术使我们能够确定膜特性的横向空间分布及其与细胞凋亡和酶活性的关系。在过去，这项工作一直局限于静态测量。已经有计划开始使用FCS来评估在细胞凋亡和创伤过程中细胞膜特性的潜在变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Secretory phospholipase A2 (sPLA2) binds to lipid bilayers and catalyzes hydrolysis of phospholipids. Normally, cells resist the enzyme's action, but they become susceptible during apoptosis or trauma. The focus of our research group is to understand what physical changes occur in the membranes of eukaryotic cells when they are traumatized by elevated intracellular calcium or induced into apoptosis by various means. The purpose is to understand the relationship between membrane structure and cell death and between membrane structure and the action of phospholipase A2. The fluorescence of membrane probes such as laurdan and patman are studied by two-photon microscopy to separate changes occuring at the plasma membrane from those that pertain to internal membranes. Moreover, these techniques allow us to identify the lateral spatial distribution of membrane properties and their relationship to apoptosis and enzyme activity. In the past, the work has been confined to static measurements. Plans are in place to begin using FCS to assess potential changes in the dynamics of membrane properties during apoptosis and trauma.